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Toxxy
Random Gary
Registered: 07/07/06
Posts: 36
Last seen: 16 years, 10 months
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Reusing syringes / Mixing spores & LC
#5848658 - 07/11/06 09:07 PM (17 years, 6 months ago) |
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I'll be getting a syringe (S. American) from Spores101 in a couple days. It will mark my first venture into the world of mycology. It was previously advised that I begin my first grow with LC. I don't have syringes lying around, so I was thinking: Maybe I can use the spore syringe for my LC.
Here's my idea: Knock up a jar of honey/water by following Otto's beginner-friendly LC tek, using the syringe from Spores101.
Wait a week or so for LC to mature.
Begin innoculating BRF jars with leftover 8cc's from syringe.
After depleting syringe, re-inoculate BRF jars with LC. That way, not only do I have some fresh spores ready to go to work, but I'll also have some mycelium colonizing the jars.
Has anyone tried this? What if I inoculated my BRF jars at the same time as the LC, then re-inoculated with LC after the BRF jars started to colonize?
Are spores101 syringes even re-usable, or is this just a really stupid idea?
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liamtheloser
Advanced Idiot

Registered: 06/07/06
Posts: 1,453
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Re: Reusing syringes / Mixing spores & LC [Re: Toxxy]
#5848720 - 07/11/06 09:16 PM (17 years, 6 months ago) |
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what i do is make an lc, then multi-spore some pf cakes, then when my syringe is empty, i'll sterlize some karo-water and suck it into the empty syringe... the spores that clung to the walls of the syringe actually will make a little LC inside of the syringe itself :P it's like having a little extra LC around JIC.
But I'd invest in some 14-16 gauge blanks for LC's, because usually the spore syringes from spores101 are smaller... 18-20
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creamcorn
mad scientist


Registered: 03/13/06
Posts: 2,962
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Re: Reusing syringes / Mixing spores & LC [Re: Toxxy]
#5848724 - 07/11/06 09:17 PM (17 years, 6 months ago) |
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sure you can reuse syringes. rinse out, wrap in foil and boil for 30 mins or PC for 15 minutes.
your plan sounds great. except... you don't want to re-inoculate after they start to colonize. just do your LC and first set of jars at the same time until the syringe is gone. let the LC colonize and save that LC for the next round of jars (or start more once its ready) and you can keep going for quite a while without worrying about any more spores... that way you can get comfortable and have enough "starter culture" on hand to get you going before you have to worry about making prints or preparing your own syringes for a little while.
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etam
it's a hobby...


Registered: 04/24/06
Posts: 1,126
Loc: East Coast
Last seen: 15 years, 3 months
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Re: Reusing syringes / Mixing spores & LC [Re: creamcorn]
#5849236 - 07/11/06 11:14 PM (17 years, 6 months ago) |
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On another note, I've actually received a Hawaiian syringe from Spores101 in which some LC had already begun colonizing the syringe when I received it.
It kind of spooked me at first, but everything went alright.
Sucking some sterile karo water into the syringe after it's spent is a great idea. I believe I'll start doing that with my own spent syringes.
Also, as advised, innoculating once is enough.
Edited by etam (07/11/06 11:50 PM)
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