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Offlineaenima
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Registered: 06/06/01
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Trichoderma (or Penicilium / Aspergi) why?
    #586257 - 03/22/02 12:43 PM (14 years, 11 months ago)

been searching for pictures of the same contam i get all the time, and found some, which are included below (not my pictures, i take no credit etc etc).

It seems most think this is the contam Trichoderma , but i think it was anno who said ti is actually the contam Penicilium / Aspergi. Anyway, this is the ONLY contam i ever get, and have been getting regularly recently.

What is the cause of this contam?
can it infect a spore print?
can it be present in a spore syringe?


thank you





jar on left


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OfflineSuntzu
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Re: Trichoderma (or Penicilium / Aspergi) why? [Re: aenima]
    #586303 - 03/22/02 01:39 PM (14 years, 11 months ago)

Those pics are definitely Asperg./Pen. The source is likely the grain, though could be external. If you're using a tightly screwed on lid w/filter disk or polyfill, odds are it's coming from the grain. If using the metal lids in a not-quite-so clean environment [which could include too-quick of a cooling step] you could be sucking it in from the outside.
If it were a chronic problem, I'd definitely try holding back two negative controls---
1. Jar that is not opened at all, but shaken and incubated with the rest.
2. Jar that is inoculated with a fake injection/inoculation and then shaken and incubated with the rest.

That should narrow the source down pretty well, and you can always use the negative controls later if they're OK.


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Offlineaenima
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Registered: 06/06/01
Posts: 194
Loc: UK
Last seen: 14 years, 7 months
Re: Trichoderma (or Penicilium / Aspergi) why? [Re: Suntzu]
    #587901 - 03/24/02 09:38 AM (14 years, 11 months ago)

i use BRF and verm, and sometimes works, sometimes get the above contam, so i assume it isn't the grain as it would give 100% contam, which it doesn't.

don't use filters, just a 'clea' verm layer, and tape the holes. (am getting some filters tho when get some money)

Do have metal lids, and is a not-so-clean enviroment, and cooling step is quite quick.


Hopefully, when get some filters , and will let cool in a clean envorment, won't suffer so much from this contam. I just thought it weird i get this contam regularly and NEVER had another one.

anyways thanks for your reply


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OfflineSeussA
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Registered: 04/27/01
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Re: Trichoderma (or Penicilium / Aspergi) why? [Re: aenima]
    #589064 - 03/25/02 05:01 PM (14 years, 11 months ago)

Do you use the oven tek? If so, try making a quick glove box out of an old cardboard box and some plastic wrap. Spray the inside down with lysol before you use it.

The Penicilium / Aspergillus spore load in my kitchen is _huge_.


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