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OfflineLeanin
Student of theIron Game
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Registered: 04/18/06
Posts: 2,231
Last seen: 10 years, 9 months
without sterilizing/pasteurizing
    #5907306 - 07/27/06 11:04 PM (17 years, 8 months ago)

hey, has anyone had any success with not sterilizing/pasteurizing casing layer, substrate, or spawn?

what do you use?

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OfflineYamidude
Stranger

Registered: 06/15/06
Posts: 957
Last seen: 15 years, 7 months
Re: without sterilizing/pasteurizing [Re: Leanin]
    #5907314 - 07/27/06 11:06 PM (17 years, 8 months ago)

sure. You could get lucky but why bother wasting spore syringes or LC?

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Invisiblemonstermitch
Growing in Bags Doesn't Work

Folding@home Statistics
Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay Flag
Re: without sterilizing/pasteurizing [Re: Yamidude]
    #5907364 - 07/27/06 11:19 PM (17 years, 8 months ago)

okay, try this:

put some mold, bacteria, and spores in a substrate.
no pasteurization or sterilization.

let them go.

who do you think will win?

closest you'll come is bluelou's black kow right out of the bag idea.
http://www.shroomery.org/forums/showflat.php/Number/4304709#Post4304709

but that is outside.
I think he uses sterilized spawn anyway,
not into reading up on that thread right now.
inside you don't stand a chance, at least not where I live.


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InvisibleTippinthru
contented

Registered: 04/07/05
Posts: 1,131
Loc: "The Garden"...
Re: without sterilizing/pasteurizing [Re: Leanin]
    #5907386 - 07/27/06 11:25 PM (17 years, 8 months ago)

Yaaaaa :thumbup:

Blue green mold, with wet spot following along :rolleyes:


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Perfection is attained by slow degrees; it requires the hand of time...
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OfflineSinthetic
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Registered: 12/05/05
Posts: 812
Last seen: 15 years, 8 months
Re: without sterilizing/pasteurizing [Re: Tippinthru]
    #5908203 - 07/28/06 07:29 AM (17 years, 8 months ago)

Sounds like someone doesn't want to get a PC.

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Offlinetonyperez420
Shaman Rasta

Registered: 11/03/04
Posts: 1,234
Last seen: 11 years, 1 month
Re: without sterilizing/pasteurizing [Re: Leanin]
    #5908237 - 07/28/06 07:44 AM (17 years, 8 months ago)

Quote:

Leanin said:
hey, has anyone had any success with not sterilizing/pasteurizing casing layer, substrate, or spawn?

what do you use?




maybe you can get away with it with your casing layer.... RogerRabbit boasts that he does not believe in sterilizing casings, but I always nuke my casings, so I am ignorant in that respect

even more chance of contam if you dont pasteurize your substrate

if you could successfully spawn cubes on non sterizlized spawn substrate, you should quit mycology, and try LOTTO or casino, cause your are one luck MF'er

Edited by tonyperez420 (07/28/06 07:45 AM)

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Invisiblecreamcorn
mad scientist
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Registered: 03/13/06
Posts: 2,962
Trusted Cultivator
Re: without sterilizing/pasteurizing [Re: tonyperez420]
    #5908548 - 07/28/06 10:34 AM (17 years, 8 months ago)

Quote:

tonyperez420 said:
maybe you can get away with it with your casing layer.... RogerRabbit boasts that he does not believe in sterilizing casings, but I always nuke my casings, so I am ignorant in that respect




yeah, he says not to sterilize, but its always followed by the suggestion to pasteurize.  its not a matter of his belief specifically, its conventional wisdom of many experienced growers.  pasteurization results in a large reduction in mold spore load, but allows some bacteria to survive.  the lower levels of bacteria that survive, help to keep things in check, and actually prevent it from becoming with mold contamination down the road, and some bacterial activity is actually useful for pin development.  there are some species (not cubes, but some edibles and others) that will not pin at all, unless there are certain bacteria present in the casing layer.  sterilizing would kill these, but they can find their way back in in the environment of your FC.  to go by the book, pasteurizing and NOT sterilizing it is proper.  don't misunderstand 'do not sterilize your casing' as 'don't do anything to your casing'

you can get away with using plain verm casing, out of the bag without treatment.

in fact, you CAN get away with any casing, without heat treatment, but its not optimal, and you're more or less lucky when it works.

depending on how you nuke, chances are you're getting the majority of your casing mix to paseurization temps anyway.  you're pastuerizing your casing and don't even realize that that's what you're doing. :smile:

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