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Omnicracker
Crusted Trolltivator

Registered: 09/18/05
Posts: 1,421
Loc: Wal-Merica
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GloveBox Design Help
#5873356 - 07/18/06 01:08 PM (17 years, 6 months ago) |
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After a few eyeopening experiences with contams and lousy Multispore cultures, Ive decided to develop my agar skills and work on strain isolation. So im building a glovebox.
I got 3 of these marthas for $3 each last week.

i altered the frame of the third to a size more fitting to a glove box.

so before i start chopping and ruin a perfectly good martha i want to get a few things straight. Do i need to worry about contams/air pulling in through the fabric on the zipper while im working in the GB? i see now why it would be smart not to have flexible walls the shift the pressure/currents in the box. all i could think of was to run a piece of duct tape over the zipper after i close it, but this sounds like it might be a pain in the ass with repeated use.
should i not use this zippered design for my GB, i still have a 30 Gal Tub that i originally planned on using, but i like this one more.
any ideas?
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L0ki
Stranger
Registered: 06/22/06
Posts: 31
Last seen: 16 years, 7 months
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Re: GloveBox Design Help [Re: Omnicracker]
#5873456 - 07/18/06 01:38 PM (17 years, 6 months ago) |
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I wouldnt use that IMHO. I tried one of those once and nothing but problems. Everyime you move you can see the walls bloating and pulling in and that only means one thing.... Depends also where you use it too but, that defeats the purpose of a glovebox. I've had better luck in the past with a 110 lt. tub and a sheet of glass. Id load the tub, spray in my lysol, close it for 1 min. Then id slide the glass back enough to stick my arms in (cleaned and gloved of course) and do everything fast. not foolproof but cheap enough for a 99% success rate with agar.
But what you could do is seal off the zippers and cut out a mag sealed door using a small piece of plexi and some magnetic tape. I've seen this done before. better than the zippers but not the greatest
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Omnicracker
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Registered: 09/18/05
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Re: GloveBox Design Help [Re: L0ki]
#5873504 - 07/18/06 01:48 PM (17 years, 6 months ago) |
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thats what i thought, the flexible walls really seemed like a bad idea.
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L0ki
Stranger
Registered: 06/22/06
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Re: GloveBox Design Help [Re: Omnicracker]
#5873574 - 07/18/06 02:00 PM (17 years, 6 months ago) |
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well see, the flex walls are only part of the prob. its the fact that there somewhere for air to rush in and out. the walls only magnify that problem. But if a seal is devised, the walls can flex all they want, so long as the air doesn't exchange. What you need for this design is a solid entrance that doesn't allow air in/out, hence the zipper problem. magseals can work, so can tape, but a proper job would be one, solid unit with gloverings. Like the professional kind.
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Tippinthru
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Registered: 04/07/05
Posts: 1,131
Loc: "The Garden"...
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Re: GloveBox Design Help [Re: Omnicracker]
#5873613 - 07/18/06 02:07 PM (17 years, 6 months ago) |
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Having cloth on it, will on occasion cause it to act like a bellows. Which you do NOT want.
You can buy a 4x8 sheet of thin kitchen type wall board (like Formica) at Home Depot, etc, for around $20. It is rigid, easy to cut, and work with.
-------------------- Perfection is attained by slow degrees; it requires the hand of time... [
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Roadkill
Retired Shroomery Mod


Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: GloveBox Design Help [Re: Omnicracker]
#5873648 - 07/18/06 02:13 PM (17 years, 6 months ago) |
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-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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Tippinthru
contented

Registered: 04/07/05
Posts: 1,131
Loc: "The Garden"...
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Re: GloveBox Design Help [Re: Roadkill]
#5873950 - 07/18/06 03:29 PM (17 years, 6 months ago) |
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Road,
Yours is way cool, portable & effective.
I have the use of a monster one, but want my own.
Below is my basic plan.

 Using these.
Object of the filter patch is to allow gas exchange & evaporation.
-------------------- Perfection is attained by slow degrees; it requires the hand of time... [
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Omnicracker
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Registered: 09/18/05
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Re: GloveBox Design Help [Re: Tippinthru]
#5874016 - 07/18/06 03:56 PM (17 years, 6 months ago) |
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time to reformulate... thanks for help!
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RogerRabbit
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Registered: 03/26/03
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Re: GloveBox Design Help [Re: Omnicracker]
#5874330 - 07/18/06 05:37 PM (17 years, 6 months ago) |
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I have tyvek sleeves instead of gloves installed in my glovebox so I can remove my hands as needed to keep the alcohol lamp on the outside. I use the tyvek sleeves and latex gloves.
Whatever you set up, just remember to keep the air inside still. If you use sleeves like I do instead of installed gloves, be sure to shut off all fans, AC units, etc., that could cause drafts in the room. Soap and water is good enough to clean the box. I don't use flammable materials in mine. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Omnicracker
Crusted Trolltivator

Registered: 09/18/05
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Re: GloveBox Design Help [Re: RogerRabbit]
#5874412 - 07/18/06 06:02 PM (17 years, 6 months ago) |
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RR needs to write a book about all the things his brain knows. mushroom relaed things of course.
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fastfred
Old Hand



Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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Re: GloveBox Design Help [Re: Omnicracker]
#5876221 - 07/19/06 12:52 AM (17 years, 6 months ago) |
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> RR needs to write a book about all the things his brain knows.
I think he's making a video series. Looks like it should be pretty cool.
As far as the gloveboxes... With the amount of effort you guys seem to be putting in you could just as easily build a flow hood. Flow hoods are SO much better and nicer to use.
You can do agar work just fine in open air with maybe a 10% contam rate. If you're going to put much effort into something though, you might as well make a real flowhood. I made one years ago with a $40 wallyworld hepa that worked great. If you want a cheap solution, just get a ~$15-20 replacement hepa filter from the store, cut a hole in a plastic bin that will fit the filter, glue the filter in place, then hook up a blower to run air through it. If you can find a scrap blower to cannibalize and have some building materials around you can do the whole project for the cost of a replacement hepa filter (~$20).
-FF
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Tippinthru
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Registered: 04/07/05
Posts: 1,131
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Re: GloveBox Design Help [Re: fastfred]
#5876246 - 07/19/06 01:07 AM (17 years, 6 months ago) |
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Quote:
fastfred said: > As far as the gloveboxes... With the amount of effort you guys seem to be putting in you could just as easily build a flow hood. Flow hoods are SO much better and nicer to use.
You can do agar work just fine in open air with maybe a 10% contam rate. If you're going to put much effort into something though, you might as well make a real flowhood. I made one years ago with a $40 wallyworld hepa that worked great. If you want a cheap solution, just get a ~$15-20 replacement hepa filter from the store, cut a hole in a plastic bin that will fit the filter, glue the filter in place, then hook up a blower to run air through it. If you can find a scrap blower to cannibalize and have some building materials around you can do the whole project for the cost of a replacement hepa filter (~$20). -FF
>>>> You can do agar work just fine in open air with maybe a 10% contam rate.<<<<<
More like 90% contam rate
>>>>>>If you want a cheap solution, just get a ~$15-20 replacement hepa filter from the store, cut a hole in a plastic bin that will fit the filter, glue the filter in place, then hook up a blower to run air through it. <<<<<
That is NOT a laminar flowhood.
You get what you pay for.
That especially holds true, building a LAMINAR FLOW HOOD.
-------------------- Perfection is attained by slow degrees; it requires the hand of time... [
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fastfred
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Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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Re: GloveBox Design Help [Re: Tippinthru]
#5876374 - 07/19/06 02:28 AM (17 years, 6 months ago) |
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> More like 90% contam rate
I've done thousands of cultures and petri dishes in the open air with nothing but a bunsen burner and a loop. It's not that hard. If I actually had a 10% contam rate I'd be damn pissed, I just said 10% because that's probably about what most people can get at home with a little effort and practice.
Read up on aseptic and sterile technique. You should have no problem getting 75% success with a little effort. 90%+ is not that hard with some practice and the right environment (no drafts and a bunsen burner).
> That is NOT a laminar flowhood.
Never said it was a LAMINAR flow hood. You don't need a laminar flow if it's designed right. It should be made like a fume hood operating in reverse. All you need is a source of clean air and an opening that isn't too big for your airflow.
Something like this will work just fine.


Those aren't my pics, they're Suntzu's. I would do things a little differently, but I'm sure this design works well enough. I posted the pics in this thread if you want to see it debated.
I know there are some naysayers that insist that you need laminar flow, static pressure, CFMs, and all sorts of rigamarole. That may be true if you insist on a full sized opening and laminar flow, but if you just want a plain old flow hood that works, then you don't need to worry about all that stuff. I've had perfect results with a similar design and several other people have posted in various threads saying they've had success with similar designs.
I've been meaning to make another one and post a tek on it, but I can't even afford the cost of the materials at the moment. If someone wants more info I would be happy to provide more details on my designs.
-FF
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