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OfflineInner_Chi
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Registered: 11/17/04
Posts: 141
Last seen: 6 years, 9 months
need tips concerning WBS tek in spawn bags
    #5871438 - 07/17/06 10:40 PM (17 years, 6 months ago)

so here's our story in a nutshell. this is the third time we've tried using WBS, the first time was in jars, the last time was in mycobags and both times we had mold contamination. so we figured out that we might not have rinsed the seed well enough and also didn't pressure cook the seed long enough.

the seed looks dark brown this time, which is different from anything i've seen in the online pictures. we pressure cooked the bags three times each and made sure the seed was well drained.

there is no signs of contamination thus far. there is a slimy substance growing around the outside of the bags, and we've seen this same substance in the past tries. we're hoping that is the start of the myccellium, and that before it just didn't take off due to the mold.

any advice?

FYI- we sterilized the area for inoculation using rubbing alcohol and wore rubber gloves and used oil candles to clean the air. the seringe was re-sterilized after each injection as well. so all SHOULD be well in that area as far as i know.


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if you think you are better than me, you should speculte less and enquire;if you know you are better than me then i have already won. if truely you are better then your example will teach me without an attempt to do so.


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OfflineAlphabetSoup
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Registered: 07/17/06
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Re: need tips concerning WBS tek in spawn bags [Re: Inner_Chi]
    #5871638 - 07/17/06 11:27 PM (17 years, 6 months ago)

there really isn't enough information to tell you where you are going
wrong, but you need only pressure cook once!

a slimy substance is bad, myc is either fluffy white or strandy white.
the slimy stuff is probably a bacterial contamination.

if you are having trouble inoculating in open air maybe you should
consider making a glove box? it is certainly possible to have success
in open air however some enviroments are just unforgiving. if you have
alot of fabric/carpet in the room you are inoculating in it will be
harder.

chances are you are over looking something small but important, attention to detail is very important


Edited by AlphabetSoup (07/17/06 11:29 PM)


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OfflineCapless
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Registered: 06/25/06
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Re: need tips concerning WBS tek in spawn bags [Re: Inner_Chi]
    #5871658 - 07/17/06 11:33 PM (17 years, 6 months ago)

I was recommended 1 rense, a 24-48h soak in a warm coffee solution w/ gypsm, and another rense. Rense both times till the water runs clear. I just plug my drain and let it pool to see how clear it is. It doesn't take me that long to do about 5 - 6 quarts in my bathroom :P Shouldn't take you long at all.

Drain your seeds in a close-knit wire strainer. Drain for at least 10 minutes each batch. If you have a large batch tilt it back and forth to dump pooled water out. Trust me, there is some in there. Give it the shakes and load it into whatever.

Cook for the intended time. I did 1hour. For a 2 & 1/2 Quart bag and a 3 & 1/2 Quart bag.

Innoced with spores, and they are rapidly growing right now with no signs of contam!

All this courtesy of my mentor Monstermitch.


--------------------
http://www.toolband.com/
"... This body holding me reminds me of my own mortality.
Embrace this moment. Remember, We are eternal.
all this pain is an illusion...."


Edited by Capless (07/17/06 11:34 PM)


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OfflineInner_Chi
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Re: need tips concerning WBS tek in spawn bags [Re: Capless]
    #5872277 - 07/18/06 03:28 AM (17 years, 6 months ago)

Thanks guys for the speedy advice... i can and probably will take the advice on a glove box since as of recently i have my dog runnin around the house... will he make a difference as far as open air contamination?

I believe i rinse well enough... made a strainer out of a plastic garden tray with the holes in it and some window screen.

Also i use a pressure cooker with a dial on it ranging from 200 to somethin like 450... We did them for an hour at 350 is that too much or should i do a lower heat for slower durration? most people have the PCs that u throw on the stove so i have never heard the temp mentioned...

Thanks again for that advice, dualy noted!

-Chi


--------------------
if you think you are better than me, you should speculte less and enquire;if you know you are better than me then i have already won. if truely you are better then your example will teach me without an attempt to do so.


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Offlinethenewguy05
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Re: need tips concerning WBS tek in spawn bags [Re: Inner_Chi]
    #5872536 - 07/18/06 06:43 AM (17 years, 6 months ago)

sounds like you have wet spot bacteria. wet spot is usually caused by excess moisture . before you sterilize, next time add a few handfulls of vermiculite in the bag or you could start swabbing the grain with a dry paper towel before you place the grain in a bag and sterilize.

if it's not wetspot a... pic would help


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OfflineCapless
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Re: need tips concerning WBS tek in spawn bags [Re: thenewguy05]
    #5872565 - 07/18/06 07:02 AM (17 years, 6 months ago)

Quote:

thenewguy05 said:
sounds like you have wet spot bacteria. wet spot is usually caused by excess moisture . before you sterilize, next time add a few handfulls of vermiculite in the bag or you could start swabbing the grain with a dry paper towel before you place the grain in a bag and sterilize.

if it's not wetspot a... pic would help




That has me scared about 2 recent bags I noc'd up. They are visibly more moist than my first bags(at least if I remember correctly) and I think they might fall to contams, but we can always hope for the best, ya know?


--------------------
http://www.toolband.com/
"... This body holding me reminds me of my own mortality.
Embrace this moment. Remember, We are eternal.
all this pain is an illusion...."


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InvisibleTippinthru
contented

Registered: 04/07/05
Posts: 1,131
Loc: "The Garden"...
Re: need tips concerning WBS tek in spawn bags [Re: Inner_Chi]
    #5872715 - 07/18/06 08:21 AM (17 years, 6 months ago)

What size filter patch bag, how much WBS are you putting in it, and how are you sealing it?

If you are placing 5 or 6 quarts in each bag?

And, PC'ing several at once together?

You need to up your PC time to around 3 hours.

You also don't want any WBS in contact with the bag filter.


--------------------
Perfection is attained by slow degrees; it requires the hand of time...
[


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OfflineInner_Chi
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Registered: 11/17/04
Posts: 141
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Re: need tips concerning WBS tek in spawn bags [Re: Capless]
    #5874366 - 07/18/06 05:47 PM (17 years, 6 months ago)


:mushroom2:


--------------------
if you think you are better than me, you should speculte less and enquire;if you know you are better than me then i have already won. if truely you are better then your example will teach me without an attempt to do so.


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OfflineInner_Chi
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Registered: 11/17/04
Posts: 141
Last seen: 6 years, 9 months
Re: need tips concerning WBS tek in spawn bags [Re: Inner_Chi]
    #5874384 - 07/18/06 05:52 PM (17 years, 6 months ago)

we've been putting about 6 8 ounce cups in each bag, we have some regular bags without the self sealer that we are using hot glue to plug the hole, and we've got some self sealing bags as well.

we PCed each bag one at a time on 350 degrees for an hour and a half. is that too hot, and how the duration?

i'm guessing that we still didn't get the seed drained well enough. my hopes are that this could still work though, getting slightly discouraged at the waste rate of our spores.


--------------------
if you think you are better than me, you should speculte less and enquire;if you know you are better than me then i have already won. if truely you are better then your example will teach me without an attempt to do so.


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OfflineInner_Chi
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Registered: 11/17/04
Posts: 141
Last seen: 6 years, 9 months
Re: need tips concerning WBS tek in spawn bags [Re: Inner_Chi]
    #5874392 - 07/18/06 05:54 PM (17 years, 6 months ago)

what exactly is gypsm?


--------------------
if you think you are better than me, you should speculte less and enquire;if you know you are better than me then i have already won. if truely you are better then your example will teach me without an attempt to do so.


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