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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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Blutjager
Inhuman


Registered: 06/11/06
Posts: 9,220
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Re: LC Cloning - Newbie friendly pictorial tek! [Re: CaptTrip]
#7203423 - 07/20/07 05:31 PM (16 years, 6 months ago) |
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As long as the bottle is shaped like a bear it will work
No for real,that all I ever use
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Peace
Life doesn't end @ your flesh...


Registered: 05/22/06
Posts: 156
Loc: Theraputic Mountain
Last seen: 12 years, 10 months
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Re: LC Cloning - Newbie friendly pictorial tek! [Re: creamcorn]
#7205369 - 07/21/07 03:17 AM (16 years, 6 months ago) |
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haha k thanks guys. I'll try doing a Large jar (Quart) with just the normal karo light, and inject it with 3-4ml of Puerto Ricans. Then in another Quart jar do the same but with Ecuador. Then with two half pint jars i will do a 50/50 mix (honey/karo) and the other with straight Honey and inject both with the same strain of either PR, Equador, Golden Teacher or B+... what ya think i should pick? First time growing successfully (got a P.Cooker finally) so not sure which would be best to test for the smaller jars.
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FellowGrower
Fungal Freedom Fighter



Registered: 05/25/07
Posts: 1,804
Last seen: 2 months, 10 days
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Re: LC Cloning - Newbie friendly pictorial tek! [Re: Peace]
#7205525 - 07/21/07 05:59 AM (16 years, 6 months ago) |
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I use Shards from the glass pile I have from bustin up jars, b/c as we have already said... ANY jar will work... http://www.shroomery.org/forums/showflat.php/Cat/0/Number/7136379/an/0/page/0
So, in this sense, you should have a bunch of sterile shattered glass anyway! right? lol
heres what mine looks like.
it started turnin red? you see it? in the top left. just below the surface, theres a web of myc floatin with red shit on it. and around the rim, just above the water level, little red specs stuck to the side? whats that mean?
If its bad news keep it to yourself k? lol Im kidden whats the deal?
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Mycodood



Registered: 05/22/07
Posts: 277
Loc: USA
Last seen: 2 years, 11 months
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Re: LC Cloning - Newbie friendly pictorial tek! [Re: FellowGrower]
#7214087 - 07/23/07 02:22 PM (16 years, 6 months ago) |
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Just a suggestion but it may be better to take the biopsy from the top of the cap straight through the middle of the stem. That way you only have to wipe the top and the tissue where the cap meets the stem is usually meaty.
Just a thought.
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xMoorhsuMx
Stranger



Registered: 08/25/07
Posts: 68
Last seen: 13 years, 1 month
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Re: LC Cloning - Newbie friendly pictorial tek! [Re: creamcorn]
#7345968 - 08/28/07 07:34 PM (16 years, 5 months ago) |
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Amazing Good job man
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ThePyschonaut52
Stranger


Registered: 04/24/07
Posts: 982
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Re: LC Cloning - Newbie friendly pictorial tek! [Re: xMoorhsuMx]
#7403863 - 09/13/07 06:07 AM (16 years, 4 months ago) |
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bump
-------------------- "In god we trust..." -I guess we're screwed.
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FellowGrower
Fungal Freedom Fighter



Registered: 05/25/07
Posts: 1,804
Last seen: 2 months, 10 days
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Re: LC Cloning - Newbie friendly pictorial tek! [Re: ThePyschonaut52]
#7404756 - 09/13/07 02:58 PM (16 years, 4 months ago) |
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how do You tell when a PC without a gauge is at 15psi?? thatnks.....
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veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 24 days
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Re: LC Cloning - Newbie friendly pictorial tek! [Re: liamtheloser]
#7447847 - 09/24/07 02:06 PM (16 years, 4 months ago) |
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This is the best tek i have read on cloning/LC. Im just waiting for pinning on my first cake (please pin and fruit!!!) and after reading this i will be cloning of my first cake.
I already have done 1 lc culture from spores (have no idea how it worked due to using a shot glas covered with tinfoil, and having to take the foil of and break up the myc with the needle without a glove box) i have a 1/2 pint pf tek already 100% colinesed in about 7 days just waiting to make sure it has a hold in the middle (though im sure it has as i inoced in the middle) then its birth. I just threw the rest out as it would have probably contamed.
This tek is so clear, that the only question that should every be asked is - could u simply inject that segment into a shroom and inoculate say a pf tek jar, or even a grain jar. I think it would work, it seems pretyy stupid though as u could only do it a couple of times then the generations would start to degrade and die. BUt if u got a growth from spores that had many good fruits, u could start a quite a few master cultures from various different fruits with qualities u may want to keep on going, then use another peice to start of more jars while u r waiting on the lc's growing.
5 shrooms for u!!!!
This should definatly be put into the teks and made the tek of choice. I have read probably all of them (as far as i can see from the cultivation section) and this is definatly the best.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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juende
feministpresence


Registered: 02/20/04
Posts: 729
Last seen: 8 years, 11 months
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Re: LC Cloning - Newbie friendly pictorial tek! [Re: veda_sticks]
#7447995 - 09/24/07 02:57 PM (16 years, 4 months ago) |
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wow how did i miss this. this is one amazing write up good job cubie!
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MonstroniuM
The Original Juggernaut



Registered: 09/02/07
Posts: 207
Loc: Florida, USA
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Re: LC Cloning - Newbie friendly pictorial tek! [Re: juende]
#7514487 - 10/14/07 01:43 AM (16 years, 3 months ago) |
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Totally didn't think that the clone jars needed gas exchange. I used the left over spores of my syringes after inoculation. There is def. mycelium in the jars, its just not cloudy/milky. There are little pieces, on the bottom, as well as floating. I take it is because I have had the jar lids screwed all the way down (I dont have a fancy gas exchange system like you, just one hole in the lid for the syringes with tape over it). I have just now after re-reading your post loosened the lid, but is it too late? I started the clones on, I want to say 9/20-23(?).
Thanks in advance!
-Monstro
-------------------- The text and pictures posted by this user are entirely fictional. All posts are for entertainment purposes only and do not necessarily reflect the views or beliefs of this user.
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fastfred
Old Hand



Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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Re: LC Cloning - Newbie friendly pictorial tek! [Re: MonstroniuM]
#7517455 - 10/14/07 08:56 PM (16 years, 3 months ago) |
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> Totally didn't think that the clone jars needed gas exchange.
They don't. I don't know where you're getting that from, but LCs need no gas exchange except for the pressure equalization required to prevent the jar from sucking in an entire syringe when you inoculate.
> There is def. mycelium in the jars, its just not cloudy/milky.
You don't want your LC to be cloudy or milky. That is a sign of bacterial or yeast contamination. All you want is to be able to see myc and have the rest of the liquid be clear.
If you do open up your LCs and any contaminant filled air gets inside you will indeed end up with a cloudy, milky, contaminated, useless mess.
-FF
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MonstroniuM
The Original Juggernaut



Registered: 09/02/07
Posts: 207
Loc: Florida, USA
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Re: LC Cloning - Newbie friendly pictorial tek! [Re: fastfred]
#7518019 - 10/14/07 11:18 PM (16 years, 3 months ago) |
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Well thank you for clearing it up for me, I can sleep easier now. I didn't open them up I just unscrewed the cap a wee bit so it wasn't incredibly tight, but have since fixed that. Thank you FF.
-------------------- The text and pictures posted by this user are entirely fictional. All posts are for entertainment purposes only and do not necessarily reflect the views or beliefs of this user.
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HeadFood
Shroomer



Registered: 12/30/07
Posts: 390
Last seen: 11 years, 1 month
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Re: LC Cloning - Newbie friendly pictorial tek! [Re: creamcorn]
#7841362 - 01/06/08 08:58 PM (16 years, 25 days ago) |
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Been doing my research for the past 2/3 weeks. I finally understand what an LC really is. It seems so much easier and more effective than a grain to grain transfer. Quick question, do you use the same amount of LC as you do with a spore Syringe? Thanks for such a HUGE help!
-------------------- Phi Alpha
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fastfred
Old Hand



Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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Re: LC Cloning - Newbie friendly pictorial tek! [Re: HeadFood]
#7842252 - 01/07/08 03:42 AM (16 years, 25 days ago) |
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Use as much as you want, you'll have plenty of it. The only thing to worry about is screwing up the moisture content. If you use too much you'll end up with overly wet substrate. You also don't want a lot of sugars in your substrate as that will encourage contamination when you fruit them.
It's better to use the minimum required, but if you want faster colonization then use a little more and spread it around as much as possible.
Your minimum amount will depend on how thick your myc is in the LC. As long as you get a tiny bit of myc at each innoc site you'll do fine. 1/2cc per hole should be a pretty good amount to start with.
-FF
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smokedout420
The EnlightenedOne



Registered: 12/16/07
Posts: 484
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Re: LC Cloning - Newbie friendly pictorial tek! [Re: fastfred]
#8027920 - 02/15/08 03:59 PM (15 years, 11 months ago) |
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this kicks ass thanks for the write up
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jung
Stranger
Registered: 02/01/08
Posts: 19
Last seen: 14 years, 7 months
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Re: LC Cloning - Newbie friendly pictorial tek! [Re: smokedout420]
#8031101 - 02/16/08 12:47 PM (15 years, 11 months ago) |
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i'm gonna try this with shiitake.
but if you're shooting for exactly 4% with 4grams of honey, wouldn't 96ml of water is used instead of 100?
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jtbyrd
Stranger
Registered: 08/13/07
Posts: 12
Last seen: 15 years, 6 months
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Re: LC Cloning - Newbie friendly pictorial tek! [Re: jung]
#8182409 - 03/23/08 02:22 AM (15 years, 10 months ago) |
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Is that the lid separately wrapped in foil in the original picture? do you not leave it on the jar and wrap it in foil like you would if you were pcing a pf jar? just a bit confused....thanks for the help.
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ultralight
fury 762 where are you?


Registered: 03/20/05
Posts: 264
Last seen: 2 months, 23 days
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Re: LC Cloning - Newbie friendly pictorial tek! [Re: jtbyrd]
#8182542 - 03/23/08 04:03 AM (15 years, 10 months ago) |
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Quote:
jtbyrd said: Is that the lid separately wrapped in foil in the original picture? do you not leave it on the jar and wrap it in foil like you would if you were pcing a pf jar? just a bit confused....thanks for the help.
The pic of the lid alone, was just for reference, (a foil covered lid)... always keep the lid on the jar when sterilizing substrate!.
Edited by ultralight (03/23/08 04:14 AM)
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templeton1
loves mushrooms


Registered: 12/22/07
Posts: 410
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Re: LC Cloning - Newbie friendly pictorial tek! [Re: liamtheloser]
#8280010 - 04/13/08 10:28 PM (15 years, 9 months ago) |
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awesome tek, thanks
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Ozzy_shroomer
Mystery Machine



Registered: 02/10/08
Posts: 911
Loc: Summer All Year
Last seen: 5 years, 7 months
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Re: LC Cloning - Newbie friendly pictorial tek! [Re: templeton1]
#8518544 - 06/13/08 05:15 AM (15 years, 7 months ago) |
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Awsome tek i like it and think it should be brought back up for other to read..
also just a thought going down from the cap into the stem might be a good way to clone wild mushrooms
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