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Offlinebuggers
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question about liquid culture jar lid setup
    #5895041 - 07/24/06 05:32 PM (17 years, 6 months ago)

i'm setting up my lids as such. 2 lids with two one quarter inch holes drilled in each, the top lid has silicone applied to one of the holes for a self healing injection site. between the two lids iam putting a piece of tyvek (the tyvek is tyvek housewrap - this is the right stuff right?).

question: if i make quart jars of lc, how do you get the liquid into the syrings once the level of the liquid gets low in the jar? it seems like you can get some and then the last of the liquid is inaccessable.


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OfflineYamidude
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Re: question about liquid culture jar lid setup [Re: buggers]
    #5895048 - 07/24/06 05:33 PM (17 years, 6 months ago)

tilt and draw it out


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Offlinebuggers
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Re: question about liquid culture jar lid setup [Re: Yamidude]
    #5895057 - 07/24/06 05:36 PM (17 years, 6 months ago)

this wont get the tyvek wet and introduce contams will it?


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OfflineYamidude
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Re: question about liquid culture jar lid setup [Re: buggers]
    #5895076 - 07/24/06 05:44 PM (17 years, 6 months ago)

that's why you put the tyvek and the injection port on opposite sides


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OfflineYamidude
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Re: question about liquid culture jar lid setup [Re: Yamidude]
    #5895083 - 07/24/06 05:47 PM (17 years, 6 months ago)



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Offlinesplifner180
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Re: question about liquid culture jar lid setup [Re: Yamidude]
    #5895166 - 07/24/06 06:13 PM (17 years, 6 months ago)

IMO, the RTV+polyfill lids are the shiz.



Never had one contam, no vacuum when aspirating.

splif


--------------------
First Grow: Ecuador -> LC -> HPoo/Straw -> Monotub
Build a Do-It-Yourself Magnetic Stirrer in thirty minutes with no money.


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Offlinebuggers
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Re: question about liquid culture jar lid setup [Re: splifner180]
    #5895296 - 07/24/06 07:02 PM (17 years, 6 months ago)

yamidude you answered my question.

otherdude, what is rtv?


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Invisibleliamtheloser
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Re: question about liquid culture jar lid setup [Re: buggers]
    #5895304 - 07/24/06 07:04 PM (17 years, 6 months ago)

rtv = room temperature vulcanizing.


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Offlinebuggers
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Re: question about liquid culture jar lid setup [Re: liamtheloser]
    #5895353 - 07/24/06 07:21 PM (17 years, 6 months ago)

english please?


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Invisibleliamtheloser
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Re: question about liquid culture jar lid setup [Re: buggers]
    #5895504 - 07/24/06 07:58 PM (17 years, 6 months ago)

means it 'cures' or is hardened at room temperature... whereas other silicones may need heat or pressure to 'cure'.


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Offlinesplifner180
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Re: question about liquid culture jar lid setup [Re: liamtheloser]
    #5895969 - 07/24/06 09:38 PM (17 years, 6 months ago)

You can find it in any hardware store. It "sets" in about an hour and your PC won't put a dent in it.

splif


--------------------
First Grow: Ecuador -> LC -> HPoo/Straw -> Monotub
Build a Do-It-Yourself Magnetic Stirrer in thirty minutes with no money.


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OfflineYamidude
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Re: question about liquid culture jar lid setup [Re: splifner180]
    #5896183 - 07/24/06 10:26 PM (17 years, 6 months ago)

just ask for rtv sealant. I like to get mine at the auto parts stores. it's usually a little cheaper..


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Invisibleliamtheloser
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Re: question about liquid culture jar lid setup [Re: Yamidude]
    #5896191 - 07/24/06 10:28 PM (17 years, 6 months ago)

yeah, it's either at auto stores or in the automotive section of a big retail store.

It's called RTV Silicone Sealant. And it's for making temporary custom gaskets in car engines.


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OfflineYamidude
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Re: question about liquid culture jar lid setup [Re: liamtheloser]
    #5896206 - 07/24/06 10:31 PM (17 years, 6 months ago)

heres a link you can check out. It's not a shroomery sponsors link but none of the sponsors sell this item. Self healing rubber seals to use as injection ports. The work PERFECTLY.

http://www.gearboxinc.com/product_info.php?cPath=28&products_id=85

(if posting the link is a prob feel free to edit my post mods)


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