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solace1993
Stranger
Registered: 07/06/06
Posts: 9
Last seen: 17 years, 4 months
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Can you use regular table sugar?
#5870228 - 07/17/06 06:14 PM (17 years, 6 months ago) |
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Can you use it with the potatoe agar recipe?
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Tippinthru
contented

Registered: 04/07/05
Posts: 1,131
Loc: "The Garden"...
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Re: Can you use regular table sugar? [Re: solace1993]
#5870235 - 07/17/06 06:16 PM (17 years, 6 months ago) |
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It is not advisable. If it worked well, everyone would use it. They don't. That should tell you something.
-------------------- Perfection is attained by slow degrees; it requires the hand of time... [
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Roadkill
Retired Shroomery Mod


Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: Can you use regular table sugar? [Re: solace1993]
#5870245 - 07/17/06 06:19 PM (17 years, 6 months ago) |
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I'd use honey if I was you.
-PDYA-
500ml water 5 grams of instant potato flakes(Potato Buds Brand - I use) 9 grams agar agar 10 ml honey (Raw natural pure honey - Beemaid Brand - I use) 1 gram of Nutritional Yeast (optional)
tc
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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fastfred
Old Hand



Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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Re: Can you use regular table sugar? [Re: Roadkill]
#5870288 - 07/17/06 06:29 PM (17 years, 6 months ago) |
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> Can you use it with the potatoe agar recipe?
No, use corn sugar (dextrose).
Potato Dextrose Agar (PDA)(FDA M127) ------------------------------------ 200 g Potato infusion [dilute to 1L total, ~4g solids] 20 g Dextrose 20 g Agar 1 L Distilled water (dH2O)
To prepare potato infusion, boil 200 g scrubbed, sliced(unpeeled) potatoes in 1 liter distilled water for 30 min. Filter through cheesecloth, saving effluent, which is potato infusion (or use commercial dehydrated form). Mix in other ingredients and boil to dissolve. [Dilute to obtain 1 L final volume] Autoclave 15 min at 121°C. Dispense 20-25 ml portions into sterile 15 x 100 mm petri dishes. Final pH, 5.6 ± 0.2.
Medium should not be re-melted more than once. Medium powder is available commercially but may require supplementing with extra agar to a final concentration of 20 g/liter. To BBL or Difco dehydrated medium, add 5 g of agar.
The broth is clear to slightly opalescent and yellowish in color. [1][2]
Edited by fastfred (07/17/06 06:45 PM)
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cappa
Nerd
Registered: 02/12/06
Posts: 854
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Re: Can you use regular table sugar? [Re: fastfred]
#5870319 - 07/17/06 06:36 PM (17 years, 6 months ago) |
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Quote:
fastfred said:
PDA doesn't call for any sugar. Potato Dextrose Agar (PDA)(FDA M127) ------------------------------------ 20 g Dextrose
Dextrose is a kind of sugar.
That said, it's a simple sugar, unlike table sugar or white sugar. I'd put my money that you can use corn sugar from a brewing store in it's place. I've never done it, but it sounds good in theory. 
GL!
-------------------- Their are 10 types of people. Those that understand binary, and those who don't. ~Cappa.
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fastfred
Old Hand



Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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Re: Can you use regular table sugar? [Re: cappa]
#5870383 - 07/17/06 06:48 PM (17 years, 6 months ago) |
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Good catch cappa. Silly me. That will teach me to read what I post. 5 shrooms for you!
That must also be why my last batch of PDA didn't work very well! 
Alright, everyone laugh at me now because you probably won't get another chance for a long while.
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Roadkill
Retired Shroomery Mod


Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: Can you use regular table sugar? [Re: fastfred]
#5870747 - 07/17/06 07:44 PM (17 years, 6 months ago) |
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-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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Tippinthru
contented

Registered: 04/07/05
Posts: 1,131
Loc: "The Garden"...
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Re: Can you use regular table sugar? [Re: cappa]
#5870783 - 07/17/06 07:51 PM (17 years, 6 months ago) |
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>>>>>>I'd put my money that you can use corn sugar from a brewing store in it's place. I've never done it, but it sounds good in theory. <<<<<
Dextrose IS corn sugar. It is no theory, dry corn sugar from a beer brew store works perfectly.

http://cgi.ebay.com/ws/eBayISAPI.dll?ViewItem&item=4464129031&rd=1&sspagename=STRK%3AMEWA%3AIT&rd=1
-------------------- Perfection is attained by slow degrees; it requires the hand of time... [
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Omnicracker
Crusted Trolltivator

Registered: 09/18/05
Posts: 1,421
Loc: Wal-Merica
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Re: Can you use regular table sugar? [Re: Tippinthru]
#5870830 - 07/17/06 08:00 PM (17 years, 6 months ago) |
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^^^^ that LC has Light Malt Extract in it, along with dextrose, it should be noted.
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Amatoxin
Injected With A Poison


Registered: 03/27/05
Posts: 1,934
Loc: Not So Great Britain
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Re: Can you use regular table sugar? [Re: solace1993]
#5870871 - 07/17/06 08:10 PM (17 years, 6 months ago) |
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Quote:
solace1993 said: Can you use it with the potatoe agar recipe?
Yes you can. I used to use peroxidated PSYA all the time with good results
--------------------
Sectioned Under The Mental Health Act Sat 20-10-07 to Thurs 01-11-07 for playing TECHNO music
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Tippinthru
contented

Registered: 04/07/05
Posts: 1,131
Loc: "The Garden"...
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Re: Can you use regular table sugar? [Re: Roadkill]
#5870968 - 07/17/06 08:32 PM (17 years, 6 months ago) |
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ha ha ha ha he he he he!
-------------------- Perfection is attained by slow degrees; it requires the hand of time... [
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Roadkill
Retired Shroomery Mod


Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: Can you use regular table sugar? [Re: Tippinthru]
#5871018 - 07/17/06 08:43 PM (17 years, 6 months ago) |
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muhahahahaaaaaa
might be my last chance to razz him...
so I'm enjoying it!~
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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Liquidkick
H2O
Registered: 05/03/02
Posts: 2,635
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Re: Can you use regular table sugar? [Re: Roadkill]
#5873699 - 07/18/06 02:24 PM (17 years, 6 months ago) |
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whos razzing who? I want in.
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