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Invisiblejellyfish

Registered: 10/02/05
Posts: 7,457
Long germination time?
    #5921467 - 08/01/06 10:55 AM (17 years, 5 months ago)

One week ago my cat brushed some spores into a petri dish of agar agar and malt. Everything was made sure as sterile and no contamination appeared. It's been a week and there is absoultly nothing on the agar still. They haven't been incubated but it's been pretty hot here so I didn't think that would be a problem. Is there any hope something will grow?


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OfflineSinthetic
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Registered: 12/05/05
Posts: 812
Last seen: 15 years, 6 months
Re: Long germination time? [Re: jellyfish]
    #5921490 - 08/01/06 11:01 AM (17 years, 5 months ago)

Germination can take 2 weeks or longer, especially if the spores are old.


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Invisiblefastfred
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Folding@home Statistics
Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
Re: Long germination time? [Re: Sinthetic]
    #5921587 - 08/01/06 11:35 AM (17 years, 5 months ago)

Yeah, it may take awhile. Your agar could be too stiff, 12.5g/L works good if you have difficulty germinating. If you embed the spores in the agar with a spreader it might also help.


-FF


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