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OfflineVelocity92C
Stranger
Registered: 07/22/06
Posts: 170
Last seen: 14 years, 9 months
Flame sterilization or alcohol?
    #5920204 - 07/31/06 10:16 PM (17 years, 5 months ago)

Which do you prefer? About to start my second batch - first time I tried flame but didn't have an alcohol lamp, which I do now. Is it easier just to wipe the tip of the needle with alcohol?


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Invisiblemonstermitch
Growing in Bags Doesn't Work

Folding@home Statistics
Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay Flag
Re: Flame sterilization or alcohol? [Re: Velocity92C]
    #5920214 - 07/31/06 10:18 PM (17 years, 5 months ago)

alcohol will not sterilize inside of the needle.

it you are sterile from the get go, you won't need to flame.

if you suspect anything inside the needle, flame.


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Offlineimtank
Self-Taught Fool
Male

Registered: 07/05/06
Posts: 235
Loc: somewhere around Barstow,...
Last seen: 16 years, 2 months
Re: Flame sterilization or alcohol? [Re: monstermitch]
    #5920294 - 07/31/06 10:42 PM (17 years, 5 months ago)

i never flamed and out of 30 jars so far i've only had 1 contam...you just have be careful...i wipe the tip after every jar and i spray oust after every couple...i also wear a dust mask


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'To be positive at all times is to ignore all that is important, sacred or valuable. To be negative at all times is to be threatened by ridiculousness and instant discredibility.'
-Kurt Kobain


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InvisibleAfroshroomerican
Oprah's Minion
Male

Registered: 05/12/06
Posts: 891
Loc: Pennsylvania
Re: Flame sterilization or alcohol? [Re: imtank]
    #5920305 - 07/31/06 10:45 PM (17 years, 5 months ago)

Umm as far as I know:

Flame - heats the inside of the needle so you get rid of the contams there.

Alchohol - airborne crap that couldve landed on the needle.

I thought it was best to do both and WAIT till the needle has cooled down and then wipe it with an alchohol swab to ensure it isn't contamed.

but i'd wait till the growing gurus answer


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"We must learn to live together as brothers or perish together as fools."

~Martin Luther King Jr.~

<passitbobbie> if I just showed you a closeup of my ass
<passitbobbie> youd think it was female

"You owe errrbody up in here an apology fow youwe shit, HO!" - classic


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Invisiblemonstermitch
Growing in Bags Doesn't Work

Folding@home Statistics
Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay Flag
Re: Flame sterilization or alcohol? [Re: Afroshroomerican]
    #5920318 - 07/31/06 10:52 PM (17 years, 5 months ago)



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Invisiblecreamcorn
mad scientist
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Registered: 03/13/06
Posts: 2,962
Trusted Cultivator
Re: Flame sterilization or alcohol? [Re: monstermitch]
    #5920322 - 07/31/06 10:54 PM (17 years, 5 months ago)

or buy a case of sealed presterilized needles so they come to a couple pennies a piece, use once and throw away, skip all that other noise :thumbup:

<--- lazy as heeell


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InvisibleAfroshroomerican
Oprah's Minion
Male

Registered: 05/12/06
Posts: 891
Loc: Pennsylvania
Re: Flame sterilization or alcohol? [Re: creamcorn]
    #5920335 - 07/31/06 10:58 PM (17 years, 5 months ago)

Quote:

RogerRabbit said:
The problem is the alcohol doesn't penetrate to the inside of the needle. I suggest flaming the needle red hot, then using the first couple of drops from the syringe to cool it down. That way, you know the inside and outside of the needle are sterile. In other words, heat it red hot, then allow to cool for only a few seconds and use.
RR




--------------------
"We must learn to live together as brothers or perish together as fools."

~Martin Luther King Jr.~

<passitbobbie> if I just showed you a closeup of my ass
<passitbobbie> youd think it was female

"You owe errrbody up in here an apology fow youwe shit, HO!" - classic


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OfflineYamidude
Stranger

Registered: 06/15/06
Posts: 957
Last seen: 15 years, 5 months
Re: Flame sterilization or alcohol? [Re: creamcorn]
    #5920344 - 07/31/06 11:00 PM (17 years, 5 months ago)

Quote:

creamcorn said:
or buy a case of sealed presterilized needles so they come to a couple pennies a piece, use once and throw away, skip all that other noise :thumbup:

<--- lazy as heeell




I loaded about 12 3cc pre-sterilized brand new syringes with LC last night.  I happenend to have a huge plastic tub of them though from my other projects..  Syringes are cheap as hell on the web..


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Invisiblecreamcorn
mad scientist
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Registered: 03/13/06
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Re: Flame sterilization or alcohol? [Re: Yamidude]
    #5920365 - 07/31/06 11:06 PM (17 years, 5 months ago)

wow 3cc talk about a tedious pain in the ass.  i use a 60cc for LC and 20cc for spores. :smile:

but yeah i dont wanna do no linking to non-sponsor sites but if you look around you can find syringes and needles dirt cheap... places that ship anywhere, quickly, no questions asked... unlike all the paranoia people have about going and buying them from somewhere in person...  i got 100 needles for like 7 bucks.  its not even worth trying to clean them or re-use them.  i still keep it wrapped up in an alcohol soaked paper towel between innoculations so its not exposed to the air, but definitely dont bother flaming... they're sterile out of the package, then i apply the garbage can tek!  even more peace of mind from a contam point of view, even less work from a lazy man's point of view.


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OfflineYamidude
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Registered: 06/15/06
Posts: 957
Last seen: 15 years, 5 months
Re: Flame sterilization or alcohol? [Re: creamcorn]
    #5920371 - 07/31/06 11:08 PM (17 years, 5 months ago)

i made the 3cc ones for some pint jars i have. I dont like using too much LC on the pints because i make my on the moist side already..


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Invisiblecreamcorn
mad scientist
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Re: Flame sterilization or alcohol? [Re: Yamidude]
    #5920391 - 07/31/06 11:16 PM (17 years, 5 months ago)

i put 2-3 in a half pint brf cake... 5-7 in a quart of wbs.  love sticking it in the middle of a wbs jar, spinning the jar around as i squirt away, checking back 48 hours later and seeing almost the whole surface against the glass already done all the way around. :smile:

i just hate making spore syringes, so when i do it, i do it up.  i also dont like to have to open my LCs for long nor stick things into them repeatedly, so when i dip in for some i dip in for a lot.  i hated working with 10cc syringes... id go insane with 3cc ones :smile:


and why on earth would you make your jars on the moist side.  it only slows them down and encourages bacteria.  there's plenty of time to add moisture after colonization!


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OfflineYamidude
Stranger

Registered: 06/15/06
Posts: 957
Last seen: 15 years, 5 months
Re: Flame sterilization or alcohol? [Re: creamcorn]
    #5920403 - 07/31/06 11:18 PM (17 years, 5 months ago)

Quote:

creamcorn said:
i put 2-3 in a half pint brf cake... 5-7 in a quart of wbs.  love sticking it in the middle of a wbs jar, spinning the jar around as i squirt away, checking back 48 hours later and seeing almost the whole surface against the glass already done all the way around. :smile:

i just hate making spore syringes, so when i do it, i do it up.  i also dont like to have to open my LCs for long nor stick things into them repeatedly, so when i dip in for some i dip in for a lot.  i hated working with 10cc syringes... id go insane with 3cc ones :smile:


and why on earth would you make your jars on the moist side.  it only slows them down and encourages bacteria.  there's plenty of time to add moisture after colonization!




iono but i have 0 contams a no prob with colonization times??


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Invisiblecreamcorn
mad scientist
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Registered: 03/13/06
Posts: 2,962
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Re: Flame sterilization or alcohol? [Re: Yamidude]
    #5920420 - 07/31/06 11:20 PM (17 years, 5 months ago)

well if it works keep on doing it.

maybe they're not as moist as you think.


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OfflineYamidude
Stranger

Registered: 06/15/06
Posts: 957
Last seen: 15 years, 5 months
Re: Flame sterilization or alcohol? [Re: creamcorn]
    #5920434 - 07/31/06 11:22 PM (17 years, 5 months ago)

Quote:

creamcorn said:
well if it works keep on doing it.

maybe they're not as moist as you think.


probably not..  Iono im still getting used to the way WBS looks after pc'ing.  But i guess im doing it right because i have fully colonized and spawned 15 jars already i have 3 LC's going and i just innocced 12 more jars last night as well as two bigass myco bags i made up with WBS, some BRF and some coffee just for fun.. :thumbup:


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OfflineFrappy
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Registered: 09/17/04
Posts: 1,280
Loc: South Florida
Last seen: 17 years, 3 months
Re: Flame sterilization or alcohol? [Re: Yamidude]
    #5921023 - 08/01/06 04:34 AM (17 years, 5 months ago)

Flame sterilization AND alcohol!!  :fireworship: :fireworship: :fireworship:

Burnin' down the house!!


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Invisiblebaltazar
Quiet dreamer

Registered: 02/23/04
Posts: 746
Re: Flame sterilization or alcohol? [Re: Frappy]
    #5921150 - 08/01/06 06:12 AM (17 years, 5 months ago)

I allways flame the needle first, than wipe it with alcohol (that cools it off too), wait a little and then inoculate... works great


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Offlinetonyperez420
Shaman Rasta

Registered: 11/03/04
Posts: 1,234
Last seen: 10 years, 11 months
Re: Flame sterilization or alcohol? [Re: Frappy]
    #5921214 - 08/01/06 07:40 AM (17 years, 5 months ago)

Quote:

Frappy said:
Flame sterilization AND alcohol!!  :fireworship: :fireworship: :fireworship:

Burnin' down the house!!




I do BOTH

First 70% isopryl rubbing alcohol, generously

THEN

With bics lighter, zippo (doesnt matter) I flame 3/4 of the needle, especially the tip until it turns red, then inoculate as soon as possible

month of July did almost 200 innoculations with only 2 contams

thank god,allah, buddah for FHs :crazy2:


Edited by tonyperez420 (08/01/06 07:41 AM)


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OfflineFrappy
Strange

Registered: 09/17/04
Posts: 1,280
Loc: South Florida
Last seen: 17 years, 3 months
Re: Flame sterilization or alcohol? [Re: tonyperez420]
    #5921696 - 08/01/06 12:12 PM (17 years, 5 months ago)

Thats a pretty good ratio. Just make sure your needle isn't so hot that the spore solution isn't boiling then it passes through the lumen of the needle. Otherwise the spores wouldn't be too viable.

Also, noobs, please be careful when working with alcohol and flame. Use common sense.


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