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OfflineEWalter69
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Registered: 04/16/06
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Last seen: 15 years, 9 months
Invitro Questions
    #5893992 - 07/24/06 10:44 AM (17 years, 6 months ago)

I have some BRF cakes that recently finished colonizing and I will be growing them invitro. Some questions:

1. Is it better to keep the jars right side up or upside down? I figure upside down is better because the cakes are conformed to the shape of the bottom of the jar and flipping them will allow more air around their sides, as well as better CO2 exchange.

2. Are the temperature requirements the same as with non-invitro cakes? A lot of condensation forms along the inside of the jars... should they ever be opened and wiped down or is it fine to just leave them?

3. My last two cakes were grown in poor-man's coke bottle terrariums, and something weird happened with them: they both developed a few pins that grew to about a 1/4 inch in length, then stopped. Nothing about their environment was changed. What could have caused this?


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Offlineroyer
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Registered: 05/15/06
Posts: 4,801
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Re: Invitro Questions [Re: EWalter69]
    #5894021 - 07/24/06 11:05 AM (17 years, 6 months ago)

the ones that grew 1/4 inch and stoped are called aborts


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OfflineRogerRabbitM
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Registered: 03/26/03
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Re: Invitro Questions [Re: royer]
    #5894076 - 07/24/06 11:27 AM (17 years, 6 months ago)

Lack of air. If you're growing invitro from pf cakes, dump the verm barrier out at full colonization to make room for the mushrooms and to give a bit of air. You might also drill a couple of extra holes in the lid for air exchange. Upside down is better than right side up, but laying them on their side is best.
RR


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