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Invisiblezyxwvutsr
rstuvwxyz


Registered: 12/30/07
Posts: 141
Re: Increasing potency with 5-HTP (trytophan) [Re: RogerRabbit]
    #7895907 - 01/18/08 01:51 AM (16 years, 15 days ago)

Quote:

RogerRabbit said:
I'm still waiting for the talk to end and somebody do something. I've been watching this and other threads for years, but nobody has demonstrated that adding anything to the substrate will increase potency or that mushroom mycelium can convert one substance into another.

You can scroll down for thousands of posts and find this or similar threads repeated time and again. Will somebody please quit talking about it and just do it.
RR




I tried using mushrooms as mushroom substrate.
ground mush and verm in a 3:1 ratio yielded no result when inoculated via spore syringe.
Jar 2 knocked with LC showed 25% growth before stalling.
Spawn (wbs) failed to colonize substrate (cube mushrooms) at all.

Noticed water content difficult to adjust with mushrooms as substrate.
Mushroom substrate (spawn attempt 1) seemed to have fermented during incubation (84.3F). (spawn attempt 2 was incubated at room temp.)

General conclusion: Waste of Mushrooms. On the upside, one of one thousand future ponderers of this question will be educated upon reading this post and save their mushrooms.

Rodger Rabbit: I am new to the shroomery and suprised to find such experiments haven't been conducted. Hope this helps although very vague.


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Cube Growth Parameters


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OfflineRogerRabbitM
Bans for Pleasure
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Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Re: Increasing potency with 5-HTP (trytophan) [Re: zyxwvutsr]
    #7897015 - 01/18/08 11:09 AM (16 years, 14 days ago)

Thanks for reporting that. It doesn't make sense for mushrooms to be cannibalistic, but the question comes up a lot.
RR


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Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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OfflineLoveall
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Registered: 01/02/18
Posts: 8
Last seen: 2 months, 5 days
Re: Increasing potency with 5-HTP (trytophan) [Re: fastfred]
    #25429032 - 09/01/18 05:31 PM (5 years, 4 months ago)

Quote:

fastfred said:
I think that will do nothing.  Active mushrooms are good at 4-hydroxylating indoles and tryptamines, but it's going to give different products.

You'll just end up with 4-hydroxylated or phosphorylated IBA.  The carboxylic acid group is going to be reactive, so who knows what you'll end up with on that end.  It would be an interesting experiment, but I would be careful.  You never know what might turn out to be highly toxic.


-FF




^^ this.

I want to update an old post because I was thinking about adding 5-HTP to the substrate. I almost did the experiment but it turns out there could be a safety issue.

There is a chance that the mushroom could produce the neurotoxin  4,5-dihydroxytryptamine.

In general, this risk exists with any 5-OH substituted tryptamine.

I'm not saying the double hydroxy will form, but that there is a chance that it could. Mushrooms are very good at hydrolizing the 4-position.

I want to say: proceed with extreme caution. Best case you get bufotenine by adding 5-HTP, intermediate you could get nothing new, but worst case you get a neurotoxin.

Thank you and take care.


Edited by Loveall (09/01/18 05:33 PM)


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