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nishikkiknee
journeyman
Registered: 05/21/01
Posts: 114
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Old spore prints, germinating
#5915335 - 07/30/06 03:38 PM (17 years, 6 months ago) |
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Hey folks,
Long time no see !
I had to take a mandatory vacation for about 5 years, anyhow my gorilla freind had some old prints from 2001 and he made a spore syringe and tried to germinate some on agar but they just wont take off, i read a section from stamets Mushroom Cultivator to him, where it said that old prints can be germinated by adding them to sterile water and holding them under 15 psi of pressure anybody ever do this or know how to do this ? my gorilla buddy is itching to take a trip, but he can't get his spores to germinate and he can't afford to purchase a new print at this time. any help on getting these things to go would greatly be appreciated.
Edited by nishikkiknee (08/21/06 11:05 AM)
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monstermitch
Growing in Bags Doesn't Work


Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay
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Re: Old spore prints, germinating [Re: nishikkiknee]
#5915346 - 07/30/06 03:43 PM (17 years, 6 months ago) |
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when germinating spores on agar...
I use MEA agar to start. and mix in one teaspoon of powdered activated carbon into each liter of agar.
this should give you a hand.
edit: also try liquid culture.
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nishikkiknee
journeyman
Registered: 05/21/01
Posts: 114
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Re: Old spore prints, germinating [Re: monstermitch]
#5916461 - 07/30/06 09:00 PM (17 years, 6 months ago) |
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ok, my gorilla friend tried starting on agar, spores were rehydrated in water for 48 hrs. Now tell what it is that the activated charcoal does. My gorilla frien and i have never heard of this does it help to rehydrate spore and to the mineral composition of the agar what, now were puzzled.
Later levi
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fastfred
Old Hand



Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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Re: Old spore prints, germinating [Re: nishikkiknee]
#5918024 - 07/31/06 09:04 AM (17 years, 5 months ago) |
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5 years is nothing. I've had 10 year old spores germinate. Someone else reported 20+ year old spores germinating.
1.5% malt extract, 0.5% dextrose in an LC worked for me. Try putting a piece of the print into the LC if you have some antibiotics in the LC or the print is still clean.
-FF
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nishikkiknee
journeyman
Registered: 05/21/01
Posts: 114
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Re: Old spore prints, germinating [Re: fastfred]
#5918692 - 07/31/06 02:21 PM (17 years, 5 months ago) |
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thank you, My gorilla buddy, did up anew batch of agar lastnight and he says he'll try the liquid culture. he thinks maybe they should have been left to hydrate a little longer than 48 hrs, so he says he's gonna put some in sterile water for about a week and have another go.
once again thank you kindly
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
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Re: Old spore prints, germinating [Re: nishikkiknee]
#5918710 - 07/31/06 02:28 PM (17 years, 5 months ago) |
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Hydrating old spores under pressure in a malt extract slurry. 5 PSI above ambient is plenty to force moisture through the cell wall. If you decide to go higher, I would suggest something other than glass as you see here. My nerves/common sense wouldn't allow me to pump it past 7 PSI, and even then I had it wrapped up inside a heavy rubber mat. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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em_bre_O
shroomery'sEmbryoticAsshole


Registered: 05/27/02
Posts: 2,312
Loc: In the stages of develope...
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Re: Old spore prints, germinating [Re: RogerRabbit]
#5918724 - 07/31/06 02:36 PM (17 years, 5 months ago) |
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Hey Rodger.....Didn't paul stamets get some of the spores from the ice man mummy to germ.? I'm pretty sure that's what he said when I was there.
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nishikkiknee
journeyman
Registered: 05/21/01
Posts: 114
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Re: Old spore prints, germinating [Re: RogerRabbit]
#5918867 - 07/31/06 03:17 PM (17 years, 5 months ago) |
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Quote:
RogerRabbit said: Hydrating old spores under pressure in a malt extract slurry. 5 PSI above ambient is plenty to force moisture through the cell wall. If you decide to go higher, I would suggest something other than glass as you see here. My nerves/common sense wouldn't allow me to pump it past 7 PSI, and even then I had it wrapped up inside a heavy rubber mat. RR
thats exactly what i was lookong for, my gorilla buddy will be so happy.
Thanks kindly
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Cloneufc
Master Exploder!



Registered: 11/15/07
Posts: 1,237
Loc: Las Vegas
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Re: Old spore prints, germinating [Re: nishikkiknee]
#11042225 - 09/11/09 06:37 PM (14 years, 4 months ago) |
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I know this is an old post but Im having a problem germinating a spore print that is only 2 years old. I thought I was getting pretty close to knowing most everything about mycology until I saw this lol. Where can I get one of these pressure containers? DO any of the sponsors sell them? Do I need to make my own? How can spores germinate under pressure without gas exchange?
Edited by Cloneufc (09/11/09 06:40 PM)
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veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
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Re: Old spore prints, germinating [Re: Cloneufc]
#11042257 - 09/11/09 06:43 PM (14 years, 4 months ago) |
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Could probbably be made. Just looks like a jar that has a pressure gauge and a 1 way valve fitted. Possibly a valve that you can attach a bicycle pump too.
Just a guess though.
I'm not sure how much pressure a regulart jar will stanf up too
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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Cloneufc
Master Exploder!



Registered: 11/15/07
Posts: 1,237
Loc: Las Vegas
Last seen: 5 months, 29 days
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Re: Old spore prints, germinating [Re: veda_sticks]
#11042274 - 09/11/09 06:47 PM (14 years, 4 months ago) |
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I think a schrader valve might work. Thanks for the idea. I can then use a bicycle pump and a $2 dollar pressure gauge. This technique is fascinating because its the next step before snake venom and something anyone can do or make.
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veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
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Re: Old spore prints, germinating [Re: Cloneufc]
#11042286 - 09/11/09 06:49 PM (14 years, 4 months ago) |
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Its very interesting and the first I have ever heard of it. Makes sense really. You kind of do the same thing to mycelium when dunking substrate by having it submerged underwater.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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Cloneufc
Master Exploder!



Registered: 11/15/07
Posts: 1,237
Loc: Las Vegas
Last seen: 5 months, 29 days
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Re: Old spore prints, germinating [Re: veda_sticks]
#11042298 - 09/11/09 06:52 PM (14 years, 4 months ago) |
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How can spores germinate under pressure without gas exchange? Maybe RR can explain that?
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veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 25 days
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Re: Old spore prints, germinating [Re: Cloneufc]
#11042314 - 09/11/09 06:56 PM (14 years, 4 months ago) |
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Think about it!
Why do you need gas exchange? Because growing mycelium breathes oxygen and gives off c02. At this point there is neglibable gas production. Gas exchange doesn't become much of a problem untill there's enog mycelium to raise co2 levels.
Use your noggin.
I'm sure most people in the beginning noticed that with jars, leaving the foil own and not noticing slow/stalled growth till later on
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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Cloneufc
Master Exploder!



Registered: 11/15/07
Posts: 1,237
Loc: Las Vegas
Last seen: 5 months, 29 days
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Re: Old spore prints, germinating [Re: veda_sticks]
#11042347 - 09/11/09 07:05 PM (14 years, 4 months ago) |
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Yeah thats sounds right, I got real excited and couldnt think. I'll try this out with a pressurized LC.
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myceleus_rex
seeker



Registered: 07/01/09
Posts: 1,581
Loc: alized
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Re: Old spore prints, germinating [Re: Cloneufc]
#11042726 - 09/11/09 08:26 PM (14 years, 4 months ago) |
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Hmm,
Maybe use something made out of PTFE, like a plastic soda bottle, they can handle 100 psi easy and with no shrapnel hazard. Use a wide-mouth pop bottle with a tire valve in the cap and you can drop a syringe prepped in sterile conditions right inside. Take it right up to 20 psi in safety and voila...
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Cloneufc
Master Exploder!



Registered: 11/15/07
Posts: 1,237
Loc: Las Vegas
Last seen: 5 months, 29 days
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Re: Old spore prints, germinating [Re: myceleus_rex]
#11043793 - 09/11/09 11:48 PM (14 years, 4 months ago) |
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A schrader valve is a tire valve. I went to the store and found a plastic cookie jar with a screw lid. 
I can fit my sterile LC inside the cookie jar, since it has micropore tape covering the holes.
Edited by Cloneufc (09/11/09 11:51 PM)
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myceleus_rex
seeker



Registered: 07/01/09
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Re: Old spore prints, germinating [Re: Cloneufc]
#11043908 - 09/12/09 12:13 AM (14 years, 4 months ago) |
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There ya go...
I only wonder if the cookie jar will pressurize well without leaks. Pop bottles are designed for pressure. Post back with results.
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