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zeegos
Shroomagator


Registered: 04/03/06
Posts: 827
Loc: bat country
Last seen: 3 years, 24 days
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Acetone for sterilization?
#5893618 - 07/24/06 05:34 AM (17 years, 6 months ago) |
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Will pure acetone sterilize equipment and such like rubbing alcohol does? Its all i got at the mo and i need to make sporeprints/syringes. And i aint one who lives near shops
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royer
±±±±±±±±±±

Registered: 05/15/06
Posts: 4,801
Loc: anywhere but here
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Re: Acetone for sterilization? [Re: zeegos]
#5893632 - 07/24/06 05:43 AM (17 years, 6 months ago) |
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bleach water will work. Lysol, alcohol , i have used vodka and grain alcohol too
-------------------- ================================================= if you have any questions please feel free to pm me , thx :-)
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zeegos
Shroomagator


Registered: 04/03/06
Posts: 827
Loc: bat country
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Re: Acetone for sterilization? [Re: royer]
#5893636 - 07/24/06 05:46 AM (17 years, 6 months ago) |
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Wont bleach water leave harmful residues on foil for taking sporeprints? Alcohol will do me fine if that works. Then i can use acetone afterwards to evaporate off all liquids.
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thenewguy05
The Mushroom Man


Registered: 02/11/05
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Re: Acetone for sterilization? [Re: zeegos]
#5893703 - 07/24/06 06:58 AM (17 years, 6 months ago) |
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use brand new sheets of foil and don't use the edges and you should be fine.
alcohol is my choice for sterilization. i know bleach also works but i wouldn't use anything else besides the two.
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gorgebush
Stranger

Registered: 07/16/01
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Re: Acetone for sterilization? [Re: zeegos]
#5893840 - 07/24/06 08:53 AM (17 years, 6 months ago) |
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most rubbing alchol is only 70%. If you shop at drug store you can find 91% alchol(anti-microbial) this is what you want. you can also find anti-microbial wipes in little presealed wipes. I use 100% alchol in my sterlize lamp, that leaves no residue thus no wiping after heat.
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zeegos
Shroomagator


Registered: 04/03/06
Posts: 827
Loc: bat country
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Re: Acetone for sterilization? [Re: gorgebush]
#5893841 - 07/24/06 08:54 AM (17 years, 6 months ago) |
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Ok, thanks Mr Bush
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creamcorn
mad scientist


Registered: 03/13/06
Posts: 2,962
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Re: Acetone for sterilization? [Re: gorgebush]
#5893872 - 07/24/06 09:18 AM (17 years, 6 months ago) |
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Quote:
gorgebush said: most rubbing alchol is only 70%. If you shop at drug store you can find 91% alchol(anti-microbial) this is what you want. you can also find anti-microbial wipes in little presealed wipes. I use 100% alchol in my sterlize lamp, that leaves no residue thus no wiping after heat.
70% rubbing alcohol is more effective at sterilizing surfaces than 91%... it is better able to penetrate contam spores, whereas 91% dehydrates the spores rather than breaches them. it is also slower evaporating so it has a longer time frame to do its job.
not to split hairs but there's no such thing as 100% alcohol. even reagent grade "anhydrous" alcohol is around 99.8% and it drops rapidly when exposed to air... its expensive, dangerous, and isn't something you pick up at the hardware store, it would have to come from a chemical supply house or the like. you'd want to use denatured alcohol in an alcohol lamp ideally, if you're trying to avoid soot/residue.
and to the original poster, no, acetone is not an effective surface sterilizer. it will not kill contaminate spores.
for good measure, here's a reference - keep in mind the times they have there, and remember if you're wiping it on a surface it evaporates in seconds. they had trouble getting it to kill things even with 20 minutes worth of contact.
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zeegos
Shroomagator


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Posts: 827
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Re: Acetone for sterilization? [Re: creamcorn]
#5893881 - 07/24/06 09:23 AM (17 years, 6 months ago) |
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well these caps are gonna be ready soon and i wont be able to get rubbing alcohol today. Its either vodka, dilute bleach or some peroxide.
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Sinthetic
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Re: Acetone for sterilization? [Re: zeegos]
#5894178 - 07/24/06 12:15 PM (17 years, 6 months ago) |
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Bleach.
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fastfred
Old Hand



Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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Re: Acetone for sterilization? [Re: Sinthetic]
#5896009 - 07/24/06 09:46 PM (17 years, 6 months ago) |
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Nice reference on the acetone CC. Acetone won't stay around long enough to sterilize anything. It might gass you out from the fumes or cause a fire though.
One point though, you can get pretty much pure isopropyl, it's red "heet". Found in every gas station. Yellow heet is methanol.
Ethanol is what is problematic to get in anhydrous form. Denatured alcohol works fine though for most situations, it's ethanol with just enough methanol to make it poisonous usually. It's pretty much anhydrous.
-FF
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gorgebush
Stranger

Registered: 07/16/01
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Re: Acetone for sterilization? [Re: creamcorn]
#5896174 - 07/24/06 10:25 PM (17 years, 6 months ago) |
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when i say 100% alcohol , mean de-natured alcohol. Which has some methynol added to it. It can be purchased anywhere that sell paint products. It is used in alcohol lamps. Alcohol alone should never be used to steralize. What i was saying if one doesn't have a alcohol lamp. And after heating scaple,spoon,knife ect. If there is a carbon black residue he should wipe it clean with 91% rubbing alcohol. I was under the impression that 70% alcohol and 30% water would evaporate slower than 91% alcohol and 9% water. regardless it doesnt matter the heat should do the job. I ust to use a bic and soaked papertowel. And after a glovebox accident where i lost my eyelashes,soulpatch,minor burns i forked out the couple dollars to myco supply for steralize lamp. Although one could use a butane lighter which burns without residue, or one could even leave risdue on especially if working in a glovebox. Becouse they can go BOOOM
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