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Offline2FiNiTe
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Registered: 06/12/06
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Creating a LC without spores? Possible or Not
    #5850730 - 07/12/06 12:48 PM (17 years, 6 months ago)

I've got the Tek I plan on following to create my first LC. I'm wondering if theres alternate ways to create one other than using spores. I've heard things like "mushroom slurrys" "Inside base of a stem" all kinds of things like this. I've got mexi-cubes fruiting right now and I've saved a few to get a spore print from and I'm going to use those spores to create another LC but the shroomys arent sporolating yet. So basically I'm wondering how else can I do it?


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"Ours is a world of nuclear giants and ethical infants. We know more about war that we know about peace, more about killing that we know about living."

General Omar N. Bradley


Edited by 2FiNiTe (07/12/06 01:32 PM)


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Offlineroyer
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Re: Got an LC Tek, Now what? [Re: 2FiNiTe]
    #5850780 - 07/12/06 01:07 PM (17 years, 6 months ago)

saved a few to get spore prints from? you got to get it right after you pick. you cant pick lrt it dry then take a print. you could make a slurry.


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Offline2FiNiTe
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Re: Got an LC Tek, Now what? [Re: royer]
    #5850785 - 07/12/06 01:09 PM (17 years, 6 months ago)

No what I meant is that I left a few Mexi-Cubes growing, waiting on sporolation. I havested the rest.


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"Ours is a world of nuclear giants and ethical infants. We know more about war that we know about peace, more about killing that we know about living."

General Omar N. Bradley


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InvisibleJoeMama
Registered: 04/28/06
Posts: 154
Re: Got an LC Tek, Now what? [Re: 2FiNiTe]
    #5851306 - 07/12/06 04:18 PM (17 years, 6 months ago)

What you might want to try (I plan to try this) is take one of your freshly havested shroomies, and (in the glovebox of course) cut off the bottom of the stem with flamed scisors. Break open the stem and pull out some of the meat within the stem (with flamed tweezers) dip this meat in a 3% Hydrogen Peroxide solution (right out of the bottle) then transfer this to your LC jar. If you dont want to use the inside of the stem you can use the underside of the fresh cap (minus the gills/spores) Just be very clean and do this within a glovebox. Good luck!
-Me


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Offline2FiNiTe
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Re: Creating a LC without spores? Possible or Not [Re: 2FiNiTe]
    #5852234 - 07/12/06 08:25 PM (17 years, 6 months ago)

I know there has to be other ways, someone anyone pleaseeee help


--------------------
"Ours is a world of nuclear giants and ethical infants. We know more about war that we know about peace, more about killing that we know about living."

General Omar N. Bradley


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InvisibleJoeMama
Registered: 04/28/06
Posts: 154
Re: Creating a LC without spores? Possible or Not [Re: 2FiNiTe]
    #5852375 - 07/12/06 08:51 PM (17 years, 6 months ago)

I have also read people inserting a needle directly into the stem itself to capture culture in the needle itself. Then pushing the water from the syringe thru the needle (thus pushing the culture out) into your self healing injector port on the LC jar. (I havent tried it but its worth a shot)

Do yourself a favor and expiriment a bit. :smile: What can it hurt?
-Me


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Invisiblecreamcorn
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Re: Creating a LC without spores? Possible or Not [Re: JoeMama]
    #5852398 - 07/12/06 08:56 PM (17 years, 6 months ago)

^^^^ yup, is a great way. cloning without the agar. LC without the spores. simple, sterile, and effective. it has it all! one of these days i gotta make a pictoral tek of it just to refer people to because its really a good method.

the only thing i'd add, is that its a good idea to use an isopropyl or iodine soaked cotton swab to wipe the outside of the stem down first, just as if a doctor were to wipe your arm before giving you a shot. don't want to bring any contams that may be on the surface along with you to your LC. have also had more luck using a sample from the base of the stem, closer to the growing surface/farther from the cap.


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