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shroomsynergy
Fungi Freak


Registered: 01/17/06
Posts: 861
Loc: Andromeda
Last seen: 4 years, 4 months
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I'm looking for a recipe...
#5855591 - 07/13/06 06:43 PM (17 years, 6 months ago) |
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Does anyone know a good recipe for a LC using potato dextrose agar??? my LC's are 200ml/cc how many grams of agar would be appropriate???
thanx in advance for any info.
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Roadkill
Retired Shroomery Mod


Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: I'm looking for a recipe... [Re: shroomsynergy]
#5855756 - 07/13/06 07:28 PM (17 years, 6 months ago) |
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huh?
you are confusing the fuck out of me!~
an LC and agar are 2 different things.
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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Wronguy
Registered: 03/05/05
Posts: 4,450
Last seen: 12 years, 7 months
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Re: I'm looking for a recipe... [Re: shroomsynergy]
#5855855 - 07/13/06 08:03 PM (17 years, 6 months ago) |
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Your liquid culture substrate would be mixed separately and colonized agar added as spawn. I prefer to use 10 grams of dextrose and 10 grams of light malt extract for every 3-4 cups of water. Once you have your LC sterilized, add a small wedge (about 1/4") of colonized agar. Stir or shake your LC to break up the wedge and allow to incubate for 7-10 days with daily agitation.
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fastfred
Old Hand



Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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Re: I'm looking for a recipe... [Re: Wronguy]
#5856525 - 07/13/06 11:43 PM (17 years, 6 months ago) |
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Potato Dextrose Agar (PDA)(FDA M127) ------------------------------------ 200 g Potato infusion [dilute to 1L total, ~4g solids] 20 g Dextrose 20 g Agar 1 L Distilled water (dH2O)
To prepare potato infusion, boil 200 g scrubbed, sliced(unpeeled) potatoes in 1 liter distilled water for 30 min. Filter through cheesecloth, saving effluent, which is potato infusion (or use commercial dehydrated form). Mix in other ingredients and boil to dissolve. [Dilute to obtain 1 L final volume] Autoclave 15 min at 121°C. Dispense 20-25 ml portions into sterile 15 x 100 mm petri dishes. Final pH, 5.6 ± 0.2.
Medium should not be re-melted more than once. Medium powder is available commercially but may require supplementing with extra agar to a final concentration of 20 g/liter. To BBL or Difco dehydrated medium, add 5 g of agar.
The broth is clear to slightly opalescent and yellowish in color. [1][2] [If you wish to pH to 7, add 0.75-1.0g baking soda.]
-FF
P.S. There is a whole media recipe collection in the archive section under agar.
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