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mushnoon
MushNoob

Registered: 05/13/06
Posts: 1,080
Last seen: 7 years, 11 months
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syringe help
#5884663 - 07/21/06 02:26 PM (17 years, 6 months ago) |
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Ok so here is my problem. the only syringes that I was able to obtain were 1CC 30gg syringes. The 1CC part just makes the task more tedious. However I have a feeling that the 30gg needle is having trouble sucking up spores. Is my spore solution not mixed well enough or do I need to acquire a different syringe and needle? When I suck up the solution is doesnt appear dark at all. Ill post pictures later tonight when I attempt the process again. I really need to just head over to home depot and get new ones. However my lack of a car and money is preventing me from doing that right now
-------------------- I love the feeling when it falls apart. Im slow to finish but im quick to start.
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Yamidude
Stranger

Registered: 06/15/06
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Re: syringe help [Re: mushnoon]
#5884814 - 07/21/06 03:16 PM (17 years, 6 months ago) |
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those are terrible. Do not use diabetic needles. You need alot larger gauge needle and larger capacity syring if you ask me. You can't even push oil based injectables through a 30G needle. www.gearboxinc.com is where i order all my syringes, needles, and self healing rubber seals. BTW i know spores are microscopic and will probably fit through a 30G needle but gimme a break already,
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splifner180
Student


Registered: 03/24/06
Posts: 1,288
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Re: syringe help [Re: mushnoon]
#5884819 - 07/21/06 03:17 PM (17 years, 6 months ago) |
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I'm not quite sure what those numbers mean. Are you saying that the volume of the syringe itself is 1cc and the needle is 30 gague?
splif
-------------------- First Grow: Ecuador -> LC -> HPoo/Straw -> Monotub Build a Do-It-Yourself Magnetic Stirrer in thirty minutes with no money.
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Yamidude
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Registered: 06/15/06
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yeah he has what seems to be very slim insulin pins. Most insulin pins aren't even that small. I believe the norm is 27Gauge
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mushnoon
MushNoob

Registered: 05/13/06
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Last seen: 7 years, 11 months
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Re: syringe help [Re: Yamidude]
#5884894 - 07/21/06 03:53 PM (17 years, 6 months ago) |
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yeah they are insulin pins. so should I even give them another try or is it just a waste of my time?
-------------------- I love the feeling when it falls apart. Im slow to finish but im quick to start.
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splifner180
Student


Registered: 03/24/06
Posts: 1,288
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Re: syringe help [Re: mushnoon]
#5884907 - 07/21/06 04:00 PM (17 years, 6 months ago) |
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Hm. Well, so long as you go through the hassle of flaming/alcohol swabbing the thing for every jar, go for it.
Sounds like a wicked PITA, though. And you said it was choking on spores?? Crimmeny.
splif
-------------------- First Grow: Ecuador -> LC -> HPoo/Straw -> Monotub Build a Do-It-Yourself Magnetic Stirrer in thirty minutes with no money.
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mushnoon
MushNoob

Registered: 05/13/06
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Last seen: 7 years, 11 months
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ok so ive decided that insulin syringes suck and Im not going to use them. Tell me if my new idea is kind of stupid or completely stupid. my local grocery store carries huge syringes with a very large gauge needle, often used when cooking a turkey to inject various juices? Think its worth a shot?
-------------------- I love the feeling when it falls apart. Im slow to finish but im quick to start.
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Roadkill
Retired Shroomery Mod


Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: syringe help [Re: mushnoon]
#5885079 - 07/21/06 05:17 PM (17 years, 6 months ago) |
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Quote:
mushnoon said:
ok so ive decided that insulin syringes suck and Im not going to use them. Tell me if my new idea is kind of stupid or completely stupid. my local grocery store carries huge syringes with a very large gauge needle, often used when cooking a turkey to inject various juices? Think its worth a shot?
yes the turky ones will work.
tc
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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mushnoon
MushNoob

Registered: 05/13/06
Posts: 1,080
Last seen: 7 years, 11 months
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Re: syringe help [Re: Roadkill]
#5885154 - 07/21/06 05:39 PM (17 years, 6 months ago) |
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awesome, thanks. another quick question. since the gauge of the needle is so large, will I need to replace my coffee filter after puncturing it ?
-------------------- I love the feeling when it falls apart. Im slow to finish but im quick to start.
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splifner180
Student


Registered: 03/24/06
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Re: syringe help [Re: mushnoon]
#5885175 - 07/21/06 05:43 PM (17 years, 6 months ago) |
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The coffee filter is simply a dust cover. You shouldn't be puncturing it.
Can you tell us a bit more about your setup?
splif
-------------------- First Grow: Ecuador -> LC -> HPoo/Straw -> Monotub Build a Do-It-Yourself Magnetic Stirrer in thirty minutes with no money.
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mushnoon
MushNoob

Registered: 05/13/06
Posts: 1,080
Last seen: 7 years, 11 months
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hmmm I think after reading a few other threads ive discovered a mistake. I was attempting to use coffee filters as my gas exchange filter. (Meijer didnt have any tyvek filters) Ive already PCed my jars with WBS about 1/2 - 2/3 full. Any way to save these?
-------------------- I love the feeling when it falls apart. Im slow to finish but im quick to start.
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Yamidude
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Re: syringe help [Re: mushnoon]
#5885195 - 07/21/06 05:50 PM (17 years, 6 months ago) |
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uhh hmm.. You need something better for exchange i would think. Got to wally world and get you a bag of pollyfill or go to the post office tomorrow and get some tyvek envelopes and then re-doo the jars IMO..
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mushnoon
MushNoob

Registered: 05/13/06
Posts: 1,080
Last seen: 7 years, 11 months
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Re: syringe help [Re: Yamidude]
#5885223 - 07/21/06 05:59 PM (17 years, 6 months ago) |
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Quote:
Yamidude said: uhh hmm.. You need something better for exchange i would think. Got to wally world and get you a bag of pollyfill or go to the post office tomorrow and get some tyvek envelopes and then re-doo the jars IMO..
is that pretty much my only option? Im poor and just gathered all of my change for the syringe. The bike ride to wally world would be pretty intense, as would the bike ride to the post office. Are the tyvek envelopes sufficient? and how much do those cost and how much does polyfill cost? Im on an extremely strict budget, as in I only have $3.50 and most of thats for the syringe
-------------------- I love the feeling when it falls apart. Im slow to finish but im quick to start.
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splifner180
Student


Registered: 03/24/06
Posts: 1,288
Loc: USA, East Coast
Last seen: 1 year, 3 months
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Re: syringe help [Re: Yamidude]
#5885228 - 07/21/06 06:00 PM (17 years, 6 months ago) |
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You MIGHT be able to save them if you keep moisture off them at all costs but frankly ...I'd do them over.
And you can get tyvek at any US Post Office. They're the free mailers they give away. Heck, you can even order them from the USPO website and they'll send them to you for free.
splif
-------------------- First Grow: Ecuador -> LC -> HPoo/Straw -> Monotub Build a Do-It-Yourself Magnetic Stirrer in thirty minutes with no money.
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mushnoon
MushNoob

Registered: 05/13/06
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Last seen: 7 years, 11 months
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does the tyvek go over the lid and under the ring? or under the lid and ring? I ask this because my coffee filter is over the lid and under the ring. Would removing the coffee filter and slapping on some tyvek work or is that just asking for contams?
-------------------- I love the feeling when it falls apart. Im slow to finish but im quick to start.
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Roadkill
Retired Shroomery Mod


Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: syringe help [Re: mushnoon]
#5885306 - 07/21/06 06:27 PM (17 years, 6 months ago) |
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mushnoon,
I just made up my method of making spore sryinges from prints in my journal...
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/5885285/an/0/page/0
you might find this method of making up syringes helpful.
it's very newbie friendly!~

tc
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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mushnoon
MushNoob

Registered: 05/13/06
Posts: 1,080
Last seen: 7 years, 11 months
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Re: syringe help [Re: Roadkill]
#5885363 - 07/21/06 06:43 PM (17 years, 6 months ago) |
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thats very helpful indeed and ill more than likely use it tomorrow. still not sure about the placement of the tyvek. can anyone help me?
-------------------- I love the feeling when it falls apart. Im slow to finish but im quick to start.
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splifner180
Student


Registered: 03/24/06
Posts: 1,288
Loc: USA, East Coast
Last seen: 1 year, 3 months
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Re: syringe help [Re: Roadkill]
#5885376 - 07/21/06 06:48 PM (17 years, 6 months ago) |
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mushnoon writes: "does the tyvek go over the lid and under the ring? or under the lid and ring? I ask this because my coffee filter is over the lid and under the ring. Would removing the coffee filter and slapping on some tyvek work or is that just asking for contams? "
I do it in this order; jar, lid (with 1/4" hole drilled in center), tyvek, ring.
Yes, you could remove the ring and lift off the filter, replacing it with tyvek BUT you stand a somewhat decent chance of introducing contams. Unless, of course, the hole in your lid has it's own filter (like polyfill).
Your odds would be FAR better if you had a flowhood or a glovebox.
splif
-------------------- First Grow: Ecuador -> LC -> HPoo/Straw -> Monotub Build a Do-It-Yourself Magnetic Stirrer in thirty minutes with no money.
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mushnoon
MushNoob

Registered: 05/13/06
Posts: 1,080
Last seen: 7 years, 11 months
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Re: syringe help [Re: mushnoon]
#5885701 - 07/21/06 08:40 PM (17 years, 6 months ago) |
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well, im going to risk it and try to just slap the tyvek on and see what happens. Which actually brings me back to one of my past questions, do I replace the tyvek after I puncture it with the syringe?
-------------------- I love the feeling when it falls apart. Im slow to finish but im quick to start.
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splifner180
Student


Registered: 03/24/06
Posts: 1,288
Loc: USA, East Coast
Last seen: 1 year, 3 months
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Re: syringe help [Re: mushnoon]
#5885715 - 07/21/06 08:49 PM (17 years, 6 months ago) |
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If you really want to do this, clean the hell out of the area you'll be working in. Hose it down with alcohol, Lysol, Oust, whatever. Wipe down all surfaces, yadda yadda yadda. Wash your hands, too.
Pre-cut your tyvek squares. Set up the jars. Then quickly do these steps.
1) Remove the ring. 2) Remove the coffee filter. 3) Replace with tyvek. 4) Replace and re-thread ring.
I'd re-wash my hands after every 3 jars. Once all the jars are done, go ahead and inoculate the jars.
But let me stress I think this is a waste of your spores. I'd STRONGLY recommend you make an extra LC on the side. It's cheap to make, only takes about 15 minutes of PCing and when the jars fail -- which they're likely to do -- you still have plenty of mycelium to knock up new jars.
splif
-------------------- First Grow: Ecuador -> LC -> HPoo/Straw -> Monotub Build a Do-It-Yourself Magnetic Stirrer in thirty minutes with no money.
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