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OfflineCepheus
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Best spore printing tek
    #5922899 - 08/01/06 07:27 PM (17 years, 5 months ago)

My casing has some very tasty looking mushrooms that veils have just torn.

What is the easiest way to take a sterile enough spore print to be used in a syringe at a later date? I possess no special equipment (like gloveboxes etc)

I have taken prints before but have been unsuccessful at taking a sterile one :frown:


--------------------
"I only ever hope to reach equilibrium, in Nature's matrix, in line with the meridian" ~ Jehst

:sun: "...and I know that I have to keep breathing, as tomorrow the sun will rise, who knows what the tide will bring?" :sun:

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Send any spare spore prints you might have and help the distribution :grin:

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Invisibleliamtheloser
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Posts: 1,453
Re: Best spore printing tek [Re: Cepheus]
    #5923112 - 08/01/06 08:36 PM (17 years, 5 months ago)

small closed room, properly sterilized. wipe a sheet of foil with alcohol to sterlize it, place the caps on the foil, then cover each cap with a sterilized (with alcohol) shot glass (or drinking glass, w/e)

wait 24 hours, re-sterilize the small closed room, wear sterile gloves, pick up the shot glass, carefully remove cap, fold foil over the top of the print, fold the edges in so it's a sealed little square, then do that for every print you're taking.


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Offlinecivility
Stranger

Registered: 01/17/14
Posts: 43
Last seen: 7 years, 11 months
Re: Best spore printing tek *DELETED* [Re: liamtheloser]
    #19954235 - 05/07/14 02:01 PM (9 years, 8 months ago)

Post deleted by civility

Reason for deletion: N/A



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OfflineMushroomWizard420
mycological magician
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Registered: 04/03/14
Posts: 307
Loc: space Flag
Last seen: 1 year, 9 months
Re: Best spore printing tek [Re: civility]
    #19954434 - 05/07/14 02:42 PM (9 years, 8 months ago)

Quote:

civility said:
do i need to "dry", in a sense, the print before i fold down the edges? or will the print be relatively dry enough?


the alcohol will evaporate off of the foil real quick, definitely before the mushroom is done printing itself, so it should need dried anymore unless you do it underwater.
and don't do that^


--------------------
"And on the last day, God purchased RogerRabbit's DVD set, and learned to grow mushrooms. And it was good."
-Shroomesis 4:20

                                  :supershroom: :supershroom: :supershroom: :kingtard::supershroom: :supershroom: :supershroom:
                                  :poison: MushroomWizard420 :poison:
                                              :shroomin:
                               
          The spirit of the forest watches over his children as they grow...


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Invisible36fuckin5
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Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
Trusted Cultivator
Re: Best spore printing tek [Re: MushroomWizard420]
    #19954476 - 05/07/14 02:52 PM (9 years, 8 months ago)

That method is totally fucked.

First, use a SAB. You can't get away with making clean prints without one.

Second, you're not gonna be sterilizing a room without dropping thousands of dollars.

Third, wiping foil (which is already sterile) with alcohol is like washing your face then wiping shit on it. Alcohol doesn't sterilize anything, it only sanitizes. Big difference.

What you need to do is make a SAB. Take your roll of foil in there (after wiping the outside with alcohol) and rip off the first foot or so. Throw that away, it's touched open air and is dirty.

Take the next little bit of foil (however much you need depends on how many prints you want) and place it on the bottom of your SAB., shiny side up.

Take a paper clip and bend it so you have a little hook. Wipe this with alcohol and hook your cap from the top, for a little handle. Using alcohol-wiped scissors, cut the cap off as close to the gills as you can get without touching them.
Then use your hook to transfer the cap onto your foil. Repeat until you're done.

Seal up your SAB and leave it to drop for 24 hours. After that time, pull the caps out and wait another 12-24 hours so it dries a bit. After they dry, take a brand new razor or scalpel in there and cut each print apart. From there I like to just bag them up open-faced in little zipper-top bags. Not sandwich bags, those are too big, look in the craft section at Wal-Mart. Your other option is to just carefully fold it so that the print is inside your sterile foil package, then fold the edges over a time or 2 to seal it up. A little piece of tape won't hurt either.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OfflineMushroomWizard420
mycological magician
Male


Registered: 04/03/14
Posts: 307
Loc: space Flag
Last seen: 1 year, 9 months
Re: Best spore printing tek [Re: 36fuckin5]
    #19954577 - 05/07/14 03:26 PM (9 years, 8 months ago)

And in swoops 36fuckin5 with some excellent tips and a condescending tone, securing the win for Team Perfect! :awesome:

seriously though, good tips 365. i didn't know that bit about alcohol, and you summed up the important parts well. helpful as always.. :thumbup:


--------------------
"And on the last day, God purchased RogerRabbit's DVD set, and learned to grow mushrooms. And it was good."
-Shroomesis 4:20

                                  :supershroom: :supershroom: :supershroom: :kingtard::supershroom: :supershroom: :supershroom:
                                  :poison: MushroomWizard420 :poison:
                                              :shroomin:
                               
          The spirit of the forest watches over his children as they grow...


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Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
Trusted Cultivator
Re: Best spore printing tek [Re: MushroomWizard420]
    #19954587 - 05/07/14 03:28 PM (9 years, 8 months ago)

Quote:

MushroomWizard420 said:
And in swoops 36fuckin5 with some excellent tips and a condescending tone, securing the win for Team Perfect! :awesome:

seriously though, good tips 365. i didn't know that bit about alcohol, and you summed up the important parts well. helpful as always.. :thumbup:




Haha, I never claimed to not be an asshole. I just claim that if I open my mouth I know what I'm talking about.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OfflineMdahmer
Aloysius devadander abercrombie
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Registered: 04/05/14
Posts: 2,516
Last seen: 6 years, 6 months
Re: Best spore printing tek [Re: 36fuckin5]
    #19954599 - 05/07/14 03:32 PM (9 years, 8 months ago)

I like that idea of using a whole sheet and just cutting up later. 36 mafia wut wut. :cool:


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OfflinePineal Crust
Feeling psilly.
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Registered: 11/27/13
Posts: 205
Last seen: 1 year, 10 months
Re: Best spore printing tek [Re: Mdahmer]
    #19954755 - 05/07/14 04:21 PM (9 years, 8 months ago)

:takingnotes: I use a 6qt shoebox sized tub instead of a SAB, very good info!


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OfflineMushroomWizard420
mycological magician
Male


Registered: 04/03/14
Posts: 307
Loc: space Flag
Last seen: 1 year, 9 months
Re: Best spore printing tek [Re: 36fuckin5]
    #19954808 - 05/07/14 04:34 PM (9 years, 8 months ago)

Quote:

36fuckin5 said:
Quote:

MushroomWizard420 said:
And in swoops 36fuckin5 with some excellent tips and a condescending tone, securing the win for Team Perfect! :awesome:

seriously though, good tips 365. i didn't know that bit about alcohol, and you summed up the important parts well. helpful as always.. :thumbup:




Haha, I never claimed to not be an asshole. I just claim that if I open my mouth I know what I'm talking about.



I think that comment actually sums up your personality entirely. and i mean that as a compliment lol. you've helped me a lot with posts and shit, and i envy your ability to be as blunt as feel like being whenever you want. brutal honesty can hurt while it helps :rofldrunk:


--------------------
"And on the last day, God purchased RogerRabbit's DVD set, and learned to grow mushrooms. And it was good."
-Shroomesis 4:20

                                  :supershroom: :supershroom: :supershroom: :kingtard::supershroom: :supershroom: :supershroom:
                                  :poison: MushroomWizard420 :poison:
                                              :shroomin:
                               
          The spirit of the forest watches over his children as they grow...


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Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
Trusted Cultivator
Re: Best spore printing tek [Re: Pineal Crust]
    #19954812 - 05/07/14 04:35 PM (9 years, 8 months ago)

The 6 qt box doesn't help anything. When you go to bag or fold them up they're gonna get fucked because they're exposed to open air. You NEED a SAB to make clean prints. No ways around it. I wouldn't even suggest a flowhood for this.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OfflinePineal Crust
Feeling psilly.
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Registered: 11/27/13
Posts: 205
Last seen: 1 year, 10 months
Re: Best spore printing tek [Re: 36fuckin5]
    #19954838 - 05/07/14 04:42 PM (9 years, 8 months ago)

:thumbup: I understand its importance now, thanks


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OfflineHappy Littletree
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Registered: 01/02/14
Posts: 1,386
Loc: slightly elevated from ea...
Last seen: 1 year, 2 months
Re: Best spore printing tek [Re: Pineal Crust]
    #19954916 - 05/07/14 05:01 PM (9 years, 8 months ago)

I seen a tek that I've been trying out with a little variation. Ive been pre-cutting my foil pieces to a reasonable size. Then pre-folding them and unfolding them to get the creases where I want. I then place all these in a bigger piece of foil and bake in the oven at 350 for like an hour. I then take the whole package still hot straight to the SAB unfold and take them out and put them on the rack.


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Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
Trusted Cultivator
Re: Best spore printing tek [Re: Happy Littletree]
    #19954923 - 05/07/14 05:03 PM (9 years, 8 months ago)

That's a waste of time. Foil is already sterile.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


Extras: Filter Print Post Top
Offlineinvitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
Re: Best spore printing tek [Re: 36fuckin5]
    #19954991 - 05/07/14 05:18 PM (9 years, 8 months ago)

Quote:

36fuckin5 said:
Using alcohol-wiped scissors, cut the cap off as close to the gills as you can get without touching them.




Aren't you risking transferring bacteria from the scissors to the stem here?  Flame sterilize a scalpel ?? They are cheap enough at a grow store $2.


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Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
Trusted Cultivator
Re: Best spore printing tek [Re: invitro]
    #19955056 - 05/07/14 05:32 PM (9 years, 8 months ago)

Scalpels suck for cutting caps. And alcohol-wiped scissors are much cleaner than the open air you fruit in.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


Extras: Filter Print Post Top
InvisibleBeside the Garden
Male User Gallery

Registered: 06/03/13
Posts: 606
Re: Best spore printing tek [Re: invitro]
    #19955058 - 05/07/14 05:32 PM (9 years, 8 months ago)

Ok I read on here months ago...

Use your sterilization box with the arm holes cut in it.
Have a few zip lock baggies in there, make sure every thing is a steril as posable.
Put a fresh cap into the baggie and let it sit.
After some time has passed and you get a good print remove the cap and seal it up.
When you need the print inject your solution into the baggie, shake then suck it backup into the syringe.
inoculate.

Anybody see any flaws in this or why it couldn't work. Never done it myself but it sounded pretty simple and clean.


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Offlineinvitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
Re: Best spore printing tek [Re: 36fuckin5]
    #19955070 - 05/07/14 05:34 PM (9 years, 8 months ago)

Quote:

36fuckin5 said:
Scalpels suck for cutting caps. And alcohol-wiped scissors are much cleaner than the open air you fruit in.




flame sterilize the scissors then?  1 bacteria in a print is one too many.


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Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
Trusted Cultivator
Re: Best spore printing tek [Re: invitro]
    #19955083 - 05/07/14 05:36 PM (9 years, 8 months ago)

Quote:

Beside the Garden said:
Ok I read on here months ago...

Use your sterilization box with the arm holes cut in it.
Have a few zip lock baggies in there, make sure every thing is a steril as posable.
Put a fresh cap into the baggie and let it sit.
After some time has passed and you get a good print remove the cap and seal it up.
When you need the print inject your solution into the baggie, shake then suck it backup into the syringe.
inoculate.

Anybody see any flaws in this or why it couldn't work. Never done it myself but it sounded pretty simple and clean.




It doesn't allow you to use agar. Fuck making spore syringes.

Quote:

invitro said:
flame sterilize the scissors then?  1 bacteria in a print is one too many.




You're not gonna ever get a 100% clean print no matter what you do. Maybe if you fruit invitro and keep it in front of a flowhood the entire time.

Again, the scissors are cleaner than your fruiting conditions.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


Extras: Filter Print Post Top
Offlineinvitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
Re: Best spore printing tek [Re: 36fuckin5]
    #19955098 - 05/07/14 05:38 PM (9 years, 8 months ago)

Op didn't ask for perfect tek he asked for best tek.  Best tek is flame sterilizing the scissors.


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