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Synaethesia
Astral BodySalesman


Registered: 06/07/05
Posts: 60
Loc: Flatland
Last seen: 17 years, 2 months
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Shroomery, why am I inept at Liquid Culture?
#5843473 - 07/10/06 05:37 PM (17 years, 6 months ago) |
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Well not that I expect you to really know already, but I've tried my luck with Liquid culture 4 times now with well over 20 jars and have only had two work. I've used the light dry malt/dry dex method and each time they come out with a ton of sediment and really watery (not sure if this matters). I then inject 1-2cc of spores and let them be (shake a few times sometimes) and nothing happens. I pc for around 30 min at 15 psi and have a single tube coming out the top for FAE.
Do these need to be incubated in complete darkness or something, and since it's summer I've left them out of the incubator, but would it be better to keep them warm?
Finally, if you could give me a tried and tested malt/dex recipe that would be ace. My fun has come to a complete stop because I can't get any mycelium culture going and I really don't want to go back to agar right now.
-------------------- Load Universe into cannon. Aim at brain. Fire. ~Nailing Bee to the Honey
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EquilibriuM
dream stalker

Registered: 07/17/05
Posts: 2,323
Last seen: 16 years, 7 months
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Re: Shroomery, why am I inept at Liquid Culture? [Re: Synaethesia]
#5843502 - 07/10/06 05:43 PM (17 years, 6 months ago) |
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1 table spoon each per 2 cups water.
sediment doesn't matter. mycelium will grab onto it and colonize from there.
30 minutes is long. Go for 15.
-------------------- HELP!!!!!!!!!
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fastfred
Old Hand



Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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Re: Shroomery, why am I inept at Liquid Culture? [Re: EquilibriuM]
#5843550 - 07/10/06 05:53 PM (17 years, 6 months ago) |
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Make sure you're using unhopped malt. Extra light unhopped spray malt extract (powder) is what you want. Use 2-4% malt. 30g/L is the standard mix. That's 3g/100mL.
For karo use 5ml/100ml. That's 5cc/100ml or 50ml/L.
It's best to keep them in the dark. You should have no problems getting spores to germinate. I recommend 20 min sterilization.
-FF
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Tippinthru
contented

Registered: 04/07/05
Posts: 1,131
Loc: "The Garden"...
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Re: Shroomery, why am I inept at Liquid Culture? [Re: Synaethesia]
#5843565 - 07/10/06 05:58 PM (17 years, 6 months ago) |
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1 (one) tablespoon each (leveled off with a butter knife) of light dry malt & dry dextrose (or Karo syrup) to 1 (one) quart of boiling (rolling boil) filtered or bottled water & stir well.
While still HOT, pour through 4 or 5 stacked coffee filters into 2 (two) quart jars (1/2 each). Which leaves ample space for air/gas/oxygen in the jar.
Add lids, with gas exchange provision. Add a marble or 2, if you wish, as an agitator device.
With lids 1/4 to 1/2 turn loose, PC @ 15 psi FOR 20 (TWENTY) minutes.
Remove jars while still warm & tighten lids.
Cool 12 hours, or overnight.
Inject 2 (+/-) Ml of VIABLE spore solution & incubate 75 to 80F.
Agitate daily.
  1/2 gallon jar with 1 quart LC solution day 5. (done as above & never failed yet )
Light dry MALT will give it a gold (beer)color.
  This one is dextrose alone.
-------------------- Perfection is attained by slow degrees; it requires the hand of time... [
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Synaethesia
Astral BodySalesman


Registered: 06/07/05
Posts: 60
Loc: Flatland
Last seen: 17 years, 2 months
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Re: Shroomery, why am I inept at Liquid Culture? [Re: Tippinthru]
#5846562 - 07/11/06 11:30 AM (17 years, 6 months ago) |
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Thanks friends. I'll try again today, maybe it will work this time.
-------------------- Load Universe into cannon. Aim at brain. Fire. ~Nailing Bee to the Honey
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Oatman2000
-=Outa Space=-



Registered: 05/10/05
Posts: 2,877
Loc: Planetary Nebula
Last seen: 1 year, 4 months
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Re: Shroomery, why am I inept at Liquid Culture? [Re: Synaethesia]
#5846791 - 07/11/06 12:34 PM (17 years, 6 months ago) |
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best thing i have purchased for this hobby.... Magnetic Stirer.
you can even cheeply make your own... tons of different ways. My LC failed too untill i bought that, now I have about 6 different strains on had at all times...
--------------------
Spawning to COIR
My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION ----------------------------
4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine
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TheBotanist
Stranger
Registered: 03/02/06
Posts: 48
Last seen: 16 years, 5 months
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Re: Shroomery, why am I inept at Liquid Culture? [Re: Oatman2000]
#5846910 - 07/11/06 01:02 PM (17 years, 6 months ago) |
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light karo syrup is so much easier.
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Synaethesia
Astral BodySalesman


Registered: 06/07/05
Posts: 60
Loc: Flatland
Last seen: 17 years, 2 months
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Re: Shroomery, why am I inept at Liquid Culture? [Re: Synaethesia]
#5846919 - 07/11/06 01:06 PM (17 years, 6 months ago) |
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Yeah I"m keeping my out for one, but they're not the cheapest things ever. I hate this hobby becauses it forces me into buying tons of cool scientific equipment that is hard to explain to people why you have it.
-------------------- Load Universe into cannon. Aim at brain. Fire. ~Nailing Bee to the Honey
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