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OfflineThe_Chariot
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Registered: 07/10/06
Posts: 127
Last seen: 13 years, 7 months
Any Suggestion? Please Help!!!!
    #5867560 - 07/16/06 10:00 PM (17 years, 6 months ago)

hello. I will be starting my first ever mushroom cultivation. From my reseaching, ive found alot of promblems that i will have. These are the steps I will be taking. Just wondering if this would work, and any suggestions would be much apperciated.
1. To sterilize the water i will be using a 10psi electric slow cooker. Of course I will have to boil the water longer since it doesnt go up to 15psi. When the water has cooled down I will begin to put the spore prints into the sterilized water.
2. The promblem is I dont have any fresh mushrooms to make prints and it is impossible to find any around here. What I'am going to do is take dried mushrooms and scrap out the insides of the caps and put that in the sterilized water and mix it around.
3. My next promblem is that I have a syringe but no needle. Then again it is still possible to suck up the water with the syringe itself. So thats what I will be doing (around 2ml) filled, and putting some kind of cover over the hole (where the needle is soppose to be) and letting it sit for the proper mount of time.
4. I have the jars, vermiculate, and brown rice, so there should be no flaws there. After mixing the ingredients and filling the jars, I will then take the syringe and apply the water into the jars. My question for this is, can it be layered on the top of the soil or does it have to be directly inserted with a needle so it can get farther down in the jars. If thats the case, I can take a clean utensil and move aside the substance to allow the solution in the syringe to reach further down in the jars.
5. The rest is time and patience, but thats the materials I have to work with.
Any suggestions? or will this be workable...


--------------------
My will be ashes and a casket for all I want. The apocalypse, this way comes. So come. Sing the song. Repent kingdom, the end is here and it won't remain silent. The devil is in Atlanta. The army is surrounded. The civilians panic.







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OfflinedeXtrous
complete tool
Male

Registered: 04/24/06
Posts: 1,743
Loc: Australia
Last seen: 9 months, 27 days
Re: Any Suggestion? Please Help!!!! [Re: The_Chariot]
    #5867997 - 07/17/06 12:26 AM (17 years, 6 months ago)

inoculate down the sides.

4 times.

0.25cc each.


Goodluck with your frankenstien grow.


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Offlinemrmonkey
Stranger

Registered: 07/03/06
Posts: 127
Last seen: 8 years, 1 month
Re: Any Suggestion? Please Help!!!! [Re: deXtrous]
    #5868081 - 07/17/06 01:16 AM (17 years, 6 months ago)

Skip the dry layer of Vermiculite, after you put the spores in the jar wrap a coffee filter around the lid and use a rubber band too secure it that will keep any contams out and allow air flow.

I have grown twice now and I am not the chemist when it comes to sterile, I have done it once following every tek and being super sterile and I have done it once just turning off the a/c and fans and not really being super sterile......I lost one jar out of 20 both times. BUT the last time I used a dry layer of vemi on top and it just confused me on if my jar is %100 colonized or not, being the vermi will not colonize like the rest of the jar.

If and when I write a TEK it will defiantly skip the dry layer of vemi and use a coffee filter on top of the jar, hence making a bigger cake which is what we all want!

I have learned one thing.....they, the shrooms are resilient lil bastards and they will grow....people who enjoy them are very thoughtful and make it seem like if you make a mistake here and their your screwed....it isn't so much that you are just that if something ugly pops up....don't mess with it, throw it away because possible death or very bad sickness is the worst result.

I also learned that if a jar/casing/cake does become contaminated you will defiantly know.....so don't spend so much time worrying about it because as you do worry your probably causing it.

Make sense? Hope so.....


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OfflineMLBjammer
Invitro Freak
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Registered: 02/24/06
Posts: 560
Loc: Southeastern USA
Last seen: 7 years, 14 days
Re: Any Suggestion? Please Help!!!! [Re: The_Chariot]
    #5868270 - 07/17/06 04:57 AM (17 years, 6 months ago)

You need to acquire at least a spore-print, preferably a syringe for your first attempt. Trying to inoculate with dry tissue will almost certainly fail. And a syringe w'out a needle is useless for this hobby. Go to any farm supply store and you can purchase blank syringes. As long as you don't live in CA, GA, or ID, any vendor will ship you prints or syringes in the US.
Contrary to the previous post, a dry vermiculite barrier is the best thing you can do to a PF jar--it is very effective at blocking contams and allowing gas exchange. A coffee filter will not function nearly as well. At least use a tyvek filter over the jar holes if you are growing in this manner.
You might as well steam sterilize if you cannot achieve 15 PSI.
And you need a needle to pierce the dry vermic. barrier--which should stay dry and relatively undisturbed.
Good luck.


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Invisiblefastfred
Old Hand
Male User Gallery

Folding@home Statistics
Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
Re: Any Suggestion? Please Help!!!! [Re: MLBjammer]
    #5869611 - 07/17/06 03:19 PM (17 years, 6 months ago)

> You might as well steam sterilize if you cannot achieve 15 PSI.

Nonsense, 10 psi will work just fine.


--------------------
It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy

The difference between the substances doesn't matter. This is a war on consciousness, on our right to the very essence of what we are. With no control over that, we have no need to speak of freedom or a free society. -fireseed

"If we are going to have a war on marijuana, the least we can do is pull the sick and the dying off the battlefield." -Neal Levine (MPP)

I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid


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