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whatsthestorymg
badwithgrains

Registered: 07/23/06
Posts: 123
Last seen: 16 years, 10 months
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Liquid Culture Attempt
#5895192 - 07/24/06 06:25 PM (17 years, 6 months ago) |
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Well i decided to try to do my first liquid culture today based on a tek i found on here about simple liquid culture.. I ordered some cubie spores, equadorian strain. Their shape intrigues me and i've wanted to try them for awhile. The Tek i used was here. <a href="/forums/showflat.php/Cat/0/Number/5238137/page/0/fpart/1/vc/1/nt/14"> A *beginner friendly* method for making Liquid Culture</a>
So i boiled the jars in my trusty dutch oven with the grate for 30 minutes.. punched lid holes, made the boiling water/1 tablespoon of honey mix, capped, foiled, and boiled for an additional 40 minutes.
The question that i have is.. I look at my jars, and there are particulates in them that i suspect are just from out nasty water system. We tend to get a lot of crud in our city water. We have two filters running into our refrigerator/ice maker. Will these particulates hinder the growth of the mycelium in the jars? i don't want to waste a whole syringe of spores to find out that they stopped growth. I'm guessing they shouldn't since they were sterilized for 70 minutes but i'd like some input from others.
maybe i'll just do two of my jars LC, and make the other two cake style just so this whole thing doesn't become a total loss.
-------------------- I pity the fool who don't enjoy a good cubie I pity the fool who don't enjoy a good van eyck My Unitarian Jihad Name is: Brother Cattle Prod of Loving Kindness
Edited by whatsthestorymg (07/24/06 06:27 PM)
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Yamidude
Stranger

Registered: 06/15/06
Posts: 957
Last seen: 15 years, 5 months
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honey can sometimes show particulates. I made some with honey and some with Karo the other day. the honey ones look all funky. The Karo ones are showing growth already and looking good.
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Slainhobo
Stranger

Registered: 07/02/06
Posts: 7
Last seen: 16 years, 10 months
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Re: Liquid Culture Attempt [Re: Yamidude]
#5895250 - 07/24/06 06:47 PM (17 years, 6 months ago) |
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Quote:
Yamidude said: honey can sometimes show particulates. I made some with honey and some with Karo the other day. the honey ones look all funky. The Karo ones are showing growth already and looking good.
So you could replace the honey with the same amount of the Karo and use the same steps? I wish I knew this this morning when I made four LCs.
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MustardMan
Peace Frog


Registered: 10/18/05
Posts: 970
Last seen: 5 years, 5 months
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Re: Liquid Culture Attempt [Re: Slainhobo]
#5895279 - 07/24/06 06:57 PM (17 years, 6 months ago) |
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A lot of honey will often have sediment and tiny hair like dust in it, don't worry about it, but it is nice to have a clear clean cloud of pure Myc...
Remember, its ok if your solution is a little less than 4g:100ml water, but do not exceed 4g per 100ml of water... The LC should be almost clear.
> I like to use my scale when putting in the honey, usually 8grams of honey, and about 225 ml of water per glass (you won't be able to suck up honey in a syringe). Also, if your PCing your LC for 70 minutes, thats way to long... Even if your using just a pot with a rag on the bottom, 25 minutes should work fine...
-------------------- Wild Psilocybe Ovoideocystidiata
 Cultivated Cubensis

Edited by MustardMan (07/24/06 07:04 PM)
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baycafe
Urawa RedDiamonds


Registered: 09/11/05
Posts: 500
Loc: 埼玉県&...
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Re: Liquid Culture Attempt [Re: MustardMan]
#5895292 - 07/24/06 07:01 PM (17 years, 6 months ago) |
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It is easy to remove the particulates from a honey LC. Just sieve the honey into your jar via a coffee filter and scalding hot water. All the wax and impurities say in the filter leaving you with a nice clear solution for growth of your favorite gourmet cultures. Use the same ratio. It works well and is cheap enough to make.
-------------------- I think my eyes are getting better. Instead of a big dark blur I see a big light blur. 俺のシロシャイビケッルリプスがここです。 東京スカパラダイスオクストラ
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whatsthestorymg
badwithgrains

Registered: 07/23/06
Posts: 123
Last seen: 16 years, 10 months
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Re: Liquid Culture Attempt [Re: baycafe]
#5895465 - 07/24/06 07:52 PM (17 years, 6 months ago) |
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That's 70 minutes total.. 40 minutes just boiling the jar and lid, then 30 minutes boiling the honey/water mixture. Other question... I had read somewhere that too much water in a BRF cake will "drown" mycelium? how does the liquid culture differ from this..? hmm. I'm hesitant about using good spore solution for this. Wasting spores is among the most heinous of offenses. Maybe i'll try it with one jar, and then if it works proceed with the inoculation of the rest 
I am nervous about trying this
Edited by whatsthestorymg (07/24/06 07:55 PM)
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creamcorn
mad scientist


Registered: 03/13/06
Posts: 2,962
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mycelium digests food by excreting enzymes which breaks down said food into mainly simple sugars. too much water in a brf cake means as those enzymes are excreted and diluted, rendering them much less effective. its kind of like if we pulled all your teeth out and gave you a nice juicy steak. (or some tofu hippy nonsense if you're one of those vegetarians haha)
in a liquid culture, they're surrounded by every angle with simple sugars. they don't have to really do any digesting, its ready to go. going with the same sorta analogy, its like hooking up a feeding tube to your viens. 
like you said at first, make a few cakes, make some lc. if the cakes fail and the lc works, you got lc now to try the cakes again. if the cakes work but the lc fails, you got mushrooms, can take spore prints, and have more spores than you know what to do with so you won't be so miserly with them in the future. if both work, well fantastic
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liamtheloser
Advanced Idiot

Registered: 06/07/06
Posts: 1,453
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too much water in a brf jar might not drown mycellium, but it will definately slow growth, and provides a great breeding ground for 'wetspot' and other bacteria.
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MustardMan
Peace Frog


Registered: 10/18/05
Posts: 970
Last seen: 5 years, 5 months
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I don't know why the Myc doesn't drown in LC but will if under water for like 48 hours (on a cake)... Good question- but LC myc won't drown, don't worry.
If spore solution is very precious to you, then LC IS FOR YOU!
Think about it, make one jar of 200ML Liquid culture, you can use as little as 1/2 a CC to inoculate if you wish, it will just take less time with more spores.
After you have 200ml of LC,(200 CC's! A syringe only holds like 10-12CC's)you can then use that LC to inoculate other LC's, and those will colonize even faster.
This beautiful cycle is why everyone loves LC; not to mention its very fast at colonizing jars.
-------------------- Wild Psilocybe Ovoideocystidiata
 Cultivated Cubensis

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whatsthestorymg
badwithgrains

Registered: 07/23/06
Posts: 123
Last seen: 16 years, 10 months
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Re: Liquid Culture Attempt [Re: MustardMan]
#5895691 - 07/24/06 08:42 PM (17 years, 6 months ago) |
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just wonder.. this is totally noobish.. what's a comparison of colonization time? my jars take three weeks to colonize, an LC jar would take?.. just ballpark.. i know there's gonna be differences based on strain, methods, conditions.
-------------------- I pity the fool who don't enjoy a good cubie I pity the fool who don't enjoy a good van eyck My Unitarian Jihad Name is: Brother Cattle Prod of Loving Kindness
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creamcorn
mad scientist


Registered: 03/13/06
Posts: 2,962
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typically for me:
spores -> lc = 7-10 days lc -> lc = 3-4 days spores -> cake = 13-16 days lc -> cake = 9-12 days
(half pint cakes)
lc -> wbs = 7-10 days spores -> wbs = 9-12 days
(quarts of wbs)
basically, when innoculating something from LC, you shave off the time it takes for the spores to germinate. which is 2-3 days on the average.
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liamtheloser
Advanced Idiot

Registered: 06/07/06
Posts: 1,453
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a week?
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whatsthestorymg
badwithgrains

Registered: 07/23/06
Posts: 123
Last seen: 16 years, 10 months
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So if im reading all this right.. with an LC you can inoculate countless more jars than with a spore syringe? hmm... Err I don't like boiling my jars.. that's the only thing. making jars is a hassle.. TO THE THRIFT STORE! i need to find a pressure cooker. My BRF jars are coming along nicely.. i turned them upside down and colonization on the bottoms are coming along nicely. heehee. There's condensation.
-------------------- I pity the fool who don't enjoy a good cubie I pity the fool who don't enjoy a good van eyck My Unitarian Jihad Name is: Brother Cattle Prod of Loving Kindness
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creamcorn
mad scientist


Registered: 03/13/06
Posts: 2,962
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thats the point. why else did you think we make them? better yet, why were you trying to make one without even knowing what it was for?
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Yamidude
Stranger

Registered: 06/15/06
Posts: 957
Last seen: 15 years, 5 months
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Re: Liquid Culture Attempt [Re: creamcorn]
#5896172 - 07/24/06 10:25 PM (17 years, 6 months ago) |
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Both my karo LC jars with TX strain are showing good growth. Im pumped. Im going to buy a box of quart jars later this week to start some fresh ones.
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whatsthestorymg
badwithgrains

Registered: 07/23/06
Posts: 123
Last seen: 16 years, 10 months
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Re: Liquid Culture Attempt [Re: Yamidude]
#5896218 - 07/24/06 10:34 PM (17 years, 6 months ago) |
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I knew it sped up colonization time. That was my motivation to try it! gosh.. haha. Let me know how the texas strain is. I've been meaning to try and start some, because the strain is native to my homeland. Hah, but i haven't heard much about them.
-------------------- I pity the fool who don't enjoy a good cubie I pity the fool who don't enjoy a good van eyck My Unitarian Jihad Name is: Brother Cattle Prod of Loving Kindness
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Yamidude
Stranger

Registered: 06/15/06
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Last seen: 15 years, 5 months
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I like the idea of LC because if you wanted to you could suck some up and inject it into a gallon jar of LC solution and have ungodly amounts of LC all from an initial 3-4cc's of spore syringe.
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whatsthestorymg
badwithgrains

Registered: 07/23/06
Posts: 123
Last seen: 16 years, 10 months
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Re: Liquid Culture Attempt [Re: Yamidude]
#5896395 - 07/24/06 11:30 PM (17 years, 6 months ago) |
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will lc store well? - on other topics.. just made some more jars.. put them in the fridge going to sterilize them tomorrow.. hopefully the fridge will hold off bacterial growth till i can get the pot set up tomorrow. I have to be at work at 10 so im going to bed... : :
-------------------- I pity the fool who don't enjoy a good cubie I pity the fool who don't enjoy a good van eyck My Unitarian Jihad Name is: Brother Cattle Prod of Loving Kindness
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liamtheloser
Advanced Idiot

Registered: 06/07/06
Posts: 1,453
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lc stores great, just keep them in the fridge and they'll keep for at least a few months... I've seen someone say 6+ months in fridge.
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whatsthestorymg
badwithgrains

Registered: 07/23/06
Posts: 123
Last seen: 16 years, 10 months
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I've got a question about my liquid cultures. I see little stringy chunks of something. Are these the mycelium? I inoculated them on friday. The fact that they are brown bothers me but i think they're just brown because the honey makes them look that way... eep.
-------------------- I pity the fool who don't enjoy a good cubie I pity the fool who don't enjoy a good van eyck My Unitarian Jihad Name is: Brother Cattle Prod of Loving Kindness
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Tippinthru
contented

Registered: 04/07/05
Posts: 1,131
Loc: "The Garden"...
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If they look like above, .
You may have also caramelized sugars, in your LC. So, sometimes myc is a bit browner, tainted by the LC color.
-------------------- Perfection is attained by slow degrees; it requires the hand of time... [
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whatsthestorymg
badwithgrains

Registered: 07/23/06
Posts: 123
Last seen: 16 years, 10 months
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Re: Liquid Culture Attempt [Re: Tippinthru]
#5917010 - 07/30/06 11:21 PM (17 years, 6 months ago) |
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So they are okay to use if brown? mine doesn't have quite that much in it yet. I'm only 2 1/2 days into it. But soon!
-------------------- I pity the fool who don't enjoy a good cubie I pity the fool who don't enjoy a good van eyck My Unitarian Jihad Name is: Brother Cattle Prod of Loving Kindness
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treeyei
spork


Registered: 05/14/05
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how's it going whatsthestorymg?
-------------------- Everything I post (or have posted) on this website is fictional, even this sentence.
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whatsthestorymg
badwithgrains

Registered: 07/23/06
Posts: 123
Last seen: 16 years, 10 months
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Re: Liquid Culture Attempt [Re: treeyei]
#5928766 - 08/03/06 02:33 PM (17 years, 5 months ago) |
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pretty good.. i got some nice fuzzy clouds, and some clumpy rhizomorphs that are awesome to look at. I think they should be done by tomorrow. Water almost totally clear.
-------------------- I pity the fool who don't enjoy a good cubie I pity the fool who don't enjoy a good van eyck My Unitarian Jihad Name is: Brother Cattle Prod of Loving Kindness
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Lily_Morgan
I'm #1 !!

Registered: 07/05/06
Posts: 437
Loc: Eastern Shore
Last seen: 16 years, 3 months
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Quote:
whatsthestorymg said: I knew it sped up colonization time. That was my motivation to try it! gosh.. haha. Let me know how the texas strain is. I've been meaning to try and start some, because the strain is native to my homeland. Hah, but i haven't heard much about them.
Texas colonizes ridiculously fast.
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