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Offlinesolace1993
Stranger
Registered: 07/06/06
Posts: 9
Last seen: 17 years, 4 months
Can you use regular table sugar?
    #5870228 - 07/17/06 06:14 PM (17 years, 6 months ago)

Can you use it with the potatoe agar recipe?


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InvisibleTippinthru
contented

Registered: 04/07/05
Posts: 1,131
Loc: "The Garden"...
Re: Can you use regular table sugar? [Re: solace1993]
    #5870235 - 07/17/06 06:16 PM (17 years, 6 months ago)

It is not advisable.
If it worked well, everyone would use it.
They don't. That should tell you something.


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Perfection is attained by slow degrees; it requires the hand of time...
[


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InvisibleRoadkillM
Retired Shroomery Mod
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Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: Can you use regular table sugar? [Re: solace1993]
    #5870245 - 07/17/06 06:19 PM (17 years, 6 months ago)

I'd use honey if I was you.

-PDYA-

500ml water
5 grams of instant potato flakes(Potato Buds Brand - I use)
9 grams agar agar
10 ml honey (Raw natural pure honey - Beemaid Brand - I use)
1 gram of Nutritional Yeast (optional)


tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Invisiblefastfred
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Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
Re: Can you use regular table sugar? [Re: Roadkill]
    #5870288 - 07/17/06 06:29 PM (17 years, 6 months ago)

> Can you use it with the potatoe agar recipe?

No, use corn sugar (dextrose).



Potato Dextrose Agar (PDA)(FDA M127)
------------------------------------
200 g Potato infusion [dilute to 1L total, ~4g solids]
20 g Dextrose
20 g Agar
1 L Distilled water (dH2O)

To prepare potato infusion, boil 200 g scrubbed, sliced(unpeeled) potatoes in 1 liter distilled water for 30 min. Filter through cheesecloth, saving effluent, which is potato infusion (or use commercial dehydrated form). Mix in other ingredients and boil to dissolve. [Dilute to obtain 1 L final volume] Autoclave 15 min at 121°C. Dispense 20-25 ml portions into sterile 15 x 100 mm petri dishes. Final pH, 5.6 ± 0.2.

Medium should not be re-melted more than once. Medium powder is available commercially but may require supplementing with extra agar to a final concentration of 20 g/liter. To BBL or Difco dehydrated medium, add 5 g of agar.

The broth is clear to slightly opalescent and yellowish in color. [1][2]


Edited by fastfred (07/17/06 06:45 PM)


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Invisiblecappa
Nerd
Registered: 02/12/06
Posts: 854
Re: Can you use regular table sugar? [Re: fastfred]
    #5870319 - 07/17/06 06:36 PM (17 years, 6 months ago)

Quote:

fastfred said:

PDA doesn't call for any sugar.
Potato Dextrose Agar (PDA)(FDA M127)
------------------------------------
20 g Dextrose 





Dextrose is a kind of sugar.

That said, it's a simple sugar, unlike table sugar or white sugar.
I'd put my money that you can use corn sugar from a brewing store in it's place. I've never done it, but it sounds good in theory.  :smile:


GL!


--------------------
Their are 10 types of people. Those that understand binary, and those who don't.
~Cappa.


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Invisiblefastfred
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Re: Can you use regular table sugar? [Re: cappa]
    #5870383 - 07/17/06 06:48 PM (17 years, 6 months ago)

Good catch cappa.  Silly me.  That will teach me to read what I post.  5 shrooms for you!

That must also be why my last batch of PDA didn't work very well! :confused:

Alright, everyone laugh at me now because you probably won't get another chance for a long while. :blush:


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InvisibleRoadkillM
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Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: Can you use regular table sugar? [Re: fastfred]
    #5870747 - 07/17/06 07:44 PM (17 years, 6 months ago)

:evil:

:hehehe:

:dumblol:

:voodoo:


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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InvisibleTippinthru
contented

Registered: 04/07/05
Posts: 1,131
Loc: "The Garden"...
Re: Can you use regular table sugar? [Re: cappa]
    #5870783 - 07/17/06 07:51 PM (17 years, 6 months ago)

>>>>>>I'd put my money that you can use corn sugar from a brewing store in it's place. I've never done it, but it sounds good in theory. <<<<<

Dextrose IS corn sugar. It is no theory, dry corn sugar from a beer brew store works perfectly.


http://cgi.ebay.com/ws/eBayISAPI.dll?ViewItem&item=4464129031&rd=1&sspagename=STRK%3AMEWA%3AIT&rd=1


--------------------
Perfection is attained by slow degrees; it requires the hand of time...
[


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InvisibleOmnicracker
Crusted Trolltivator

Registered: 09/18/05
Posts: 1,421
Loc: Wal-Merica
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Re: Can you use regular table sugar? [Re: Tippinthru]
    #5870830 - 07/17/06 08:00 PM (17 years, 6 months ago)

^^^^
that LC has Light Malt Extract in it, along with dextrose, it should be noted.


--------------------




xxx..Learn Something..xxx


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InvisibleAmatoxin
Injected With A Poison

Folding@home Statistics
Registered: 03/27/05
Posts: 1,934
Loc: Not So Great Britain
Re: Can you use regular table sugar? [Re: solace1993]
    #5870871 - 07/17/06 08:10 PM (17 years, 6 months ago)

Quote:

solace1993 said:
Can you use it with the potatoe agar recipe?




Yes you can. I used to use peroxidated PSYA all the time with good results :thumbup:


--------------------




Sectioned Under The Mental Health Act Sat 20-10-07 to Thurs 01-11-07 for playing TECHNO music


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InvisibleTippinthru
contented

Registered: 04/07/05
Posts: 1,131
Loc: "The Garden"...
Re: Can you use regular table sugar? [Re: Roadkill]
    #5870968 - 07/17/06 08:32 PM (17 years, 6 months ago)

ha ha ha ha he he he he! :laugh:


--------------------
Perfection is attained by slow degrees; it requires the hand of time...
[


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InvisibleRoadkillM
Retired Shroomery Mod
Male User Gallery

Registered: 12/11/01
Posts: 22,674
Loc: Montana
Trusted Cultivator
Re: Can you use regular table sugar? [Re: Tippinthru]
    #5871018 - 07/17/06 08:43 PM (17 years, 6 months ago)

muhahahahaaaaaa


might be my last chance to razz him...

so I'm enjoying it!~

:smirk:


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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InvisibleLiquidkick
H2O
Registered: 05/03/02
Posts: 2,635
Re: Can you use regular table sugar? [Re: Roadkill]
    #5873699 - 07/18/06 02:24 PM (17 years, 6 months ago)

whos razzing who? I want in.


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