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InvisibleBlutjager
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Someone please help me !!!!
    #5848106 - 07/11/06 06:43 PM (17 years, 6 months ago)

After 4 attempts(and 29 contaminated jars)I have decided that wild bird seed is just not for me.I understand why some of them went bad because of bacteria,probably due to not soaking enough,but even the ones that didn't still got the green :confused:.I am thinking maybe cracked corn next or pop corn next time since I ran out  of wbs anyway.I used the search and still only found a few things on each.Can someone link me to a few good teks? What I did find didn't mention having to soak the corn.Does it not harbor endospores for bacteria?Also I couldn't be more clean when I work.Mask,gloves,Lysol,shower cap,everything sprayed with Lysol,Flaming and alcohol wiping after every jar.Is it possible that my house is just poisoned(Maybe due to the giant green clouds of green that come out when I dump bad jars)I will not give up,I enjoy this hobby too much and plus I invested to much in it already.Such as my $180 PC and $120 worth of poo just waiting for something to spawn into.PLEASE SOMEONE HELP ME


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InvisibleAtheist
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Re: Someone please help me !!!! [Re: Blutjager]
    #5848126 - 07/11/06 06:46 PM (17 years, 6 months ago)

either your not pressure cooking long enough

or you have dirty air, which you probably do with the green cloud thing you said

buy some Oust and spray a shitload of it all over the area before you inoculate

dont soak, do the simmer tek by foomanshroom


Edited by Atheist (07/11/06 08:41 PM)


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InvisibleBlutjager
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Re: Someone please help me !!!! [Re: Atheist]
    #5848215 - 07/11/06 07:02 PM (17 years, 6 months ago)

Oddly enough that is the tek I used every time,It seems the easiest.But its not working for me.Either green or bacteria every single jar.The incubators is now empty and its time to try again.As I said I think I'm giving up on wbs.Any advice anyone please?


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Invisiblefastfred
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Re: Someone please help me !!!! [Re: Blutjager]
    #5848220 - 07/11/06 07:04 PM (17 years, 6 months ago)

> After 4 attempts(and 29 contaminated jars)I have decided that wild bird seed is just not for me.

Yep. WBS sucks. Use rye grain. Rye is by far the best substrate to grow on IMHO.

> I couldn't be more clean when I work.Mask,gloves,Lysol,shower cap,everything sprayed with Lysol,Flaming and alcohol wiping after every jar.

Yep if you had a flow hood it would just push the contamination back to the casing stage. (assuming you're not spawning with it) If you did the casing with sterilized casing in a flow hood it would just push the contamination back to the fruiting stage! I learned this the hard way. WBS just plain sucks! I feel sorry for all the people who try and fail at it because people here push it like it's a good way to go.

> Is it possible that my house is just poisoned?

Nope, it's the WBS. It's too sweet, contams love it. Maybe it's something about location, but you can have super-duper cleanliness and sterility, AND be able to do rye with 100% success but completely and repeatedly fail at WBS no matter how carefull you are or how many times you try.

> PLEASE SOMEONE HELP ME

Start with a PF tek grow, if you haven't already. Then move up to rye grain. Rye works great.


-FF


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InvisibleBlutjager
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Re: Someone please help me !!!! [Re: fastfred]
    #5848308 - 07/11/06 07:24 PM (17 years, 6 months ago)

Thank you Fastfred,I did do a pf tek grow and it also contaminated on me,I want grain to use as spawn so I can use my hpoo.I bought so much hpoo and I would hate for me not to get to use it,But nothing will colonize successfully for me.Either way I'm done with wbs,So now the quest for rye begins,I looked a few places and haven't found it yet but I will.5 shrooms for you


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OfflineRogerRabbitM
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Re: Someone please help me !!!! [Re: Blutjager]
    #5848360 - 07/11/06 07:37 PM (17 years, 6 months ago)

When you have contamination on everything, you must look beyond your substrate. Read up on sterile procedures and I'm sure you can find where you're going wrong. I agree that rye grain is a superior spawn material, but I don't think that is your problem.
RR


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Invisiblefastfred
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Re: Someone please help me !!!! [Re: RogerRabbit]
    #5848497 - 07/11/06 08:17 PM (17 years, 6 months ago)

> I did do a pf tek grow and it also contaminated on me.

There must be a flaw in your sterile technique. Have no fear, you'll master it eventually. I know it's tough to improve when you're having no success. A lot of it is knowing the ins and outs of sterile technique and moving quickly and smoothly.

Google "sterile technique" and "aseptic technique". Read all you can on it. Microbiology lab manuals are very helpful. Avoid all drafts. A bunsen burner is very helpful. Gloveboxes are also good to have. A flow hood works wonders, but you still need good sterile technique.

Working with agar is a very good way to learn sterile technique. Do some petri's, slants, and mini LCs in test tubes. You can practice with interesting molds or bacteria you can culture from the air.

Environment is a big factor. Running a hepa in the room you use for a few hours could help a lot. Also try using a tall desk or counter. 90%+ of dust and contams are within 1 foot of the floor.


-FF


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Invisibleliamtheloser
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Re: Someone please help me !!!! [Re: fastfred]
    #5848547 - 07/11/06 08:32 PM (17 years, 6 months ago)

I just did 20 jars of wbs and 4 of lc, only one of them contaminated... and i've done some crazy shit with these jars too. open air innoc's, putting a dirty towel in the incubation chamber with them, etc etc etc... I think it's either your home or your sterile technique that's causing you problems. Either that or the WBS you use has a fungicide coating... which would explain the bacteria taking hold before your spores could germ... anyways, go with rye, never used it but if fastfred and rogerrabbit say it's good... DO IT!


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InvisibleBlutjager
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Re: Someone please help me !!!! [Re: liamtheloser]
    #5848769 - 07/11/06 09:24 PM (17 years, 6 months ago)

I did just make a alcohol lamp.And I just may get an air filter,I do know that it must be my fault.I just wish that I could figure out where I'm going wrong.I will go and look for rye tomorrow,and I already have 2 bags of cracked corn.I'm just going to keep trying no matter how many times I goof up.I know its not me so it must be my environment and I clean that too.Perhaps a glove box may be all I need.I guess the only way to find out is to make one.


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Invisiblecreamcorn
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Re: Someone please help me !!!! [Re: Blutjager]
    #5848838 - 07/11/06 09:38 PM (17 years, 6 months ago)

a useful troubleshooting method that nobody has mentioned yet is make a control jar. go ahead and prep a jar or two like you've been doing. incubate it and everything. just skip the inoculation - no spores.

this will help you narrow it down. if they don't contam, you know the problem is either with your inoculation technique (likely) or with the spores (possible but not as likely). if it contams anyway, then your problem is with how you prepare the jars in the first place.

its useful even if you're switching to a new substrate... make one extra jar that you don't inoculate, just for comparison sake down the line should something go wrong again.

at least you'll have an idea of where the problem is.


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InvisibleBlutjager
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Re: Someone please help me !!!! [Re: creamcorn]
    #5848948 - 07/11/06 10:10 PM (17 years, 6 months ago)

Thanks again creamcorn,I will do this for sure on the next batch for sure.Regardless of what substrate or changes to my tek I can come up with for improvement.I appreciate all the help from all of you who know what your doing,and I look forward do giving back to this community when I know what I'm doing and I can help people with questions that I know the answers to


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