Sample from inner stem for cloning, too old?!?!?!? - Advanced Mycology

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Visigoth
enthusiast

Registered: 11/28/01
Posts: 481
Last seen: 11 years, 10 months

Sample from inner stem for cloning, too old?!?!?!?

#587558 - 03/23/02 06:39 PM (22 years, 8 months ago)

is there a point where a mushroom gets too old to take a tissue sample from and used w. agar for cloning?? Thanks!!!

Vis

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"15 minutes in freezing conditions?!?! You'd become a popsicle before you made that!!!"

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weatstraw
Stranger

Registered: 03/24/02
Posts: 15
Loc: Oregon
Last seen: 20 years, 7 months

Re: Sample from inner stem for cloning, too old?!?!?!?

[Re: Visigoth]
#587854 - 03/24/02 04:15 AM (22 years, 8 months ago)

Personally I've found that cloning from a third flush or sometimes even second flush specimen causes slow growth on a petri and even slower growth once I transfer the agar wedges to rye jars. I've started to only use first flush mushrooms if I'm going to clone. I suppose my advice needs to be taken with a grain of salt though, I've always cloned using the h2o2 petri tek, which has been known to slow groth in and of itself.