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Offlinedeleriance
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Spore clumps won't unclump
    #590319 - 03/26/02 08:53 PM (15 years, 2 days ago)

SWIM recently received some Thai LY syringed from The Hawk's Eye. They have the characteristic black spore clumps, which is, of course, no problem. However, when I shake the syringes (and I've shaken very vigorously), the clumps of spores do not break up whatsoever. Instead they simply shake around, then settle back to the bottom. This isn't supposed to be what happens, is it? To me, it seems that it would be incredibily difficult to perform uniform innoculation with such a syringe. Help, please?


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Offlinebreathingofmist
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Re: Spore clumps won't unclump [Re: deleriance]
    #590336 - 03/26/02 09:09 PM (15 years, 2 days ago)

Suggestion, if you havn't already. Suck in some sterile air..it MAY help.



Hope this helps



-smoke on the water, fire in the sky-


--------------------


"Breathing of mist, soul enshrouded, I am just... an Illusion"


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Offlinenorthwood
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Re: Spore clumps won't unclump [Re: breathingofmist]
    #590384 - 03/26/02 10:14 PM (15 years, 2 days ago)

i have had this happen too, generally there is still pleanty of spores in the water but the clumps get very anoying when they get stuck in the needle and you have to force them out and end up spraying like 5cc of good water all over


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InvisibleWakingUpLate
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Re: Spore clumps won't unclump [Re: deleriance]
    #590399 - 03/26/02 10:34 PM (15 years, 2 days ago)

Take a shot glass and clean it up good with alcohol and
let it dry. Then squirt the syringe into it and draw it back
up a couple of times. The water will be purple again.:peace:
Free Spore Ring

 


--------------------
The rest of those, who have gone before us,
cannot settle the unrest of those who follow.
(Finding Forrester)


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Anonymous

Re: Spore clumps won't unclump [Re: deleriance]
    #590785 - 03/27/02 07:27 AM (15 years, 1 day ago)

Sometimes they just will not dissolve but if you have big clumps in your syringe, you can be guaranteed there are many other spores floating around that you can't see.

The jury is still out but I believe older, drier prints are more prone to clumpage. I've just made some syringes from fresh prints and noticed no clumpage at all, just a tinge in the water.

So on one hand, the spores are likely a few weeks or months older - shouldn't make too much diff - but they also guarantee esaier spore mating when they are already clumped together.

Then again, I could be wrong....



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Offlinethe_alchemist
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Re: Spore clumps won't unclump [Re: Anonymous]
    #10483605 - 06/10/09 07:08 PM (7 years, 9 months ago)

totally agree with u clumping gets more evident with the aging of spores i have noticed that syringes made with 3 or 4 days spores the water is very purple, one year old spores get ready for clumping
best solution could be as referred above one drop of natural dish washer in 1 pint of sterile distillate water to prevent clumping never tried myself but will start from now


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experiment_and free your mind


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Offlinejohnwayne
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Re: Spore clumps won't unclump [Re: the_alchemist]
    #10483616 - 06/10/09 07:11 PM (7 years, 9 months ago)

thats weird, sporeowrk was out of pe, as soon as they were back online i order a 4pack, they all came with great big clumps, and are working great. i got some syringes without clumping, just a homogenous mixture and they sucked balls


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Offlineveda_sticks
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Re: Spore clumps won't unclump [Re: johnwayne]
    #10483676 - 06/10/09 07:24 PM (7 years, 9 months ago)

Spore clumping will happen over time. Little black specs floating around is fine. If its large clumps that's not right. I've only ever ecperienced that kind of clumping from a print that wasn't allowed to dry which makes the spores stick together and refuse to break up properly when hydrated.

Maybe they don't use jet dry in there syringe or maybe its just been sitting formonths.


Syringes should not be purple. Purpe = far too many spores. Dilute those syringes untill they are clear


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Offlinethe_alchemist
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Re: Spore clumps won't unclump [Re: veda_sticks]
    #10485187 - 06/10/09 11:28 PM (7 years, 9 months ago)

massively purple syringes have worked 4 me u say that in the waste sense or too many spores may compete one one another or what? till this day i thought-still do that the more spores the quicker...feel free to reply I'm here to learn, it takes a whole life and most of the times it is not enough even so


--------------------
experiment_and free your mind


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OfflineRogerRabbitM
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Re: Spore clumps won't unclump [Re: the_alchemist]
    #10485199 - 06/10/09 11:29 PM (7 years, 9 months ago)

Lay the syringe in an ultrasonic jewelry cleaner for a minute or two.
RR


--------------------
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Offlinethe_alchemist
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Re: Spore clumps won't unclump [Re: RogerRabbit]
    #10485323 - 06/10/09 11:56 PM (7 years, 9 months ago)

now that's a hot tip throw i have no idea where i get to use one of those but haven't Even thought of it wonderful forums


--------------------
experiment_and free your mind


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Invisiblegeorge castanzaM
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Re: Spore clumps won't unclump [Re: the_alchemist]
    #10485366 - 06/11/09 12:05 AM (7 years, 9 months ago)

any reason you are bumping 7 year old threads?


--------------------



     
Quote:

pftek said:Impossible to clean everything right now. Maybe in a week.





ROTHBURY

:pm:


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Offlinethe_alchemist
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Re: Spore clumps won't unclump [Re: george castanza]
    #10485380 - 06/11/09 12:09 AM (7 years, 9 months ago)

sory not realy iv been groing for the past 5 years posting inly now i guess u could call it 1st posts fever...lol ill stop hihi


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experiment_and free your mind


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Invisiblegeorge castanzaM
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Re: Spore clumps won't unclump [Re: the_alchemist]
    #10485393 - 06/11/09 12:12 AM (7 years, 9 months ago)

post fever, na, we call it post whoring, it seems to get everyone around here from time to time. :gethigh:


--------------------



     
Quote:

pftek said:Impossible to clean everything right now. Maybe in a week.





ROTHBURY

:pm:


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OfflinePsuper
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Re: Spore clumps won't unclump [Re: the_alchemist]
    #10485410 - 06/11/09 12:16 AM (7 years, 9 months ago)

Yeah, old thread, but while we're on the subject, does anyone remember Magash's tek involving putting a couple drops of Jet-Dry in the water? lol I haven't heard of anyone doing that for awhile.

If you got some big clumps, make that into two or three syringes.  Leave a little air in the syringe to shake. ~Pixie~


--------------------
P. Cubensis Bulk Substrate:
Coco-Coir (and/or local Horse Manure), Vermiculite, Gypsum (calcium sulfate), Composted Chicken Manure (3%-5%).


Visit the Growery.Org, Here's a thread by myself about water-hash bags and hashish filtration:
http://www.growery.org/forums/showflat.php/Number/489978


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Invisiblemango man
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Re: Spore clumps won't unclump [Re: RogerRabbit]
    #10485435 - 06/11/09 12:22 AM (7 years, 9 months ago)

like for cleaning the ink out of a tattoo gun?


--------------------
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I'm looking for edibles and prints from woodlovers.
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InvisibleBlimeyGrimey
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Re: Spore clumps won't unclump [Re: Psuper]
    #10485443 - 06/11/09 12:24 AM (7 years, 9 months ago)

Quote:

Psuper said:
Yeah, old thread, but while we're on the subject, does anyone remember Magash's tek involving putting a couple drops of Jet-Dry in the water? lol I haven't heard of anyone doing that for awhile.





I used to use Jet-Dry when I made syringes. It really does help keep clumps to a minimum and doesn't harm the spores at all.

Also, spore clumping happens due to spores germinating in the syringe. That's why some syringes have that blob with specks in them. Germinating spore will and do cannibalize other spores, thus they will grow in plain distilled water.


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Invisiblegeorge castanzaM
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Re: Spore clumps won't unclump [Re: Psuper]
    #10486893 - 06/11/09 09:43 AM (7 years, 9 months ago)

Quote:

Psuper said:
Yeah, old thread, but while we're on the subject, does anyone remember Magash's tek involving putting a couple drops of Jet-Dry in the water? lol I haven't heard of anyone doing that for awhile.

If you got some big clumps, make that into two or three syringes.  Leave a little air in the syringe to shake. ~Pixie~




I think that a lot of folk's are doing it, just not that much conversation about it anymore...
Not sure, but I think that a lot of vendors use this tek.  I never do, but I am lazy.  Extra step, not essential, will not improve yield, skip it(that is just how I roll).  It is a good tek though.


--------------------



     
Quote:

pftek said:Impossible to clean everything right now. Maybe in a week.





ROTHBURY

:pm:


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Offlineveda_sticks
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Re: Spore clumps won't unclump [Re: the_alchemist]
    #10486953 - 06/11/09 10:11 AM (7 years, 9 months ago)

Quote:

the_alchemist said:
massively purple syringes have worked 4 me u say that in the waste sense or too many spores may compete one one another or what? till this day i thought-still do that the more spores the quicker...feel free to reply I'm here to learn, it takes a whole life and most of the times it is not enough even so





No they wont compete, its a waste of spores and can be counter productive.

You may see faster growth, but its not growing faster at all. What you are seeing is a much larger number of germinated spores growing at once. All those mycelial networks then have to join together wasting energy and time. I recieved a very nice syringe from hoodbran that was very clear, it had a few specs floating around. Saw growth start at day 3, and there colinising very nicely. without incubation. just in my livingroom probably between 70-75F

The more spores you use, the more chance you have of having incompatible networks that wont combine. They wont compete, 1 wont win, they will simply grow side by side without the benifit of the full substrate.

Try making your spore syringes not as loaded. And see the difference. Prints will go further too.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


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