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OfflineVisigoth
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Registered: 11/28/01
Posts: 481
Last seen: 4 years, 7 months
Sample from inner stem for cloning, too old?!?!?!?
    #587558 - 03/23/02 08:39 PM (15 years, 6 months ago)

is there a point where a mushroom gets too old to take a tissue sample from and used w. agar for cloning?? Thanks!!!

Vis


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Offlineweatstraw
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Registered: 03/24/02
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Loc: Oregon
Last seen: 13 years, 5 months
Re: Sample from inner stem for cloning, too old?!?!?!? [Re: Visigoth]
    #587854 - 03/24/02 06:15 AM (15 years, 6 months ago)

Personally I've found that cloning from a third flush or sometimes even second flush specimen causes slow growth on a petri and even slower growth once I transfer the agar wedges to rye jars. I've started to only use first flush mushrooms if I'm going to clone. I suppose my advice needs to be taken with a grain of salt though, I've always cloned using the h2o2 petri tek, which has been known to slow groth in and of itself.


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Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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