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OfflinedeXtrous
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Registered: 04/24/06
Posts: 1,743
Loc: Australia
Last seen: 9 months, 27 days
I hope I don't get contams- what do you think?
    #5844660 - 07/10/06 10:05 PM (17 years, 6 months ago)

Hey, I just put 9 PF TEK jars into my incubater.

The reason I am making this thread is that because when I pc'd my jars the lids might've loosened a fraction and let some moisture into the jars, and I know contams like moisture :frown:

I inoculated this morning out of my glovebox because I couldn't get the right angles to insert my syringe into the cakes (due to a small glovebox). I still flame sterilised and used isop. 70% alcohol to sterilise the area.

Mabye some reassurance or am I fucked? I'm pretty sure I hit everything else spot on.


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Invisibledecepticon
someone abovethe crowd

Registered: 07/10/06
Posts: 113
Loc: europa
Re: I hope I don't get contams- what do you think? [Re: deXtrous]
    #5844746 - 07/10/06 10:20 PM (17 years, 6 months ago)

when you see green,
you are screwed.

until then just look for the white and keep your hopes up.
start a new project.
be patient.

report back with pictures if you expect a contam


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OfflinedeXtrous
complete tool
Male

Registered: 04/24/06
Posts: 1,743
Loc: Australia
Last seen: 9 months, 27 days
Re: I hope I don't get contams- what do you think? [Re: decepticon]
    #5844822 - 07/10/06 10:32 PM (17 years, 6 months ago)

Surely will post some pics up here.

myc starts growing after about 5 days in the right conditions right?


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Invisiblemonstermitch
Growing in Bags Doesn't Work

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Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay Flag
Re: I hope I don't get contams- what do you think? [Re: deXtrous]
    #5846568 - 07/11/06 11:31 AM (17 years, 6 months ago)

no, not always.

sometimes it could take a month.
especially if spores have to germinate first.


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Offlineismokeweed
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Registered: 11/19/05
Posts: 286
Last seen: 14 years, 9 months
Re: I hope I don't get contams- what do you think? [Re: monstermitch]
    #5847742 - 07/11/06 05:10 PM (17 years, 6 months ago)

i don't know about that one. i've always gotten the first signs of mycelium growth within a week. the first little white spots. and im pretty sure the time before that is the time it takes to germinate. im no expert, thats just what i've seen.


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Invisibledecepticon
someone abovethe crowd

Registered: 07/10/06
Posts: 113
Loc: europa
Re: I hope I don't get contams- what do you think? [Re: ismokeweed]
    #5848510 - 07/11/06 08:20 PM (17 years, 6 months ago)

Quote:

ismokeweed said:
i don't know about that one. i've always gotten the first signs of mycelium growth within a week. the first little white spots. and im pretty sure the time before that is the time it takes to germinate. im no expert, thats just what i've seen.




lucky you.
don't rule out the possibility though.
I agree, I have seen it take a month.
not often, but it happens.


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OfflinedeXtrous
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Male

Registered: 04/24/06
Posts: 1,743
Loc: Australia
Last seen: 9 months, 27 days
Re: I hope I don't get contams- what do you think? [Re: decepticon]
    #5849617 - 07/12/06 01:47 AM (17 years, 6 months ago)

A month under worse conditions probably.

I am using a controlled heat in an incubater which should (in theroy) speed things up.


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Offlineroyer
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Registered: 05/15/06
Posts: 4,801
Loc: anywhere but here
Last seen: 4 years, 10 months
Re: I hope I don't get contams- what do you think? [Re: deXtrous]
    #5849917 - 07/12/06 05:53 AM (17 years, 6 months ago)

i have done lots of jars like that before and they worked just fine..


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if you have any questions please feel free to pm me , thx :-)


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OfflineRemainRandom50
Do You Need ToKnow Me?
Registered: 01/15/06
Posts: 1,695
Last seen: 14 years, 9 months
Re: I hope I don't get contams- what do you think? [Re: royer]
    #5854927 - 07/13/06 02:42 PM (17 years, 6 months ago)

it should work fine, just watch out for trichy or any other contam.


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At times I get consumed by my everyday life and will leave the Shroomery. Yet, every time drugs come falling into my life for fun.....I always think about the Shroomery and then I'm back!


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