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Offlinejollyjingles
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Registered: 07/26/06
Posts: 4
Last seen: 15 years, 10 months
1 out of 4 no growth
    #5902591 - 07/26/06 06:26 PM (17 years, 6 months ago)

We inoculated four jars of substrate 12 days ago. Two of the jars are doing very well, one is slower but still steady, our fourth jar never showed signs of growth. We reinoculated the one jar 6 days ago to see if it would produce as the others have. All four jars were inoculated at the same temperature and using the same syringe. Any ideas as to why jar four has no growth what so ever? Just curious.


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Offlinejollyjingles
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Registered: 07/26/06
Posts: 4
Last seen: 15 years, 10 months
Re: 1 out of 4 no growth [Re: jollyjingles]
    #5906900 - 07/27/06 09:06 PM (17 years, 6 months ago)

I guess everyone is as clueless as we are to this subject!!


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OfflineEquilibriuM
dream stalker

Registered: 07/17/05
Posts: 2,323
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Re: 1 out of 4 no growth [Re: jollyjingles]
    #5906907 - 07/27/06 09:07 PM (17 years, 6 months ago)

bacteria


--------------------
HELP!!!!!!!!!


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Invisiblecreamcorn
mad scientist
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Registered: 03/13/06
Posts: 2,962
Trusted Cultivator
Re: 1 out of 4 no growth [Re: EquilibriuM]
    #5906925 - 07/27/06 09:12 PM (17 years, 6 months ago)

Quote:

EquilibriuM said:
bacteria




very likely. and why you should give up on non-growing jars. because if it is bacteria, and you stuck your syringe in there, bad news.


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OfflineWeebl8bob
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Re: 1 out of 4 no growth [Re: creamcorn]
    #5907266 - 07/27/06 10:53 PM (17 years, 6 months ago)

What if.. your jars did this and you used more LC and myc started growing?


--------------------
"You can turn your back on a person, but never turn your back on a drug, especially when its waving a razor sharp hunting knife in your eye." - Hunter S. Thompson


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OfflineYamidude
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Registered: 06/15/06
Posts: 957
Last seen: 15 years, 5 months
Re: 1 out of 4 no growth [Re: Weebl8bob]
    #5907309 - 07/27/06 11:05 PM (17 years, 6 months ago)

Quote:

Weebl8bob said:
What if.. your jars did this and you used more LC and myc started growing?


WHAT IF, you just made some new jars and innocced them with the LC you have instead of wasting your time and playing what if?


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OfflineComfortablyStond
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Registered: 03/15/06
Posts: 239
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Re: 1 out of 4 no growth [Re: Yamidude]
    #5907788 - 07/28/06 02:05 AM (17 years, 6 months ago)

I played the "what if" game with a PF jar shortly after my first grow. It never showed signs of growth or of what I would have then thought to be contamination. I shot it up with some LC of the same strain originally used, and incubated. 4 days later, there was HEAVY myc growth, and we did the happy jar dance. 3 days later, that's 7 days days after the 2nd insemination, FUCKIN' AWFUL BLUISH GREEN FUNKY NASTY MOLD EVERYWHERE!!!!!!!!!! Don't bother with it man...remake the jar, rePC, and reinject. Snot really worth the wasted time, high hopes, and shatterred dreams, crushed soul, and obliterated spirit of watching beautiful white become the funk-nasty.

Peace.


--------------------
"It's not a war on drugs. It's a war on personal freedom. Keep that in mind at all times..." Bill Hicks


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Offlinejollyjingles
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Re: 1 out of 4 no growth [Re: ComfortablyStond]
    #5908451 - 07/28/06 09:48 AM (17 years, 6 months ago)

We've already got three good ones.. that was the last jar we inoculated.  Not really worried about it just curious :smile:


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Offlinedot_com_unist
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Registered: 07/02/06
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Re: 1 out of 4 no growth [Re: jollyjingles]
    #5908473 - 07/28/06 10:01 AM (17 years, 6 months ago)

did you shake your syringe well before inoculating?
it is possible that you shot all of your clusters into those jars and just injected water into the fourth. Just a thought... Make sure you shake well prior to and in between inocs.


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Offlinejollyjingles
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Re: 1 out of 4 no growth [Re: dot_com_unist]
    #5908715 - 07/28/06 11:39 AM (17 years, 6 months ago)

Yeah it was shook and mixed well. The second inoculation had a big ol' chunk of spores. But we're not giving up on it. Just keeping it around but do not really expect much!


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OfflineWeebl8bob
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Re: 1 out of 4 no growth [Re: Yamidude]
    #5909715 - 07/28/06 06:13 PM (17 years, 6 months ago)

Uhh... this is my second batch of jars that have done this to me...


--------------------
"You can turn your back on a person, but never turn your back on a drug, especially when its waving a razor sharp hunting knife in your eye." - Hunter S. Thompson


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Invisiblemonstermitch
Growing in Bags Doesn't Work

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Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay Flag
Re: 1 out of 4 no growth [Re: Weebl8bob]
    #5909724 - 07/28/06 06:15 PM (17 years, 6 months ago)

^^

they didn't do it to you.
you did it to them.

like a poorly trained dog...barking, jumping...
the owners fault.

never repeat the same behavior expecting a different result


--------------------



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Offlinenoleshroomer
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Registered: 07/10/06
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Re: 1 out of 4 no growth [Re: monstermitch]
    #5909784 - 07/28/06 06:57 PM (17 years, 6 months ago)

Where did you get the syringe.. maybe it was a very impotent syringe and only a tiny water with a very small amount of spores went in the last one.. If thats true it and your jars have verm on top maybe it will grow fine just take forever because its growing from such a small amount of spores.


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Offlinedot_com_unist
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Re: 1 out of 4 no growth [Re: monstermitch]
    #5909937 - 07/28/06 08:12 PM (17 years, 6 months ago)

Quote:

monstermitch said:
^^

they didn't do it to you.
you did it to them.

like a poorly trained dog...barking, jumping...


the owners fault.

never repeat the same behavior expecting a different result



:grin: LOL!


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Offlinedot_com_unist
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Re: 1 out of 4 no growth [Re: dot_com_unist]
    #5909947 - 07/28/06 08:15 PM (17 years, 6 months ago)

My guess would be poor spores, you cooked em, or they dried out. I think it is the spores though. You will not always get every jar unless you are doing your own prints/syringe or your getting dark syringes from a vendor which I doubt.


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OfflineWeebl8bob
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Re: 1 out of 4 no growth [Re: monstermitch]
    #5910663 - 07/28/06 11:42 PM (17 years, 6 months ago)

Quote:

monstermitch said:
^^

they didn't do it to you.
you did it to them.

like a poorly trained dog...barking, jumping...
the owners fault.

never repeat the same behavior expecting a different result




Good point.. so your saying do the same thing I did to the last batch to get em working? ... I just knocked em up 3 times and they finally started getting noticable myc


--------------------
"You can turn your back on a person, but never turn your back on a drug, especially when its waving a razor sharp hunting knife in your eye." - Hunter S. Thompson


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Offlinehawksapprentice
Yearns to Snowboard
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Registered: 06/06/03
Posts: 3,195
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Re: 1 out of 4 no growth [Re: Weebl8bob]
    #5910938 - 07/29/06 01:44 AM (17 years, 6 months ago)

That's ridiculous, and a waste of spores.

If your doing things right you don't need to inoculate 3 times to see growth.


--------------------
"I celebrate the Earth, my home, my mother, my grave, and as long as men are Man they must, if they would preserve the integrated being, do the same---[and preserve]--this rank casual hungry smelly sweaty lusting transitory body, my oozy pulpy liquid-bag-swollen body, bones, blood, hair glands, my bejeweled sex; I love and celebrate it all.  never to let men forget that they are animals as much as gods---that is one thing I shall say."

  Edward Abbey


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