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Offlinedavesj1
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Registered: 04/11/05
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Shake immediately after inoculation or after some growth?
    #5852683 - 07/12/06 09:46 PM (17 years, 6 months ago)

I am planning on inoculating a large filter patch bag OF WBS w/ about 40 CC of LC. Should I massage the bag immediately after inoculation to spread the liquid around or should I wait for some growth first? If i should wait, how much growth should wait for before massaging?

Or, should i do both?


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OfflineRogerRabbitM
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Re: Shake immediately after inoculation or after some growth? [Re: davesj1]
    #5852700 - 07/12/06 09:49 PM (17 years, 6 months ago)

With LC, go ahead and knead it right away. With spores, I like to let them germinate in close proximity to each other so they can match up, then knead or shake.
RR


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Offlinesplifner180
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Re: Shake immediately after inoculation or after some growth? [Re: RogerRabbit]
    #5852763 - 07/12/06 09:59 PM (17 years, 6 months ago)

With LC I like to mix at inoculation and again around 30%, give or take.

splif


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Offlinedavesj1
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Registered: 04/11/05
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Re: Shake immediately after inoculation or after some growth? [Re: splifner180]
    #5852799 - 07/12/06 10:06 PM (17 years, 6 months ago)

Thanks guys, u too are always a big help. Im gonna shake after inoculation and then if i need to do it again later I will.


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Offlinesplifner180
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Re: Shake immediately after inoculation or after some growth? [Re: davesj1]
    #5855682 - 07/13/06 07:07 PM (17 years, 6 months ago)

Just keep in mind that shaking causes the myc to seperate. It takes 24 hours to re-knit and begin to grow again so don't over-do it. If you shake up the jars too many times they'll stall for lack of moisture.

splif


--------------------
First Grow: Ecuador -> LC -> HPoo/Straw -> Monotub
Build a Do-It-Yourself Magnetic Stirrer in thirty minutes with no money.


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Invisibleliamtheloser
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Registered: 06/07/06
Posts: 1,453
Re: Shake immediately after inoculation or after some growth? [Re: splifner180]
    #5855811 - 07/13/06 07:45 PM (17 years, 6 months ago)

24 hours is kind of general :P

But yeah, I shake after inoculation with an LC, then at ~30 percent.
After the 30 percent shake, it usually recovers between 12-36 hours depending on the strain (or substrain).


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