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mushnoon
MushNoob

Registered: 05/13/06
Posts: 1,080
Last seen: 7 years, 11 months
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syringe help
#5884663 - 07/21/06 02:26 PM (17 years, 6 months ago) |
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Ok so here is my problem. the only syringes that I was able to obtain were 1CC 30gg syringes. The 1CC part just makes the task more tedious. However I have a feeling that the 30gg needle is having trouble sucking up spores. Is my spore solution not mixed well enough or do I need to acquire a different syringe and needle? When I suck up the solution is doesnt appear dark at all. Ill post pictures later tonight when I attempt the process again. I really need to just head over to home depot and get new ones. However my lack of a car and money is preventing me from doing that right now
-------------------- I love the feeling when it falls apart. Im slow to finish but im quick to start.
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Yamidude
Stranger

Registered: 06/15/06
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Re: syringe help [Re: mushnoon]
#5884814 - 07/21/06 03:16 PM (17 years, 6 months ago) |
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those are terrible. Do not use diabetic needles. You need alot larger gauge needle and larger capacity syring if you ask me. You can't even push oil based injectables through a 30G needle. www.gearboxinc.com is where i order all my syringes, needles, and self healing rubber seals. BTW i know spores are microscopic and will probably fit through a 30G needle but gimme a break already,
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splifner180
Student


Registered: 03/24/06
Posts: 1,288
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Re: syringe help [Re: mushnoon]
#5884819 - 07/21/06 03:17 PM (17 years, 6 months ago) |
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I'm not quite sure what those numbers mean. Are you saying that the volume of the syringe itself is 1cc and the needle is 30 gague?
splif
-------------------- First Grow: Ecuador -> LC -> HPoo/Straw -> Monotub Build a Do-It-Yourself Magnetic Stirrer in thirty minutes with no money.
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Yamidude
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Registered: 06/15/06
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yeah he has what seems to be very slim insulin pins. Most insulin pins aren't even that small. I believe the norm is 27Gauge
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mushnoon
MushNoob

Registered: 05/13/06
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Re: syringe help [Re: Yamidude]
#5884894 - 07/21/06 03:53 PM (17 years, 6 months ago) |
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yeah they are insulin pins. so should I even give them another try or is it just a waste of my time?
-------------------- I love the feeling when it falls apart. Im slow to finish but im quick to start.
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splifner180
Student


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Re: syringe help [Re: mushnoon]
#5884907 - 07/21/06 04:00 PM (17 years, 6 months ago) |
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Hm. Well, so long as you go through the hassle of flaming/alcohol swabbing the thing for every jar, go for it.
Sounds like a wicked PITA, though. And you said it was choking on spores?? Crimmeny.
splif
-------------------- First Grow: Ecuador -> LC -> HPoo/Straw -> Monotub Build a Do-It-Yourself Magnetic Stirrer in thirty minutes with no money.
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mushnoon
MushNoob

Registered: 05/13/06
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ok so ive decided that insulin syringes suck and Im not going to use them. Tell me if my new idea is kind of stupid or completely stupid. my local grocery store carries huge syringes with a very large gauge needle, often used when cooking a turkey to inject various juices? Think its worth a shot?
-------------------- I love the feeling when it falls apart. Im slow to finish but im quick to start.
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Roadkill
Retired Shroomery Mod


Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: syringe help [Re: mushnoon]
#5885079 - 07/21/06 05:17 PM (17 years, 6 months ago) |
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Quote:
mushnoon said:
ok so ive decided that insulin syringes suck and Im not going to use them. Tell me if my new idea is kind of stupid or completely stupid. my local grocery store carries huge syringes with a very large gauge needle, often used when cooking a turkey to inject various juices? Think its worth a shot?
yes the turky ones will work.
tc
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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mushnoon
MushNoob

Registered: 05/13/06
Posts: 1,080
Last seen: 7 years, 11 months
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Re: syringe help [Re: Roadkill]
#5885154 - 07/21/06 05:39 PM (17 years, 6 months ago) |
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awesome, thanks. another quick question. since the gauge of the needle is so large, will I need to replace my coffee filter after puncturing it ?
-------------------- I love the feeling when it falls apart. Im slow to finish but im quick to start.
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splifner180
Student


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Re: syringe help [Re: mushnoon]
#5885175 - 07/21/06 05:43 PM (17 years, 6 months ago) |
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The coffee filter is simply a dust cover. You shouldn't be puncturing it.
Can you tell us a bit more about your setup?
splif
-------------------- First Grow: Ecuador -> LC -> HPoo/Straw -> Monotub Build a Do-It-Yourself Magnetic Stirrer in thirty minutes with no money.
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mushnoon
MushNoob

Registered: 05/13/06
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hmmm I think after reading a few other threads ive discovered a mistake. I was attempting to use coffee filters as my gas exchange filter. (Meijer didnt have any tyvek filters) Ive already PCed my jars with WBS about 1/2 - 2/3 full. Any way to save these?
-------------------- I love the feeling when it falls apart. Im slow to finish but im quick to start.
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Yamidude
Stranger

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Re: syringe help [Re: mushnoon]
#5885195 - 07/21/06 05:50 PM (17 years, 6 months ago) |
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uhh hmm.. You need something better for exchange i would think. Got to wally world and get you a bag of pollyfill or go to the post office tomorrow and get some tyvek envelopes and then re-doo the jars IMO..
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mushnoon
MushNoob

Registered: 05/13/06
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Re: syringe help [Re: Yamidude]
#5885223 - 07/21/06 05:59 PM (17 years, 6 months ago) |
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Quote:
Yamidude said: uhh hmm.. You need something better for exchange i would think. Got to wally world and get you a bag of pollyfill or go to the post office tomorrow and get some tyvek envelopes and then re-doo the jars IMO..
is that pretty much my only option? Im poor and just gathered all of my change for the syringe. The bike ride to wally world would be pretty intense, as would the bike ride to the post office. Are the tyvek envelopes sufficient? and how much do those cost and how much does polyfill cost? Im on an extremely strict budget, as in I only have $3.50 and most of thats for the syringe
-------------------- I love the feeling when it falls apart. Im slow to finish but im quick to start.
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splifner180
Student


Registered: 03/24/06
Posts: 1,288
Loc: USA, East Coast
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Re: syringe help [Re: Yamidude]
#5885228 - 07/21/06 06:00 PM (17 years, 6 months ago) |
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You MIGHT be able to save them if you keep moisture off them at all costs but frankly ...I'd do them over.
And you can get tyvek at any US Post Office. They're the free mailers they give away. Heck, you can even order them from the USPO website and they'll send them to you for free.
splif
-------------------- First Grow: Ecuador -> LC -> HPoo/Straw -> Monotub Build a Do-It-Yourself Magnetic Stirrer in thirty minutes with no money.
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mushnoon
MushNoob

Registered: 05/13/06
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Last seen: 7 years, 11 months
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does the tyvek go over the lid and under the ring? or under the lid and ring? I ask this because my coffee filter is over the lid and under the ring. Would removing the coffee filter and slapping on some tyvek work or is that just asking for contams?
-------------------- I love the feeling when it falls apart. Im slow to finish but im quick to start.
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Roadkill
Retired Shroomery Mod


Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: syringe help [Re: mushnoon]
#5885306 - 07/21/06 06:27 PM (17 years, 6 months ago) |
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mushnoon,
I just made up my method of making spore sryinges from prints in my journal...
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/5885285/an/0/page/0
you might find this method of making up syringes helpful.
it's very newbie friendly!~

tc
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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mushnoon
MushNoob

Registered: 05/13/06
Posts: 1,080
Last seen: 7 years, 11 months
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Re: syringe help [Re: Roadkill]
#5885363 - 07/21/06 06:43 PM (17 years, 6 months ago) |
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thats very helpful indeed and ill more than likely use it tomorrow. still not sure about the placement of the tyvek. can anyone help me?
-------------------- I love the feeling when it falls apart. Im slow to finish but im quick to start.
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splifner180
Student


Registered: 03/24/06
Posts: 1,288
Loc: USA, East Coast
Last seen: 1 year, 3 months
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Re: syringe help [Re: Roadkill]
#5885376 - 07/21/06 06:48 PM (17 years, 6 months ago) |
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mushnoon writes: "does the tyvek go over the lid and under the ring? or under the lid and ring? I ask this because my coffee filter is over the lid and under the ring. Would removing the coffee filter and slapping on some tyvek work or is that just asking for contams? "
I do it in this order; jar, lid (with 1/4" hole drilled in center), tyvek, ring.
Yes, you could remove the ring and lift off the filter, replacing it with tyvek BUT you stand a somewhat decent chance of introducing contams. Unless, of course, the hole in your lid has it's own filter (like polyfill).
Your odds would be FAR better if you had a flowhood or a glovebox.
splif
-------------------- First Grow: Ecuador -> LC -> HPoo/Straw -> Monotub Build a Do-It-Yourself Magnetic Stirrer in thirty minutes with no money.
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mushnoon
MushNoob

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Re: syringe help [Re: mushnoon]
#5885701 - 07/21/06 08:40 PM (17 years, 6 months ago) |
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well, im going to risk it and try to just slap the tyvek on and see what happens. Which actually brings me back to one of my past questions, do I replace the tyvek after I puncture it with the syringe?
-------------------- I love the feeling when it falls apart. Im slow to finish but im quick to start.
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splifner180
Student


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Re: syringe help [Re: mushnoon]
#5885715 - 07/21/06 08:49 PM (17 years, 6 months ago) |
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If you really want to do this, clean the hell out of the area you'll be working in. Hose it down with alcohol, Lysol, Oust, whatever. Wipe down all surfaces, yadda yadda yadda. Wash your hands, too.
Pre-cut your tyvek squares. Set up the jars. Then quickly do these steps.
1) Remove the ring. 2) Remove the coffee filter. 3) Replace with tyvek. 4) Replace and re-thread ring.
I'd re-wash my hands after every 3 jars. Once all the jars are done, go ahead and inoculate the jars.
But let me stress I think this is a waste of your spores. I'd STRONGLY recommend you make an extra LC on the side. It's cheap to make, only takes about 15 minutes of PCing and when the jars fail -- which they're likely to do -- you still have plenty of mycelium to knock up new jars.
splif
-------------------- First Grow: Ecuador -> LC -> HPoo/Straw -> Monotub Build a Do-It-Yourself Magnetic Stirrer in thirty minutes with no money.
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mushnoon
MushNoob

Registered: 05/13/06
Posts: 1,080
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Re: syringe help [Re: mushnoon]
#5885790 - 07/21/06 09:09 PM (17 years, 6 months ago) |
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^^^^ will do. I hate to be a pest and ask the same thing again but im confused as to whether the tyvek must be replaced or patched after it is punctured by the syringe. Maybe im just an idiot but im having a hard time understanding that.
-------------------- I love the feeling when it falls apart. Im slow to finish but im quick to start.
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liamtheloser
Advanced Idiot

Registered: 06/07/06
Posts: 1,453
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Re: syringe help [Re: mushnoon]
#5886107 - 07/21/06 11:04 PM (17 years, 6 months ago) |
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Dude, don't do all that shit..... Just go get some fucking micropore tape (in the first aid area... nexcare makes it in a scotch tape dipsenser). Just tape the holes and you don't have to remake the jars... I don't know why every is telling you to use tyvek and polyfil... micropore has worked almost as well as polyfil for me, and there's no reason you should have to re-do your jars just because you used a coffee filter instead..
And no, don't replace the tyvek after it's punctured by the syringe, it's a thick weave polyester material (like polyfil woven into a sheet of paper).
You'll be fine by just putting a piece of micropore tape over each hole for your first run though... Just make sure you remove the coffee filter and put the tape on in a room with NO DRAFTS no WINDOWS no MOVING AIR. I use a bathroom with no windows with the door closed.. I spray lysol on all surfaces then use oust on the air, let it all settle for 5 minutes while I get things ready, then inject.
I hope you love me.
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fastfred
Old Hand



Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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Just skip the whole filter idea. It's not needed.
Poke or drill a hole in the lids and cover with tinfoil. That's all you need to do.
-FF
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killalaz
Stranger
Registered: 07/22/06
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Re: syringe help [Re: mushnoon]
#5886443 - 07/22/06 01:49 AM (17 years, 6 months ago) |
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I wouldn't use insulin syringes for anything other than insulin. They clog very easily and are not meant to be used more than once so they fall apart. The plunger sometimes gets stuck and the plastic
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liamtheloser
Advanced Idiot

Registered: 06/07/06
Posts: 1,453
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Re: syringe help [Re: killalaz]
#5886445 - 07/22/06 01:51 AM (17 years, 6 months ago) |
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lots of insulin syringes also have a syringe filter on them too... not sure if that would filter out spores, but i'd rather use a nice 16-14 gauge 30cc syringe any day.
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splifner180
Student


Registered: 03/24/06
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mushnoon writes: "will do. I hate to be a pest and ask the same thing again but im confused as to whether the tyvek must be replaced or patched after it is punctured by the syringe. Maybe im just an idiot but im having a hard time understanding that. "
Yes.
Where you puncture the tyvek you should cover with micropore tape. Me, I like to pre-cut strips of micropore and let it sit in a petri dish filled with rubbing alcohol. After I inoculate three jars I re-apply an alcohol-soaked cotton ball around the needle itself and lay it someplace clean. I then pull each micropore strip out of the alcohol and place over the hole. The alcohol will evaporate, leaving the tape stuck to the tyvek.
liamtheloser writes: "Dude, don't do all that shit..... Just go get some fucking micropore tape (in the first aid area... nexcare makes it in a scotch tape dipsenser). Just tape the holes and you don't have to remake the jars... I don't know why every is telling you to use tyvek and polyfil... "
My understanding was that mushnoon accidentally used coffee filters (not tyvek or micropore) on his jars. Above the lid but below the ring. What he's attempting to do is swap out the coffee filters with tyvek.
Unless I seriously misread something...
splif
-------------------- First Grow: Ecuador -> LC -> HPoo/Straw -> Monotub Build a Do-It-Yourself Magnetic Stirrer in thirty minutes with no money.
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mushnoon
MushNoob

Registered: 05/13/06
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<I hope you love me. > I do, and even moreso I love your inspirational letters I think ill go the micropore route, sounds easiest. I did try using the coffee filters at first, I should have read the TEKs a litte closer. Ill learn from my mistake and give it another run.
-------------------- I love the feeling when it falls apart. Im slow to finish but im quick to start.
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liamtheloser
Advanced Idiot

Registered: 06/07/06
Posts: 1,453
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Re: syringe help [Re: mushnoon]
#5892719 - 07/23/06 10:57 PM (17 years, 6 months ago) |
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amen man... i've never had a problem using micropore tape... on some of my grain jars i've used one hole with polyfil, i didn't see any advantage... so micropore is my filter of choice.
and i'm glad you love me, because i love you and everyone else here so much
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