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Shop: Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag

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OfflineToxxy
Random Gary
Registered: 07/07/06
Posts: 36
Last seen: 16 years, 10 months
Innoculating with stalled LC?
    #5921639 - 08/01/06 11:52 AM (17 years, 5 months ago)

I've been working on a couple LCs, the first one made decent progress but seemed to stop after growing to about half the size of a ping pong ball, and the second was doing well, but now it's all bunched up and is going nowhere. I didn't put a stirring device in either, so maybe that's to blame.

Anyway, maybe it's my imagination, but it looks like the first one has grown a little bit since I brought it out of the incubator for isolation, so I'm thinking of going ahead and using it. If I were to inoculate a BRF jar with LC from there, what would be likely to happen?

Also, does anyone have any tips on sucking up LC when it's at the bottom of a jar that's filled up with water? I can't reach it with my syringe, and I think it's too full to reach by tipping.


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OfflineSinthetic
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Registered: 12/05/05
Posts: 812
Last seen: 15 years, 6 months
Re: Innoculating with stalled LC? [Re: Toxxy]
    #5921649 - 08/01/06 11:56 AM (17 years, 5 months ago)

What do you have the LC in? If the container doesn't have to much extra fluid, shake the hell out of it until the mycelium breaks up and just suck up some slurry.


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OfflineToxxy
Random Gary
Registered: 07/07/06
Posts: 36
Last seen: 16 years, 10 months
Re: Innoculating with stalled LC? [Re: Sinthetic]
    #5921688 - 08/01/06 12:09 PM (17 years, 5 months ago)

I have the LC in a halfpint jar, pretty much full to the top with karo-water. Shaking the hell out of it could be disastrous, since I've got micropore tape on the top of it. Should I just cover the hole with my finger and go nuts, or isn't that an invitation for contamination?


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OfflineSinthetic
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Registered: 12/05/05
Posts: 812
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Re: Innoculating with stalled LC? [Re: Toxxy]
    #5921706 - 08/01/06 12:16 PM (17 years, 5 months ago)

I would just change the tape when you're done.


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Invisiblecreamcorn
mad scientist
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Registered: 03/13/06
Posts: 2,962
Trusted Cultivator
Re: Innoculating with stalled LC? [Re: Sinthetic]
    #5921733 - 08/01/06 12:26 PM (17 years, 5 months ago)

suck up some liquid into your syringe, and forcefully squirt it back down.  will break up the cloud into bits no problem.  suck up a second time and you should capture plenty.

next time fill your jar about half way.  makes it easier to agitate, less likely to contam, and easier to reach the good stuff (by tilting)... if half a half pint doesnt seem like enough LC to you, make two, or use a bigger jar. :smile:


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