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0003
squirtforfunandprofit


Registered: 03/28/06
Posts: 104
Loc: new orleans
Last seen: 13 years, 4 months
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slow g2g growth
#5855180 - 07/13/06 04:10 PM (17 years, 6 months ago) |
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This is my first g2g. spores were used to inoculate millet in quart jars. These jars sat since 4-2 unrefrigerated. How much aging could have occurred because of this? Should I spawn bulk substrate with 2nd generation to avoid senescence? Of these jars I have thus far used 3 to spawn 10 jars each of millet.the first batch done on 7-8 is almost complete, while the second 2 batches done on 7-11 are showing much slower signs of recovery. Some jars show almost no growth. The strain for these 3 is Koh Samui. The only difference in substrate is that the first batch had more bursted seeds and might have been wetter. I believe there is sufficient moisture content in the millet. Why would the first batch grow faster if its the same strain?
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0003
squirtforfunandprofit


Registered: 03/28/06
Posts: 104
Loc: new orleans
Last seen: 13 years, 4 months
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Re: slow g2g growth [Re: 0003]
#5855576 - 07/13/06 06:39 PM (17 years, 6 months ago) |
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bump
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0003
squirtforfunandprofit


Registered: 03/28/06
Posts: 104
Loc: new orleans
Last seen: 13 years, 4 months
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Re: slow g2g growth [Re: 0003]
#5855749 - 07/13/06 07:25 PM (17 years, 6 months ago) |
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I'm afraid these jars won't make it so I want to avoid this problem with the rest of my g2g's I have to do. Please help.
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liamtheloser
Advanced Idiot

Registered: 06/07/06
Posts: 1,453
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Re: slow g2g growth [Re: 0003]
#5855793 - 07/13/06 07:40 PM (17 years, 6 months ago) |
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senesence(sp) wont happen through cloning or g2g for quite a while I believe... something like hundreds of clones/g2g transfers... I wouldn't worry about that.
And the first batch may grow faster because of moisture content (like you stated) or the grains from your g2g didnt have super strong genetics on your 2nd batch.. Could be a host of reasons, but I know that the whole senesence(sp) thing doesn't happen for a while, and it usually only happens when you clone, and print and print and print and print and print andthen clone and print, etc etc for 100 times or so.
I'm probably wrong, since I'm not too 'into' the whole genetics thing, but from what i've read, it takes quite a while for your strain to run dry.
It's probably just an issue with your substrate.
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fastfred
Old Hand



Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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Senesence only occurs from repeated subculturing. Not from using prints or spores.
Allowing the strain to complete the sexual cycle by using it's spores is what reinvigorates it and prevents eventual senesence.
-FF
P.S. pwnd you
-------------------- It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy The difference between the substances doesn't matter. This is a war on consciousness, on our right to the very essence of what we are. With no control over that, we have no need to speak of freedom or a free society. -fireseed "If we are going to have a war on marijuana, the least we can do is pull the sick and the dying off the battlefield." -Neal Levine (MPP) I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid
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liamtheloser
Advanced Idiot

Registered: 06/07/06
Posts: 1,453
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Re: slow g2g growth [Re: fastfred] 1
#5856561 - 07/13/06 11:58 PM (17 years, 6 months ago) |
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damn you fred, always pwning me!
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