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Offlinedark_star5877
Stranger
Registered: 07/22/06
Posts: 5
Last seen: 17 years, 5 months
Newbie needs help, one strain colonized, 2 others did not
    #5887370 - 07/22/06 12:18 PM (17 years, 6 months ago)

I have searched, but cannot find an answer to my problem. I have tried the PF strain twice, the hawaiian strain once, and the John Allen strain once, all syringes. The only one that would take is the John Allen. I used the PF Tech, with brown rice flour cakes for all of them. And I tried the Bird Seed method for the PF strain as well. I used the same conditions for all, in an incubator at about 84 degrees. I'm at a loss for why 1 will colonize and the others will do nothing, if the conditions are the same. The only thing I can think is that maybe the water content might have varied. Will too much water keep spores from growing? The birdseed jars looked more dry than wet. I got the spores from vendors recomended from this site. I have cased the John Allen and will be fruiting in a couple of days. I am excited about that, mycology fascinates me, but I am disappointed about the other two strains. Also, if the spores don't grown in the jars, are they still alive, and can they be started by doing something to the jars? Patience and help very appreciated.


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Offlinestormhalter
full metalalchemist

Registered: 07/10/06
Posts: 175
Loc: Behind you.
Last seen: 16 years, 6 months
Re: Newbie needs help, one strain colonized, 2 others did not [Re: dark_star5877]
    #5887399 - 07/22/06 12:29 PM (17 years, 6 months ago)

Where did you purchase your spores from?


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Offlinedark_star5877
Stranger
Registered: 07/22/06
Posts: 5
Last seen: 17 years, 5 months
Re: Newbie needs help, one strain colonized, 2 others did not [Re: stormhalter]
    #5887508 - 07/22/06 01:07 PM (17 years, 6 months ago)

I bought PF, Hawaii, and John Allen from Spores 101, and bought 1 PF from the hawkseye. The PF from Hawks has been in jars for 8 days with no sign of life. The PF and Hawaiian from spores 101 were in jars in incubator for about a month before I threw them out. Also, I did let the jars cool for 24 hours before inoculating.


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Invisibleliamtheloser
Advanced Idiot
Male User Gallery
Registered: 06/07/06
Posts: 1,453
Re: Newbie needs help, one strain colonized, 2 others did not [Re: dark_star5877]
    #5887797 - 07/22/06 02:47 PM (17 years, 6 months ago)

sounds like a sneaky bacterial contam to me... either that or you havent been shaking your syringes like a madman before innocing.


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