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Offlineinvitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
Re: Best spore printing tek [Re: MushroomWizard420] * 1
    #19955509 - 05/07/14 06:53 PM (9 years, 8 months ago)

Well there is not one shread of butthurt in my posts, and from where I stand he is giving seriously bad advice that will mess up prints for a lot of nubs.


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InvisiblePastywhyteMDiscord
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Re: Best spore printing tek [Re: 36fuckin5]
    #19955510 - 05/07/14 06:53 PM (9 years, 8 months ago)

Quote:

36fuckin5 said:
The difference is that your fruit was never sterile to begin with. The foil was. What we can't flame, we wipe with alcohol. You can't flame scissors because the handle will melt, or you'll burn yourself on the handle. Scalpel blades are super thin and cool quickly.




^^^This

We fruit in open air. Which means making syringes out of prints fruited in open air is a risky proposition. It can be done, does not mean it should be done. We also spray out hands down with iso before SAB work. Its not perfect but I personally don't feel like flame sterilizing my hands :shrug:

Unless your using a invitro tek like Violets, you should treat prints as if they are dirty. I have never picked up a contam on a plate I streaked with a print I took myself, but I would still always make at least one transfer before inoculation to ensure a clean culture.


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Offlineinvitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
Re: Best spore printing tek [Re: Pastywhyte]
    #19955519 - 05/07/14 06:55 PM (9 years, 8 months ago)

Quote:

Pastywhyte said:
Quote:

36fuckin5 said:
The difference is that your fruit was never sterile to begin with. The foil was. What we can't flame, we wipe with alcohol. You can't flame scissors because the handle will melt, or you'll burn yourself on the handle. Scalpel blades are super thin and cool quickly.




^^^This

We fruit in open air. Which means making syringes out of prints fruited in open air is a risky proposition. It can be done, does not mean it should be done. We also spray out hands down with iso before SAB work. Its not perfect but I personally don't feel like flame sterilizing my hands :shrug:

Unless your using a invitro tek like Violets, you should treat prints as if they are dirty. I have never picked up a contam on a plate I streaked with a print I took myself, but I would still always make at least one transfer before inoculation to ensure a clean culture.




So your saying, because it's not 100% clean it's ok to make the stem dirty whichs sit directly in the center of the print, risking the cleanliness of the print, risking the whole grow?  You want to talk about extra work?  How much extra work is it going to be to clean up those bacteria laden agar plates your about to make with that extra dirty print you just made??


Edited by invitro (05/07/14 06:57 PM)


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InvisiblePastywhyteMDiscord
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Re: Best spore printing tek [Re: invitro]
    #19955530 - 05/07/14 06:58 PM (9 years, 8 months ago)

I'm saying that if you use spore prints you take yourself, to use agar :shrug: Syringes are crap anyway. I did 12 cakes and said fuck it, I'm doing agar.

If you don't take your spores from the area where the stem was (shouldn't be any spores there anyway) and use a tiny amount, it will work out quite well 99% of the time.


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Offlineinvitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
Re: Best spore printing tek [Re: 36fuckin5] * 1
    #19955540 - 05/07/14 06:59 PM (9 years, 8 months ago)

Quote:

36fuckin5 said:
Quote:

36fuckin5 said:
Get off the forums and go read on microbiology from someone with a degree. scholar.google.com is your friend.








Done - found zero support for your position, anything else?


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OfflineMonkeyJesusFresco
am i suspended in agar?
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Registered: 10/09/12
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Re: Best spore printing tek [Re: invitro]
    #19955542 - 05/07/14 06:59 PM (9 years, 8 months ago)

I just bite the stipe off with my teeth, haven't had a single contam'd print yet :smirk:


--------------------
LAGM v 2.024
- endo cabendo


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Offlineinvitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
Re: Best spore printing tek [Re: Pastywhyte] * 1
    #19955560 - 05/07/14 07:03 PM (9 years, 8 months ago)

Quote:

Pastywhyte said:
I'm saying that if you use spore prints you take yourself, to use agar :shrug: Syringes are crap anyway. I did 12 cakes and said fuck it, I'm doing agar.

If you don't take your spores from the area where the stem was (shouldn't be any spores there anyway) and use a tiny amount, it will work out quite well 99% of the time.




I'm saying use agar with a print that has half the bacteria as the ones 365 makes, that way you waste less agar plates.  Syringes are only as crappy as the vendor you get them from.  taking spores from a certain area is your sure-fire solution?  You don't think that bacteria in the middle of the print can waft over to any other part of the print with the slightest air-current or bump from the cultivator?
How about when you fold the print, you don't that junk is getting everywhere?  How about not being lazy and flaming the scalpel or what have you?


Edited by invitro (05/07/14 07:03 PM)


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Invisible36fuckin5
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Registered: 08/11/03
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Re: Best spore printing tek [Re: invitro]
    #19955612 - 05/07/14 07:12 PM (9 years, 8 months ago)

How about you go fucking try it yourself before shooting shit down. This thread will be hidden now.

Like I said, make shit harder on yourself if you want. Just stop touting it as fact when you've obviously never tried it. And stop telling all these noobs things as fact when you really don't know what you're talking about.

My way does NOT add bacteria. You just don't understand sterility as well as you think you do. I've made easily thousands of prints. When I do any prints, I generally fill my SAB to the point where I only have a tiny little space to work in. Easily 100 at a time. They're all pretty much clean.

What you think you know from reading and what happens in real-world environments are 2 different things. Why do you think NOBODY EVER says to sterilize scissors.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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InvisiblePastywhyteMDiscord
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Re: Best spore printing tek [Re: invitro] * 1
    #19955638 - 05/07/14 07:16 PM (9 years, 8 months ago)

Bacteria is not airborne in most cases and if you allow your print to dry properly that will kill most bacteria. Like I said, I have not yet had a print I took contam once streaked to agar, but I still treat it carefully. Personally I don't relish the idea of flaming my scissors 25-50 times when making prints.

Besides it won't matter if you flame sterilize or not, when you cut the stipe you are pushing any contams on the stipe itself across the area you just cut :shrug:


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Offlineinvitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
Re: Best spore printing tek [Re: 36fuckin5] * 1
    #19955739 - 05/07/14 07:35 PM (9 years, 8 months ago)

Quote:

36fuckin5 said:
How about you go fucking try it yourself before shooting shit down. This thread will be hidden now.

Like I said, make shit harder on yourself if you want. Just stop touting it as fact when you've obviously never tried it. And stop telling all these noobs things as fact when you really don't know what you're talking about.

My way does NOT add bacteria. You just don't understand sterility as well as you think you do. I've made easily thousands of prints. When I do any prints, I generally fill my SAB to the point where I only have a tiny little space to work in. Easily 100 at a time. They're all pretty much clean.

What you think you know from reading and what happens in real-world environments are 2 different things. Why do you think NOBODY EVER says to sterilize scissors.




All you had to say was that experience has proven you right despite what it seems like.  Getting all angry and throwing curse words around, that's something you might meditate on or get some counseling for cuz it didn't convince anyone of anything on this side and it's probably not helping you much either.


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Offlineinvitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
Re: Best spore printing tek [Re: Pastywhyte]
    #19955781 - 05/07/14 07:45 PM (9 years, 8 months ago)

Quote:

Pastywhyte said:
Bacteria is not airborne in most cases and if you allow your print to dry properly that will kill most bacteria. Like I said, I have not yet had a print I took contam once streaked to agar, but I still treat it carefully. Personally I don't relish the idea of flaming my scissors 25-50 times when making prints.

Besides it won't matter if you flame sterilize or not, when you cut the stipe you are pushing any contams on the stipe itself across the area you just cut :shrug:




Your still arguing by the logic that if it's a little dirty it's ok to make it more dirty.  This is poor logic and you should abandon it completely.  Bacteria is everywhere, and in the air in serious numbers, open a petri plate to the air and watch the bacteria grow.
When that bacterial laden scissor blade hits the stem, and that all dries out, it's not too hard to see it dropping down right into the print, the center, the sides etc.

My point once again is, flaming the scalpel is easy, it definitely reduces contams of all kinds not just bacteria.  It's not ok to make a print dirtier because it's dirty already.  It's pretty cut and dry, if 365 gets away with it ok, but like muda said, why cut corners if you don't have to?  Maybe after a bunch of prints I will realize it won't make a difference.


Edited by invitro (05/07/14 07:57 PM)


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InvisiblePastywhyteMDiscord
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Re: Best spore printing tek [Re: Pastywhyte]
    #19955849 - 05/07/14 07:59 PM (9 years, 8 months ago)

Quote:

invitro said:
Your still arguing by the logic that if it's a little dirty it's ok to make it more dirty.




Not at all. I'm arguing that the contams that you should be worried about are already present on the stipe and will be there whether you flame sterilize or not. The fact is that because the fruit was in open air, there are contams all around the stipe, falling down, regardless of what you cut it with. Short of suspending the cap over the print (and I've seen people do this) which would not be foolproof anyway, you are not going to prevent the odd contam from the side of the stipe from getting onto the print. So your flame sterilization is not contributing to a clean print in any meaningful way.

Quote:

Pastywhyte said:
Besides it won't matter if you flame sterilize or not, when you cut the stipe you are pushing any contams on the stipe itself across the area you just cut :shrug:




I am not one to normally cut corners but try making a few hundred prints, then use them and you will understand what I am talking about. There is a reason that every decent cultivator when discussing spore inoculations stresses "small amounts" regardless of whether talking about spore syringes, inoculating pf jars, inoculating agar, etc. We know its a crapshoot. But this time its in our favor. Fact is that with my method, I have yet to see a contam show up on a plate inoculated with one of my prints. I have seen far more on prints received from sponsors.

You really want to have a challenge, try using wild prints. Then you will know what cleaning a dirty print is all about.


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Offlineinvitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
Re: Best spore printing tek [Re: Pastywhyte]
    #19955874 - 05/07/14 08:05 PM (9 years, 8 months ago)

Quote:

Pastywhyte said:
Quote:

invitro said:
Your still arguing by the logic that if it's a little dirty it's ok to make it more dirty.


Fact is that with my method, I have yet to see a contam show up on a plate inoculated with one of my prints. I have seen far more on prints received from sponsors.





That statement hit the nail right on the head, that's all 365 needed to say to me.  I can accept that until such time as I test it out myself.  Way to keep your cool Pasty and thanks for the advice.

I don't care about rank or title or bla bla, I just want to hear results, I'm a pretty easy going if I can get some real results.


Edited by invitro (05/07/14 08:10 PM)


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InvisiblePastywhyteMDiscord
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Re: Best spore printing tek [Re: invitro]
    #19955897 - 05/07/14 08:10 PM (9 years, 8 months ago)

No worries man :super: when you test it out be sure to post pics of the subsequent grow :awesomenod:


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OfflineMonkeyJesusFresco
am i suspended in agar?
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Re: Best spore printing tek [Re: invitro]
    #19955901 - 05/07/14 08:10 PM (9 years, 8 months ago)

Pasty, if you keep responding like that, you'll never get your "Boss Ass Bitch" tag :smirk:


--------------------
LAGM v 2.024
- endo cabendo


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InvisiblePastywhyteMDiscord
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Re: Best spore printing tek [Re: MonkeyJesusFresco]
    #19955920 - 05/07/14 08:15 PM (9 years, 8 months ago)

Meh, as long as I can still be . . .


:kingcrankey:


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OfflineMonkeyJesusFresco
am i suspended in agar?
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Re: Best spore printing tek [Re: Pastywhyte]
    #19955929 - 05/07/14 08:17 PM (9 years, 8 months ago)

nope, there's only 360 degrees in a one man circle-jerk...soooo 360 + 5... nope, no room for any more I'm afraid :shrug:

edit:
SHUT YOUR WHORE MOUTH AND LISTEN TO MY WORDS!


--------------------
LAGM v 2.024
- endo cabendo


Edited by MonkeyJesusFresco (05/07/14 08:18 PM)


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InvisiblePastywhyteMDiscord
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Re: Best spore printing tek [Re: MonkeyJesusFresco]
    #19955932 - 05/07/14 08:18 PM (9 years, 8 months ago)

:wtfsonic:


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OfflineMonkeyJesusFresco
am i suspended in agar?
Male


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Re: Best spore printing tek [Re: Pastywhyte]
    #19955944 - 05/07/14 08:21 PM (9 years, 8 months ago)

:foreverbatman:


--------------------
LAGM v 2.024
- endo cabendo


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Invisibleblindingleaf
blue collar underworld
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Re: Best spore printing tek [Re: MonkeyJesusFresco]
    #19955983 - 05/07/14 08:27 PM (9 years, 8 months ago)

:lol: wtf is going on in this thread :rofl:


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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