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Offlinepsyche_sailor
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incubation & drying
    #5901810 - 07/26/06 02:26 PM (14 years, 9 months ago)

im about to case my crumbled cakes in a vermiculite casing, how long should i cover them for? with holes/without?

i like to keep things simple so i think im going to just use a sealed transparent box with perlite in the bottom - what do you reckon?

also whats the best drying method without spending silly amounts...

peace


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InvisibleAtheist
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Re: incubation & drying [Re: psyche_sailor]
    #5901816 - 07/26/06 02:28 PM (14 years, 9 months ago)

when i did pf i wrapped the casings with saran wrap, no holes

placed in incubator for a little more than 48 hours, just till myc showed through

patch then fruit

buy a dehydrator, you can always return it when your done :thumbup:


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Offlinepsyche_sailor
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Re: incubation & drying [Re: Atheist]
    #5901850 - 07/26/06 02:51 PM (14 years, 9 months ago)

the dehydrator is too much hassle for me, any other advice from you expert mushroomen and women


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Re: incubation & drying [Re: psyche_sailor]
    #5901901 - 07/26/06 03:12 PM (14 years, 9 months ago)

fan dry for 2 days

then desiccant chamber

costs less than 5 bucks


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InvisibleAtheist
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Re: incubation & drying [Re: Atheist]
    #5901902 - 07/26/06 03:12 PM (14 years, 9 months ago)

or see if you can borrow a dehydrator

many people have one buried in the kitchen or garage


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Offlinemyclops
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Re: incubation & drying [Re: Atheist]
    #5902029 - 07/26/06 04:16 PM (14 years, 9 months ago)

cover casings and incubate for about 2-3 days before checking. as soon as mycelium is showing in the low parts of the casing evenly, patch and put in fruiting chamber. i use a translucent plastic container with perlite and have no problem keeping 95% Rh. Fan dry for a day or two and finish up with Damp Rid until cracker dry. very inexpensive and reusable.


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OfflineYamidude
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Re: incubation & drying [Re: myclops]
    #5902354 - 07/26/06 06:37 PM (14 years, 9 months ago)

my dehydrator gets up to 150 degrees which i don't like. im at a loss. i guess i may be fan drying..


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Offlinepsyche_sailor
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Re: incubation & drying [Re: Yamidude]
    #5904173 - 07/27/06 03:50 AM (14 years, 9 months ago)

im using a foil baking dish for casing about 8'' by 4''. what depth/ratio substrate to casing do you recommend?


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OfflineSinthetic
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Re: incubation & drying [Re: psyche_sailor]
    #5904460 - 07/27/06 08:03 AM (14 years, 9 months ago)

Mushrooms are fine up to psilocybin is stable up to 400 degrees F.


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Re: incubation & drying [Re: Sinthetic]
    #5904469 - 07/27/06 08:09 AM (14 years, 9 months ago)

Don't throw out bullshit numbers like that. Psilocybin isn't even completely "stable" at room temp. At 400F it's not going to last more than an hour at best.


-FF


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Re: incubation & drying [Re: fastfred]
    #5904492 - 07/27/06 08:38 AM (14 years, 9 months ago)

No BS guy. Sorry you don't like it. It's a fact. "Psilocybin is heat stable and can be extracted unchanged from psilocybin/psilocin-containing mushrooms.
http://www.urmc.rochester.edu/URMC/AAPCC/mushrooms2/discussion3.htm

What makes you think psilocybin is unstable at room temp. "Psilocybin is highly stable, and it has been reported that psilocybin can last for an extremely long amount time and was even detected in a hundred fifteen year old mushroom sample"
http://sulcus.berkeley.edu/mcb/165_001/papers/manuscripts/_471.html

Don't accuse people of giving misinformation when you're the one spreading old wive's tales.


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Offlinepsyche_sailor
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Re: incubation & drying [Re: Sinthetic]
    #5904528 - 07/27/06 09:13 AM (14 years, 9 months ago)

uum yeah so how deep should the substrate be..


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Re: incubation & drying [Re: Sinthetic]
    #5904538 - 07/27/06 09:24 AM (14 years, 9 months ago)

Psilocybin is not "highly stable", it is relatively stable when compared to *unstable* compounds. It is easily dephosphorylated by protonation in an acidic environment. It is then easily oxidized by oxygen or any proton acceptor.

Psilocybin will be significantly degraded over the course of a year storage at room temp. I don't have the citation, but psilocybin stability is significantly enhanced by cold storage.

That's not a wives tale, it's what's been reported in the literature.


-FF


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Re: incubation & drying [Re: fastfred]
    #5904559 - 07/27/06 09:40 AM (14 years, 9 months ago)

Now you're changing your story and making it subjective. I don't want to argue with you. The point is you can dry your shrooms in a dehydrator at 150F with out losing more that 5% potency. Last time I checked that wasn't an "acidic environment".

Psyche, sorry about halfway hijacking your thread. If you have enough sub and room in the tray I would go with 4" deep sub and 1/2" deep casing.


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Re: incubation & drying [Re: Sinthetic]
    #5924601 - 08/02/06 08:58 AM (14 years, 9 months ago)

"A second important hallucinogenic drug — psilocybin — belongs to very polar and thermal labile substances and is after ingestion (or after injection into GC) immediately dephosphorylated to psychoactive psilocin."

Journal of Chromatography B: Biomedical Sciences and Applications
Volume 748, Issue 1 , 1 October 2000, Pages 3-19


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Re: incubation & drying [Re: fastfred]
    #5924605 - 08/02/06 09:01 AM (14 years, 9 months ago)

So are you trying to say that a food dehydrator can dephosphorylate?


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Re: incubation & drying [Re: Sinthetic]
    #5924642 - 08/02/06 09:29 AM (14 years, 9 months ago)

There are enzymes in the fruit bodies that will do the dephosphorylation, and it is significantly increased by heating. Any acidic environment will also cause dephosphorylation, and the rate of this is also increased by heat. So unless your substrate is pH'ed with some buffer to keep it basic you will probably suffer from both enzymatic and acidic dephosphorylation.

After psilocybin is dephosphorylated to psilocin it's quite unstable and quickly oxidized or degraded by heat.


-FF


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