Trichoderma (or Penicilium / Aspergi) why? - Mushroom Cultivation

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aenima
member

Registered: 06/06/01
Posts: 194
Loc: UK
Last seen: 21 years, 2 months

Trichoderma (or Penicilium / Aspergi) why?

#586257 - 03/22/02 10:43 AM (21 years, 6 months ago)

been searching for pictures of the same contam i get all the time, and found some, which are included below (not my pictures, i take no credit etc etc).

It seems most think this is the contam Trichoderma , but i think it was anno who said ti is actually the contam Penicilium / Aspergi. Anyway, this is the ONLY contam i ever get, and have been getting regularly recently.

What is the cause of this contam?
can it infect a spore print?
can it be present in a spore syringe?

thank you

jar on left

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Suntzu
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Registered: 10/14/99
Posts: 1,396
Last seen: 1 year, 3 months

Re: Trichoderma (or Penicilium / Aspergi) why?

[Re: aenima]
#586303 - 03/22/02 11:39 AM (21 years, 6 months ago)

Those pics are definitely Asperg./Pen. The source is likely the grain, though could be external. If you're using a tightly screwed on lid w/filter disk or polyfill, odds are it's coming from the grain. If using the metal lids in a not-quite-so clean environment [which could include too-quick of a cooling step] you could be sucking it in from the outside.
If it were a chronic problem, I'd definitely try holding back two negative controls---
1. Jar that is not opened at all, but shaken and incubated with the rest.
2. Jar that is inoculated with a fake injection/inoculation and then shaken and incubated with the rest.

That should narrow the source down pretty well, and you can always use the negative controls later if they're OK.

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aenima
member

Registered: 06/06/01
Posts: 194
Loc: UK
Last seen: 21 years, 2 months

Re: Trichoderma (or Penicilium / Aspergi) why?

[Re: Suntzu]
#587901 - 03/24/02 07:38 AM (21 years, 6 months ago)

i use BRF and verm, and sometimes works, sometimes get the above contam, so i assume it isn't the grain as it would give 100% contam, which it doesn't.

don't use filters, just a 'clea' verm layer, and tape the holes. (am getting some filters tho when get some money)

Do have metal lids, and is a not-so-clean enviroment, and cooling step is quite quick.

Hopefully, when get some filters , and will let cool in a clean envorment, won't suffer so much from this contam. I just thought it weird i get this contam regularly and NEVER had another one.

anyways thanks for your reply

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Seuss
Error: divide byzero

Registered: 04/27/01
Posts: 23,480
Loc: Caribbean
Last seen: 20 days, 13 hours

Re: Trichoderma (or Penicilium / Aspergi) why?

[Re: aenima]
#589064 - 03/25/02 03:01 PM (21 years, 6 months ago)

Do you use the oven tek? If so, try making a quick glove box out of an old cardboard box and some plastic wrap. Spray the inside down with lysol before you use it.

The Penicilium / Aspergillus spore load in my kitchen is _huge_.

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation

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