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OfflineRogerRabbitM
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Re: glove box arm holes [Re: Tippinthru]
    #5877023 - 07/19/06 09:08 AM (17 years, 8 months ago)

You guys are all missing the point totally. NOTHING inside a glovebox needs to be sterile except what comes into direct contact with your spawn. I've said for years that alcohol, Lysol, etc., need not be used to clean a glovebox. Soap and water is sufficient, unless you plan to dump your grains or brf onto the floor of the glovebox, in which case you have a lot of learning to do.

The problem working bare armed is that several thousand dead skin cells per hour fall off each arm. That is a fact of human metabolism. In fact, the overwhelming majority of 'dust' in a house or on the furniture is actually dead skin cells. If you work bare armed, those skin cells that flake off your arms now have a chance to fall by simple gravity into a jar or petri dish. With a freshly laundered long sleeved shirt, the shirt will catch the majority of those dead skin cells, thus protecting your project. The shirt does NOT need to be sterile, so please stop confusing the subject with this silly arguing.

I also have a box of a thousand tyvek wrist sleeves with elastic at both ends. As hyphae said, they're a better choice and I concur. However, most of you don't have those, so a long sleeved, fine weave shirt is the next best choice.

Remember, we as cultivators are the number one vector of contamination, so depending on what you're doing at the time, have a supply of latex gloves, hairnets, surgical(not dust) masks and alcohol, oust and soap and water. Iodine is excellent as a bactericide. Always wash your hands and arms, even if you wear gloves and a shirt or tyvek sleeves. Always brush your teeth and use mouthwash just before sterile work, and wash your hair in the shower you take just before beginning work, then use a hairnet in addition to that.

If people would just follow those simple things, their contamination problems would be reduced considerably.
RR


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Invisiblefastfred
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Re: glove box arm holes [Re: RogerRabbit]
    #5877048 - 07/19/06 09:28 AM (17 years, 8 months ago)

> The problem working bare armed is that several thousand dead skin cells per hour fall off each arm.

Luckily they're dead though, so they won't take over the dish or culture you're working on.

I don't see why several thousand sterilized skin cells would be worse than a thousand non-sterile cloth fibers.

It really isn't that much of an issue anyway IMHO. If gloves that cover up to the wrists aren't good enough then you're already failing somewhere.


-FF

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Offlineaccesstwo
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Re: glove box arm holes [Re: fastfred]
    #5877387 - 07/19/06 11:36 AM (17 years, 8 months ago)

slow but direct movements are the best as they reduce the flow of air while getting things done quickly, only leave things open for as long as they need to be open eg lids. would putting a rack in a glove box help, as the dust will settle on the bottom under the jars and such, rather then around the jars?

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Offlinehyphae
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Re: glove box arm holes [Re: 0003]
    #5877411 - 07/19/06 11:41 AM (17 years, 8 months ago)

Still air GB doesn't mean there is no air currents as we move around, I will recommend always wiping down the inside of the box beforehand with chlorox wipes then mist the air down essentially scrubbing the air inside after everything placed in the GB is also wiped down with chlorox wipes, this is all part of our individual sterile culture technique and reducing exposure to mold spore counts is the number one consideration for success. Skin cells are of little concern and will have really no affect IME and besides only our hands should really be anywhere close to any openings if things are being done properly. Nice debate so far.


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Getting the most out of your casings!, A pinning strategy.
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Not what is overlay but rather what overlay is
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"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

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Offlinehyphae
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Re: glove box arm holes [Re: accesstwo]
    #5877437 - 07/19/06 11:46 AM (17 years, 8 months ago)

Quote:

accesstwo said:
slow but direct movements are the best as they reduce the flow of air while getting things done quickly, only leave things open for as long as they need to be open eg lids. would putting a rack in a glove box help, as the dust will settle on the bottom under the jars and such, rather then around the jars?



Sterile culture technique has zero tolerance for dust there should be none in a GB. Theres none in mine except for the thousands of skin cells  falling off my arms :wink: sorry RR just had too :smirk: :blush:


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

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Offline0003
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Re: glove box arm holes [Re: hyphae]
    #5878327 - 07/19/06 04:28 PM (17 years, 8 months ago)

I like this debate. Roger, how do you acquire your tyvek sleeves? I am considering filling my glove box with smoke to monitor the airflow inside caused my normal arm movements. It would show if there are any strong currents.

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Invisiblefastfred
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Re: glove box arm holes [Re: 0003]
    #5878450 - 07/19/06 05:07 PM (17 years, 8 months ago)

Smoke will probably just smoke it up. Try a stick of insence. They produce a nice thin and easily visible streamer of smoke that doesn't smell foul.


-FF

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InvisiblePrisoner#1
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Re: glove box arm holes [Re: 0003]
    #5878506 - 07/19/06 05:25 PM (17 years, 8 months ago)

Quote:

0003 said:
I like this debate. Roger, how do you acquire your tyvek sleeves?




you can get painters cloths (coveralls) made of tyvek at home depot for about $5


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InvisibleOmnicracker
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Re: glove box arm holes [Re: Prisoner#1]
    #5878526 - 07/19/06 05:29 PM (17 years, 8 months ago)

badass! im getting one


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InvisiblePrisoner#1
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Re: glove box arm holes [Re: Omnicracker]
    #5878539 - 07/19/06 05:35 PM (17 years, 8 months ago)

goggles are a must for maximum geekiness

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InvisibleOmnicracker
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Re: glove box arm holes [Re: Prisoner#1]
    #5878554 - 07/19/06 05:40 PM (17 years, 8 months ago)

always use protection


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OfflineRogerRabbitM
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Re: glove box arm holes [Re: Prisoner#1]
    #5878587 - 07/19/06 05:51 PM (17 years, 8 months ago)

Quote:

Prisoner#1 said:
goggles are a must for maximum geekiness




I wear trifocals because I'm old. Do they count?
RR


--------------------
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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Offlinehyphae
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Re: glove box arm holes [Re: Prisoner#1]
    #5878623 - 07/19/06 06:02 PM (17 years, 8 months ago)

Quote:

Prisoner#1 said:
Quote:

0003 said:
I like this debate. Roger, how do you acquire your tyvek sleeves?




you can get painters cloths (coveralls) made of tyvek at home depot for about $5





Just like looking in the mirror except that mine fits much tighter around the arms (especially the biceps) and chest as well as the legs  :tongue: :smirk: :thumbup:


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

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InvisiblePrisoner#1
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Re: glove box arm holes [Re: Omnicracker]
    #5878626 - 07/19/06 06:03 PM (17 years, 8 months ago)

Quote:

Omnicracker said:
always use protection





my wife kept a stun gun under the pillow to prevent pregnancy... it usualy worked

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InvisiblePrisoner#1
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Re: glove box arm holes [Re: hyphae]
    #5878629 - 07/19/06 06:05 PM (17 years, 8 months ago)

Quote:

hyphae said:
Just like looking in the mirror except that mine fits much tighter around the arms (especially the biceps) and chest as well as the legs  :tongue: :smirk: :thumbup:




I had a roomie that borrowed my t-shirts to make him look more muscular  :smirk:

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