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Shop: Left Coast Kratom Kratom Powder For Sale   North Spore Bulk Substrate, Injection Grain Bag

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Offlineshroomsynergy
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I'm looking for a recipe...
    #5855591 - 07/13/06 06:43 PM (17 years, 6 months ago)

Does anyone know a good recipe for a LC using potato dextrose agar??? my LC's are 200ml/cc  how many grams of agar would be appropriate???

thanx in advance for any info. :thumbup: :thumbup: :thumbup:


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InvisibleRoadkillM
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Re: I'm looking for a recipe... [Re: shroomsynergy]
    #5855756 - 07/13/06 07:28 PM (17 years, 6 months ago)

huh?

you are confusing the fuck out of me!~

an LC and agar are 2 different things.


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineWronguy
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Re: I'm looking for a recipe... [Re: shroomsynergy]
    #5855855 - 07/13/06 08:03 PM (17 years, 6 months ago)

Your liquid culture substrate would be mixed separately and colonized agar added as spawn. I prefer to use 10 grams of dextrose and 10 grams of light malt extract for every 3-4 cups of water. Once you have your LC sterilized, add a small wedge (about 1/4") of colonized agar. Stir or shake your LC to break up the wedge and allow to incubate for 7-10 days with daily agitation.


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Invisiblefastfred
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Re: I'm looking for a recipe... [Re: Wronguy]
    #5856525 - 07/13/06 11:43 PM (17 years, 6 months ago)

Potato Dextrose Agar (PDA)(FDA M127)
------------------------------------
200 g Potato infusion [dilute to 1L total, ~4g solids]
20 g Dextrose
20 g Agar
1 L Distilled water (dH2O)

To prepare potato infusion, boil 200 g scrubbed, sliced(unpeeled) potatoes in 1 liter distilled water for 30 min. Filter through cheesecloth, saving effluent, which is potato infusion (or use commercial dehydrated form). Mix in other ingredients and boil to dissolve. [Dilute to obtain 1 L final volume] Autoclave 15 min at 121°C. Dispense 20-25 ml portions into sterile 15 x 100 mm petri dishes. Final pH, 5.6 ± 0.2.

Medium should not be re-melted more than once. Medium powder is available commercially but may require supplementing with extra agar to a final concentration of 20 g/liter. To BBL or Difco dehydrated medium, add 5 g of agar.

The broth is clear to slightly opalescent and yellowish in color. [1][2]
[If you wish to pH to 7, add 0.75-1.0g baking soda.]


-FF

P.S. There is a whole media recipe collection in the archive section under agar.


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