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OfflineToxxy
Random Gary
Registered: 07/07/06
Posts: 36
Last seen: 16 years, 10 months
LC troubles
    #5870723 - 07/17/06 07:41 PM (17 years, 6 months ago)

I inoculated my LC on Friday, and since Sunday afternoon, all I've seen have been little tiny things that look like breadcrumbs, only a fraction of the size. They have been incubating at 80F, with foil tightly wrapped over the lid on the top of the jar to make sure no air gets in or out. Is this normal? I know it's a patience game, but I've seen no progress whatsoever over the past three days. The LC tek said that I could expect the jars to be fully colonized after about a week; I'm half-way to what should be gametime, and I don't look even an 1/8 the way there.

I'm beginning to think maybe I messed up on the inoculation. How do you guys assemble the syringe? I took the pink plastic piece out of the tip of the handle-piece, and left the blue plastic piece in the needle-piece. I just screw that part in, right?

Thanks in advance for any help. As we speak, I'm starting another LC just to be safe.


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Offlineaccesstwo
Stranger
Registered: 04/29/06
Posts: 201
Last seen: 12 years, 4 months
Re: LC troubles [Re: Toxxy]
    #5870780 - 07/17/06 07:50 PM (17 years, 6 months ago)

dont know what your talking about for the syrine part, but to put together a syringe, you should screw on the needle to the body then push the plunger into the syringe body, then put on the cap, wrap in foil and pc to kill any spores that might have been in the syringe.

any growth is good growth, you lc needs air, you should try shaking the jar to airate(dont if you have polyfil or tyvek installed)


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OfflineToxxy
Random Gary
Registered: 07/07/06
Posts: 36
Last seen: 16 years, 10 months
Re: LC troubles [Re: accesstwo]
    #5870873 - 07/17/06 08:10 PM (17 years, 6 months ago)

Quote:

accesstwo said:
any growth is good growth, you lc needs air, you should try shaking the jar to airate(dont if you have polyfil or tyvek installed)




Are you saying I should take the foil off the top and expose the injection site to the air, or just shake it around with the foil still in place & my finger over the hole?


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Invisiblegibiceh
Mudvayne

Registered: 05/19/06
Posts: 73
Re: LC troubles [Re: Toxxy]
    #5871092 - 07/17/06 09:02 PM (17 years, 6 months ago)

Don't take the foil off and expose it to open air. You should be able to shake it just fine with the foil still in place and your finger over the hole.


--------------------

Are you feeling happy?


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OfflineToxxy
Random Gary
Registered: 07/07/06
Posts: 36
Last seen: 16 years, 10 months
Re: LC troubles [Re: gibiceh]
    #5871565 - 07/17/06 11:13 PM (17 years, 6 months ago)

The honey I used was sort of cloudy at the bottom and had some white stuff on the top of it. Would that slow my LC down, or does that just raise the likelihood of contamination?


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InvisibleLiquidkick
H2O
Registered: 05/03/02
Posts: 2,635
Re: LC troubles [Re: Toxxy]
    #5871798 - 07/18/06 12:08 AM (17 years, 6 months ago)

are you really questioning the point of assembling a syringe?

Take need out of sterile package.

with the cap on it. screw it on to the syringe and viola. Make sure you dipped your hands in alcohol and rubbed them up and dried them.

Clean is the key.

Then you make your LC, and let cool, inject ~1-2Ml of spore solution. You have a marble or something in the LC before you cooked it up right? Now swirl it around.

as for the hole, put some micropore tape over it and lose the foil.

when you swirl try not to get the micropore wet.

sometimes it takes a bit longer for LC, but if you don't have anything within a week its probably toast.


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