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Offlinejben
Don't panic, it's organic
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Registered: 05/24/04
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my first try with B+
    #5886929 - 07/22/06 08:09 AM (17 years, 6 months ago)

Hey guys! i started 8 half pint B+ a few weeks ago, 5 days went by and only 2/8 showed any growth, the others just smelled real rotten. now I only have one jar at 3 weeks thats only maybe 50% colonized. I might end up throwing it out?I'm wondering what your method of sterilizing is? I'm almost positive I lost all my jars due to bacteria that was not killed in the pressure cooker. I realized the endospores made it thru the cooking. I also flame sterilized the needle so I don't think that the needle had anything to do with my failure. next time i do a run, im going to soak brown rice flour for 24 hours, then drain,rinse, then pressure cook the substrate for 90 min at 15psi.. does this sound like a good idea? maybe i'll have better luck with these results?  :mushroom2: :stoned:


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Don't panic, it's organic!


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Offlineentelligence
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Registered: 01/17/05
Posts: 140
Loc: The Great Outdoors
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Re: my first try with B+ [Re: jben]
    #5887117 - 07/22/06 10:16 AM (17 years, 6 months ago)

Sounds like a good idea. I don't think you would need to soak the BRF though. You're going to want to start narrowing down the point in which you are letting contams in. You can usually rule out inoculation by following a good glove box tek.


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First Casing - B+ on HPoo


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Offlinetonyperez420
Shaman Rasta

Registered: 11/03/04
Posts: 1,234
Last seen: 10 years, 11 months
Re: my first try with B+ [Re: jben]
    #5887161 - 07/22/06 10:39 AM (17 years, 6 months ago)

Quote:

jben said:
Hey guys! i started 8 half pint B+ a few weeks ago, 5 days went by and only 2/8 showed any growth, the others just smelled real rotten. now I only have one jar at 3 weeks thats only maybe 50% colonized. I might end up throwing it out?I'm wondering what your method of sterilizing is? I'm almost positive I lost all my jars due to bacteria that was not killed in the pressure cooker. I realized the endospores made it thru the cooking. I also flame sterilized the needle so I don't think that the needle had anything to do with my failure. next time i do a run, im going to soak brown rice flour for 24 hours, then drain,rinse, then pressure cook the substrate for 90 min at 15psi.. does this sound like a good idea? maybe i'll have better luck with these results?  :mushroom2: :stoned:




where did you get your spores?


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Offlinestormhalter
full metalalchemist

Registered: 07/10/06
Posts: 175
Loc: Behind you.
Last seen: 16 years, 6 months
Re: my first try with B+ [Re: tonyperez420]
    #5887178 - 07/22/06 10:49 AM (17 years, 6 months ago)

I just started my first cultivation six days ago, using P.Cubensis (Blue Meanies). I started off with four jars as this is my first attempt. I didn't want to get ahead of myself, and if I did fail, I didn't want to fail on a large scale and lose material.

So far so good. 4/4 jars are colonized about 60%. I used a pressure canner (AA 921 21 QT) like you and have been following the PF Tek/PF Tek For Simple Minds. The only thing I did different from you is buy organic brown rice and grind it in a Black & Decker Pulse Coffee Grinder. I find this is safer as you never know how long already ground up rice (BRF) has been sitting around, and how long it's been on the shelves.

Try grinding your own rice instead. (:


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InvisibleFooMan
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Re: my first try with B+ [Re: jben]
    #5887209 - 07/22/06 11:00 AM (17 years, 6 months ago)

There are no endospores in brown rice flour. Endospores are spores that are protected within the grain. Once the grain is ground, the endospores are exposed. I would up your PC time and make sure you use the correct ratio of 2:1:1- verm:BRF:H2O. Also, let your jars cool in the PC overnight. If 2 of your jars colonized, than I doubt it was the spores. It could have been the needle if you didn't sterilize it between each jar.

Are you doing the actual PF tek with verm, BRF and water? I only ask because you only mentioned BRF.


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Quick WBS Prep


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Invisiblecreamcorn
mad scientist
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Registered: 03/13/06
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Re: my first try with B+ [Re: FooMan]
    #5887324 - 07/22/06 12:01 PM (17 years, 6 months ago)

next time you do your jars, prepare an extra, sterilize it and incubate it and treat it exactly like the other jars, but do not inoculate it with any spores.

this helps narrow down where the problem comes form. i had problems with bacteria at a point, thought it was insufficient sterilization, made a few of these "control" jars and noticed when i'd get smelly jars the control jars always were fine. i traced it back in my case to a bacterial contaminated LC.... but at least by doing that it told me i was introducing the bacteria when i inoculated.


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Offlinejben
Don't panic, it's organic
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Registered: 05/24/04
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Re: my first try with B+ [Re: tonyperez420]
    #5888585 - 07/22/06 07:04 PM (17 years, 6 months ago)

the spores came from hawks eye which seem to be the most legit company around that I came across. Yes I did the Pf tek, although I did not sterilize before doing each jar... my next go I will up my pc time, flame sterilize between each jar and make an extra test jar or two with no spores in it. Oops and storm, i did grind my own brown rice. thanks for the help


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Don't panic, it's organic!


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Offlinegoots
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Registered: 07/18/06
Posts: 45
Last seen: 15 years, 10 days
Re: my first try with B+ [Re: creamcorn]
    #5888629 - 07/22/06 07:24 PM (17 years, 6 months ago)

Good call on the control jars, it never crossed my mind


Edited by goots (07/22/06 07:26 PM)


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Offlinejben
Don't panic, it's organic
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Registered: 05/24/04
Posts: 931
Last seen: 8 years, 4 months
Re: my first try with B+ [Re: goots]
    #5888645 - 07/22/06 07:32 PM (17 years, 6 months ago)

Yea, it seems like it could be a helpful idea. it would even be better to keep experimenting with control jars before i even innoc. again until I'm sure I have the contams handled.


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Don't panic, it's organic!


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OfflineSlainhobo
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Registered: 07/02/06
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Re: my first try with B+ [Re: jben]
    #5889346 - 07/22/06 11:53 PM (17 years, 6 months ago)

This was my first time at anything like this. I didn't have a pressure cooker when I did mine, just used a pot of boiling water. Only fit in 5, so I decided what the hell and microwaved the last one for 3 minutes on high. Everything else I kept super clean, gloves, the needle, shutting windows and turning off fans, and etc. No contams at all. Just think, medical clean. Just dunked and rolled last week, got some nice pins popping up :cool:.


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Offlineprincestoadstool
Pot Head
Female

Registered: 07/05/06
Posts: 40
Loc: bouncin' around the room
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Re: my first try with B+ [Re: Slainhobo]
    #5889467 - 07/23/06 12:44 AM (17 years, 6 months ago)

Personally, I haven't had any problems with contamination...yet. Having said that, I sometimes pre-sterilize my substrate ingredients before mixing them. I would bake my homemade BRF and vermiculite for 30 minutes at 320 before I mixed the substrate. Then fill the jars and boil them in a regular stock pot for an hour. No contamination. Now I just use the PC, because it's SO much more convenient..and still no contam.


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