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Offlinetokyo_brites
Stranger
Registered: 07/24/06
Posts: 15
Last seen: 17 years, 3 months
Question about early mycelium growth on rye
    #5893813 - 07/24/06 08:23 AM (17 years, 6 months ago)

Hello. My friend is in this situation. He has inoculated 20 jars of pc d rye. He soaked the rye overnight, than simmered it for 45 minutes and pressure cooked for an hour. After inoculation the jars were shaken vigorously and left to sit in a dark place at about 78-80F. It has been 4 days, and he was wondering what sort of growth should be expected. On a few of the jars white fuzzy stuff can be spotted but it is in very small amounts. Only covering a single grain and in a few cases it has conjoined with 2 to 3 grains. Is this mycelium. What does mycelium look like in the very first stages of growth. He sacrificed one jar and opened it up to check moisture, etc. When looking through the glass of the jar it is difficult to spot any moisture, but doesn't look incredibly dry. The jar that was opened was inspected. He could split the rye grain and it was easy to tell that they were not crumbly and rather mushed around upon pressure. Also when the grains were opened they were white on the inside. Any input would be appreciated.


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InvisibleTippinthru
contented

Registered: 04/07/05
Posts: 1,131
Loc: "The Garden"...
Re: Question about early mycelium growth on rye [Re: tokyo_brites]
    #5893832 - 07/24/06 08:40 AM (17 years, 6 months ago)





Generally, experienced cultivators inoculate a spore solution, down the inside wall of a jar, in several area's.

Then, DO NOT SHAKE the jar.

That way, you can see colonization begin, quite plainly.

They then shake the jar at around 15 to 20% colonization.


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Perfection is attained by slow degrees; it requires the hand of time...
[


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Offlinethenewguy05
The Mushroom Man
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Registered: 02/11/05
Posts: 2,123
Loc: My Underground Layer
Last seen: 2 years, 5 months
Re: Question about early mycelium growth on rye [Re: Tippinthru]
    #5893849 - 07/24/06 09:01 AM (17 years, 6 months ago)

you shouldn't have very much growth at all yet. you have to take into account that you are using spores which are microscopic and they have to mate until fruitbody development. at four days you shouldn't have anything bigger than the size of a dime unless growing with LC and you said you aren't.

mycelium will look very spotty at first. unlike the above post i like to shake the jars to distribute the spore threw out the jar.

wait a week until the mycellium has grown out into a small mass or a few small masses(bigger than a quarter), and then shake the jar to evenly distribute the colonized grains. Only shake the jar this time and not again until it's time to use the spawn. shakeing anymore will slow your growth.


Edited by thenewguy05 (07/24/06 09:03 AM)


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InvisiblethatiAM
Stranger

Registered: 06/14/06
Posts: 1,250
Re: Question about early mycelium growth on rye [Re: thenewguy05]
    #5893927 - 07/24/06 10:05 AM (17 years, 6 months ago)

I always see people using grain as a spawn, never as something to fruit directly from itself. Is it a bad idea to just only grains (such as rye) to grow with?


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OfflineRogerRabbitM
Bans for Pleasure
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Registered: 03/26/03
Posts: 42,214
Loc: Seattle
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Re: Question about early mycelium growth on rye [Re: thatiAM]
    #5893969 - 07/24/06 10:35 AM (17 years, 6 months ago)

You can fruit directly from the grains by applying a casing layer, but performance is inhanced if you spawn to a bulk substrate such as manure or straw.

Quote from trippinthru. Generally, experienced cultivators inoculate a spore solution, down the inside wall of a jar, in several areas Then, DO NOT SHAKE the jar. That way, you can see colonization begin, quite plainly. They then shake the jar at around 15 to 20% colonization."

Good advice. The biggest reason to NOT shake right after inoculation is because the germinated spores each send out individual hyphae that must then 'mate' with compatible hyphae before dikaryotic(fertile) mycelium can form. If you shake right after inoculation with spores, these slow growing hyphae must travel long distances to find a 'partner'. If you leave them where they are injected, this travel time is much less and you get rapidly growing dikaryons much sooner. Shake when 30% or so colonized.
RR


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Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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Invisibleshroomerite
Apprentice


Registered: 06/09/06
Posts: 513
Re: Question about early mycelium growth on rye [Re: RogerRabbit]
    #15517373 - 12/14/11 07:25 PM (12 years, 1 month ago)

So what happens when you shake to early and the jars colonizes with the lighter thinner mono myc with spots of the thicker ropier dikaryons. I have some jars that are 100% but a percentage of the grains are covered in a light thin mycelium. Are these safe to use for g2g? Will the grains inoculate the bulk sub and mate or should I wait until the thicker dikaryrons overgrow the rest of the grains?


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  "For best results, learn to work with nature rather than against it. Mycelium has an amazing ability to cope with less than optimal conditions, and will often fruit when a grower does everything wrong. However, do everything right and watch your performance go through the roof." RR



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Invisibleshroomerite
Apprentice


Registered: 06/09/06
Posts: 513
Re: Question about early mycelium growth on rye [Re: shroomerite]
    #15519845 - 12/15/11 09:23 AM (12 years, 1 month ago)

anyone ever experience this?


--------------------
  "For best results, learn to work with nature rather than against it. Mycelium has an amazing ability to cope with less than optimal conditions, and will often fruit when a grower does everything wrong. However, do everything right and watch your performance go through the roof." RR



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OfflineLoveLightPeace
Male

Registered: 08/26/11
Posts: 286
Last seen: 4 years, 11 months
Re: Question about early mycelium growth on rye [Re: Tippinthru]
    #18557745 - 07/14/13 03:19 PM (10 years, 6 months ago)

Quote:

Tippinthru said:




Generally, experienced cultivators inoculate a spore solution, down the inside wall of a jar, in several area's.

Then, DO NOT SHAKE the jar.

That way, you can see colonization begin, quite plainly.

They then shake the jar at around 15 to 20% colonization.






When u say "experienced cultivators inoculate a spore solution, down the inside wall of a jar, in several area's", then howcome in ur pictures the jars have only one hole for inoculation?!!


--------------------
I am so small I can barely be seen.
How can this great love be inside me?

Look at your eyes. They are small,
but they see enormous things.

(Rumi)


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OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 10 hours
Trusted Cultivator
Re: Question about early mycelium growth on rye [Re: LoveLightPeace]
    #18558021 - 07/14/13 04:20 PM (10 years, 6 months ago)

Because you can turn the needle to point to any side you want and then squirt the solution.


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