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 Semilanceata
No god, no boss
Registered: 05/26/03
Posts: 841
Loc: República Federal Íbera
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 Macrolepiota procera 
#5905376 - 07/27/06 03:34 PM (14 years, 5 months ago) |
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Hi,
I´m germinating some spores on agar and get everytime the same kind of growth that don´t really know if it´s contamination or just that the myc. of this shroom is this way. Everytime I get contams on agar it looks like something (the mold) fighting against another thing (the myc.) but this looks more like one only thing.
Anyway here are some pics:
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Sr_Setahongo
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 eris
underground
 
Registered: 11/18/98
Posts: 48,012
Loc: North East, USA
Last seen: 1 year, 6 months
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Are they from wild spore prints/gill fragments?
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Semilanceata
No god, no boss
Registered: 05/26/03
Posts: 841
Loc: República Federal Íbera
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Re: Macrolepiota procera [Re: eris]
#5905455 - 07/27/06 04:00 PM (14 years, 5 months ago) |
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Spores are from a wild shroom printed last october.
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Sr_Setahongo
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eris
underground
Registered: 11/18/98
Posts: 48,012
Loc: North East, USA
Last seen: 1 year, 6 months
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Cool, I used to try and culture a lot of my hunting finds.. never got around to growing most of them.
I don't know much about the cultivation of Lepiota, but I know some of the general agar techniques for isolation.
What are you planning to do with it?
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Semilanceata
No god, no boss
Registered: 05/26/03
Posts: 841
Loc: República Federal Íbera
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Re: Macrolepiota procera [Re: eris]
#5905664 - 07/27/06 04:57 PM (14 years, 5 months ago) |
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Well, I plan to inoc.some wheat grain and use it as spawn for a substrate that I´m still thinking about. But that´s just my plan, the first thing I need to know if is that culture looks OK cause I never had a growth like that.
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Sr_Setahongo
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Hotnuts
old hand
Registered: 02/26/05
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I'm not familiar with this mushroom either, but it wouldn't hurt to transfer some of the healthy looking sectors in that plate to new plates. Good luck none the less!
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falcon
In the green
Registered: 04/01/02
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I started some L. procera from spores on agar last fall to see what it looks like.
The mycelium was similar to yours, white becoming light brown, I think mine was thicker though, and I don't remember the zoned growth that you have on your plate. I thought at the time it looks very much like A. bisporus
mycelium. I used PDYA.
What kind of agar are you using?
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Semilanceata
No god, no boss
Registered: 05/26/03
Posts: 841
Loc: República Federal Íbera
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Re: Macrolepiota procera [Re: falcon]
#5907505 - 07/28/06 02:04 AM (14 years, 5 months ago) |
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The culture is a little bit old and is drying out that´s why it isn´t thick, but I have some more in the lab that looks the same way but thicker.
Agar is MYPA.
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Sr_Setahongo
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Corporal Kielbasa
Registered: 05/29/04
Posts: 17,229
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Re: Macrolepiota procera [Re: falcon]
#5907629 - 07/28/06 02:48 AM (14 years, 5 months ago) |
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Quote:
falcon said:
I started some L. procera from spores on agar last fall to see what it looks like.
The mycelium was similar to yours, white becoming light brown, I think mine was thicker though, and I don't remember the zoned growth that you have on your plate. I thought at the time it looks very much like A. bisporus
mycelium. I used PDYA.
What kind of agar are you using?
maybe the different growth is do to enviroment? Maybe thin agar. All i know is each species of mushroom has its own way of growing. Some have a few ways............ But the arial and whispy radial growth does look to be of mushroom nature, not contam.
I say nock up some grain and stir up some mushroom love.....
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Speeker
Registered: 02/11/04
Posts: 759
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looks right to me.. zoned growth and all..
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Semilanceata
No god, no boss
Registered: 05/26/03
Posts: 841
Loc: República Federal Íbera
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Re: Macrolepiota procera [Re: Speeker]
#5907738 - 07/28/06 03:43 AM (14 years, 5 months ago) |
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Thanks to everyone! 
Let see how this turns out, as long as I learn something new is good for me.
Salu2.
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Sr_Setahongo
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