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InvisibleSemilanceata
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Registered: 05/26/03
Posts: 841
Loc: República Federal Íbera
Macrolepiota procera
    #5905376 - 07/27/06 01:34 PM (17 years, 7 months ago)

Hi,

I´m germinating some spores on agar and get everytime the same kind of growth that don´t really know if it´s contamination or just that the myc. of this shroom is this way. Everytime I get contams on agar it looks like something (the mold) fighting against another thing (the myc.) but this looks more like one only thing.
Anyway here are some pics:





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Sr_Setahongo

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Offlineeris
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Re: Macrolepiota procera [Re: Semilanceata]
    #5905419 - 07/27/06 01:47 PM (17 years, 7 months ago)

Are they from wild spore prints/gill fragments?


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Immortal / Temporarily Retired
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InvisibleSemilanceata
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Registered: 05/26/03
Posts: 841
Loc: República Federal Íbera
Re: Macrolepiota procera [Re: eris]
    #5905455 - 07/27/06 02:00 PM (17 years, 7 months ago)

Spores are from a wild shroom printed last october.


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Sr_Setahongo

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Offlineeris
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Re: Macrolepiota procera [Re: Semilanceata]
    #5905478 - 07/27/06 02:07 PM (17 years, 7 months ago)

Cool, I used to try and culture a lot of my hunting finds.. never got around to growing most of them.

I don't know much about the cultivation of Lepiota, but I know some of the general agar techniques for isolation.

What are you planning to do with it?


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InvisibleSemilanceata
No god, no boss

Registered: 05/26/03
Posts: 841
Loc: República Federal Íbera
Re: Macrolepiota procera [Re: eris]
    #5905664 - 07/27/06 02:57 PM (17 years, 7 months ago)

Well, I plan to inoc.some wheat grain and use it as spawn for a substrate that I´m still thinking about. But that´s just my plan, the first thing I need to know if is that culture looks OK cause I never had a growth like that.


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Sr_Setahongo

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OfflineHotnuts
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Re: Macrolepiota procera [Re: Semilanceata]
    #5905912 - 07/27/06 04:16 PM (17 years, 7 months ago)

I'm not familiar with this mushroom either, but it wouldn't hurt to transfer some of the healthy looking sectors in that plate to new plates. Good luck none the less!

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Offlinefalcon
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Re: Macrolepiota procera [Re: Semilanceata]
    #5906199 - 07/27/06 05:56 PM (17 years, 7 months ago)

I started some L. procera from spores on agar last fall to see what it looks like.
The mycelium was similar to yours, white becoming light brown, I think mine was thicker though, and I don't remember the zoned growth that you have on your plate. I thought at the time it looks very much like A. bisporus
mycelium. I used PDYA.

What kind of agar are you using?

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InvisibleSemilanceata
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Registered: 05/26/03
Posts: 841
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Re: Macrolepiota procera [Re: falcon]
    #5907505 - 07/28/06 12:04 AM (17 years, 7 months ago)

The culture is a little bit old and is drying out that´s why it isn´t thick, but I have some more in the lab that looks the same way but thicker.
Agar is MYPA.


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Sr_Setahongo

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InvisibleCorporal Kielbasa

Registered: 05/29/04
Posts: 17,235
Re: Macrolepiota procera [Re: falcon]
    #5907629 - 07/28/06 12:48 AM (17 years, 7 months ago)

Quote:

falcon said:
I started some L. procera from spores on agar last fall to see what it looks like.
The mycelium was similar to yours, white becoming light brown, I think mine was thicker though, and I don't remember the zoned growth that you have on your plate. I thought at the time it looks very much like A. bisporus
mycelium. I used PDYA.

What kind of agar are you using?




maybe the different growth is do to enviroment? Maybe thin agar. All i know is each species of mushroom has its own way of growing. Some have a few ways............ But the arial and whispy radial growth does look to be of mushroom nature, not contam.

I say nock up some grain and stir up some mushroom love.....

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InvisibleSpeeker

Registered: 02/11/04
Posts: 881
Re: Macrolepiota procera [Re: Semilanceata]
    #5907697 - 07/28/06 01:15 AM (17 years, 7 months ago)

looks right to me.. zoned growth and all..

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InvisibleSemilanceata
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Registered: 05/26/03
Posts: 841
Loc: República Federal Íbera
Re: Macrolepiota procera [Re: Speeker]
    #5907738 - 07/28/06 01:43 AM (17 years, 7 months ago)

Thanks to everyone! :thumbup:

Let see how this turns out, as long as I learn something new is good for me.

Salu2.


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Sr_Setahongo

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