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Blutjager
Inhuman


Registered: 06/11/06
Posts: 9,220
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93.7% ??
#5868116 - 07/17/06 01:40 AM (17 years, 6 months ago) |
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Three nights ago I shot up some jars and today I went in incubator to have a peek at them and nothing,no contamination(A first for me) But no growth either.I usually use LC but since everything I ever have even attempted to grow gets green I decided to just be simple and use the syringes I just received making sure to flame and alcohol wipe after every needle,I did this under a upside down fish tank like I saw on some post here a while back.I Don't have a glove box so I figured at least the air in there would be still and I sprayed alcohol and oust in there before I started working.Anyway I looked at the thermometer in the incubator and low and behold 93.7 deg.Is this why there is no growth.I mean I know I may just be impatient and 3 days is a little early to expect much with a multi-spore inoculation.I was really only checking to see if everything was green like it usually is(I swear I knock up jars and I can leave to go to the bathroom and they are green already when I get back) .Does having them at such a high temperature kill everything ?? I unplugged the heater as it is super hot in my house anyway and figure I will see what the temp is with it off after it goes down and than make adjustments accordingly.Is it cooked??
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hawksapprentice
Yearns to Snowboard


Registered: 06/06/03
Posts: 3,195
Loc: Oregon
Last seen: 8 months, 6 days
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93.7 isn't going to kill your spores, I think thermal death is somewhere around 133 degrees or so, not positive on that though. You should try to get your temperatures down into the mid 80s.
And yes you are being a little impatient. You should wait a week before becoming worried about your spores being non viable. Even then I have seen jars take two weeks before germination.
-------------------- "I celebrate the Earth, my home, my mother, my grave, and as long as men are Man they must, if they would preserve the integrated being, do the same---[and preserve]--this rank casual hungry smelly sweaty lusting transitory body, my oozy pulpy liquid-bag-swollen body, bones, blood, hair glands, my bejeweled sex; I love and celebrate it all. never to let men forget that they are animals as much as gods---that is one thing I shall say." Edward Abbey
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royer
±±±±±±±±±±

Registered: 05/15/06
Posts: 4,801
Loc: anywhere but here
Last seen: 4 years, 10 months
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Quote:
hawksapprentice said: 93.7 isn't going to kill your spores, I think thermal death is somewhere around 133 degrees or so, not positive on that though. You should try to get your temperatures down into the mid 80s.
And yes you are being a little impatient. You should wait a week before becoming worried about your spores being non viable. Even then I have seen jars take two weeks before germination.
i agree i have had jars that have taken 2 weeks to a month when i first started because my water content was a lil high
-------------------- ================================================= if you have any questions please feel free to pm me , thx :-)
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beatnicknick
The Innovator


Registered: 05/25/05
Posts: 1,074
Last seen: 12 years, 8 months
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Re: 93.7% ?? [Re: royer]
#5868260 - 07/17/06 04:49 AM (17 years, 6 months ago) |
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That temp is great for growing contams, and really great for stalling your jars, so yeah, keep it 80-85.
-------------------- I don't think for myself. I think as though I'm explaining my thoughts to someone else. I'm concerned only for those listening.
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darkbuster
Hometown Zero


Registered: 06/09/06
Posts: 43
Last seen: 8 years, 8 months
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i wouldn't worry about that too much. almost the exact same thing happened to me where i became impatient with the slowly raising temperature in my incubator, which is just a box sitting above my a/v reciever. so i threw some blankets over it, and over night the temperature shot up to nearly a hundred degress. i thought for sure i had ruined my jars, but i was able to bring the temp down to a steady 85 degrees, and after only a week the jars are doing just fine, with an average of about 40% colonization. of course as others have mentioned there is a much higher risk of contamination once you reach those extreme temperatures, but i have been fortunate enough not to encounter any yet, and hopefully you wont either.
well, good luck with the grow.
--------------------
Z-strain on BRF cake.
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toole
white-thumb (Onewhackmycophiliac)



Registered: 05/01/06
Posts: 500
Loc: spore #1203 - bas 2.34 - ...
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That is a very high temp to expose them to, likely to cause fermentation which will definitely halt all growth.
If you get your temps down to a solid 80-82 (which is optimal) you might have a chance to recover, just get them to a steady level and leave them alone ! For like a week and half, then check every few days for cobwebs or other contams.
Patience is just as important as any step to growing.
-------------------- -the adventures of suse and prescott.9- ..and the neverending triscut of doom !
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Blutjager
Inhuman


Registered: 06/11/06
Posts: 9,220
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Now its 87.5 deg.I'm actually surprised it went down that low considering how horribly hot it is in the whole house,Anyway thanks for the input,If the contam they contam,I'm used to that but it is good to be reassured that least I didn't kill them myself.
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Tippinthru
contented

Registered: 04/07/05
Posts: 1,131
Loc: "The Garden"...
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First, whats in the jars? Besides, hot spores that is. Are they PF style?
3 days is no big deal. Sometimes it takes a week, or more before you see spores germinate.
93/94F isn't good. But, by no means fatal.
You should test run an incubator, or grow chamber, about 3 days.
Before you use it.
That way, you know it works, or not.
That also allows you time to alter/adjust it, until you get it - where you want it.
I have been running my new one, 48 hours. Just tuning it. Because, I wanted to see how day/night cycles in temperatures, would affect it.
-------------------- Perfection is attained by slow degrees; it requires the hand of time... [
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Blutjager
Inhuman


Registered: 06/11/06
Posts: 9,220
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Half of them are cracked corn with a dash of verm and half are rye prepared using magdash's tek with the gypsum.Like I said I'm surprised they even went down that low(maybe just cause they are all alone in the dark).If 80-82 is what I'm looking for does anyone have any thoughts on how I can achieve this considering that it is so much hotter than that in the whole house ??
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AlphabetSoup
Stranger

Registered: 07/17/06
Posts: 29
Last seen: 17 years, 6 months
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the substrates temperature will be a little higher than the air around the jars. because it has only been 3 days you should be safe, but make sure you dont let the temp get that high anymore because once the spores germinate those high temps could certainly kill myc
Quote:
creamcorn said: mycelium dies at around 106, but cube spores can handle in the 150s for short periods of time.
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Tippinthru
contented

Registered: 04/07/05
Posts: 1,131
Loc: "The Garden"...
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In the hot summer, my exterior wall temperatures are 4 degree's hotter than the interior walls.
So, find a closet or cupboard, that has no contact with exterior walls & no sunlight that touches it (radiant heat). That will be about as cool as you can get without a swamp cooler, or AC.
-------------------- Perfection is attained by slow degrees; it requires the hand of time... [
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splifner180
Student


Registered: 03/24/06
Posts: 1,288
Loc: USA, East Coast
Last seen: 1 year, 3 months
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In my experience WBS, under optimal conditions, takes about 6-7 days to show signs of colonization from a multi-spore innoc. The temps you're at, while certainly not past any critical point, are high.
I would expect those temps to add a few days (3-5?) to the time to see colonization and yes, it will increase the liklihood of contams.
But frankly, it's hotter than a devil's rectum in my house this time of year and not only do I incubate at those temps, I'm often fruiting at them, too. It happens. It just takes longer.
splif
-------------------- First Grow: Ecuador -> LC -> HPoo/Straw -> Monotub Build a Do-It-Yourself Magnetic Stirrer in thirty minutes with no money.
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