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OfflineMurit
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30 psi 270f Flashing suitable for substrate?
    #5842420 - 07/10/06 01:17 PM (17 years, 6 months ago)

okay...so after my very uneducated post last night, I delved deeper into sterilization methods and discovered a method called 'flashing' which involves using an autoclave to sterilize the jars at 30psi (equalling out to 270deg F) and many claims that this method indeed will sterilize its contents in aproximately 7-8 minutes (given a few minutes for the substrate to heat to the aforementioned temp)
would this be appropriate for the substrate? or would a higher temp damage it in some way?

Ah...nevermind. Found info about it in an old medical journal...it wouldn't work. Appearently 'flashing' is only for small instruments (such as needles/scalples or otherwise)


--------------------
"Halucinogens produce severe
and violent reactions..
..in those who have never tried them."
- Terence McKenna


Edited by Murit (07/10/06 01:23 PM)


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OfflineEquilibriuM
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Re: 30 psi 270f Flashing suitable for substrate? [Re: Murit]
    #5842464 - 07/10/06 01:29 PM (17 years, 6 months ago)

how would you get it to 30psi anyway?


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OfflineMurit
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Re: 30 psi 270f Flashing suitable for substrate? [Re: Murit]
    #5842486 - 07/10/06 01:33 PM (17 years, 6 months ago)

autoclaves are designed to reach higher PSI than PCs
here's some info on em.
http://caltest.vet.upenn.edu/surgery/2220.htm


--------------------
"Halucinogens produce severe
and violent reactions..
..in those who have never tried them."
- Terence McKenna


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Offlinetonyperez420
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Re: 30 psi 270f Flashing suitable for substrate? [Re: Murit]
    #5842547 - 07/10/06 01:51 PM (17 years, 6 months ago)

would any of those work for mycology purposes?

It looks like a nice piece of equip to add to the collection

:discodance:


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InvisibleAtheist
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Re: 30 psi 270f Flashing suitable for substrate? [Re: tonyperez420]
    #5842554 - 07/10/06 01:54 PM (17 years, 6 months ago)

you should delete that other thread with the same name


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Invisibleesdfsfd
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Re: 30 psi 270f Flashing suitable for substrate? [Re: Atheist]
    #5842588 - 07/10/06 02:04 PM (17 years, 6 months ago)

why not just autoclave for 1 hour at 15 p.s.i.? :evildog: :eaglekitty: :freakybirdthing:


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Offlinetonyperez420
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Re: 30 psi 270f Flashing suitable for substrate? [Re: esdfsfd]
    #5842703 - 07/10/06 02:29 PM (17 years, 6 months ago)

Quote:

esdfsfd said:
why not just autoclave for 1 hour at 15 p.s.i.? :evildog: :eaglekitty: :freakybirdthing:




that sounds really caliente for some substrate, for 1 hour?!?!


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OfflineEquilibriuM
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Re: 30 psi 270f Flashing suitable for substrate? [Re: tonyperez420]
    #5842714 - 07/10/06 02:32 PM (17 years, 6 months ago)

Quote:

tonyperez420 said:
Quote:

esdfsfd said:
why not just autoclave for 1 hour at 15 p.s.i.? :evildog: :eaglekitty: :freakybirdthing:




that sounds really caliente for some substrate, for 1 hour?!?!




I do mine for 3-4.


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Offlinetonyperez420
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Re: 30 psi 270f Flashing suitable for substrate? [Re: EquilibriuM]
    #5842738 - 07/10/06 02:39 PM (17 years, 6 months ago)

Quote:

EquilibriuM said:
Quote:

tonyperez420 said:
Quote:

esdfsfd said:
why not just autoclave for 1 hour at 15 p.s.i.? :evildog: :eaglekitty: :freakybirdthing:




that sounds really caliente for some substrate, for 1 hour?!?!




I do mine for 3-4.




sorry I am stoned, its that time of the day for me

but for poo/straw that sounds hot is probably what I meant to say

must be the outside heat then, its burning!!!
:bouncysmoke:


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Invisiblecreamcorn
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Re: 30 psi 270f Flashing suitable for substrate? [Re: EquilibriuM]
    #5842749 - 07/10/06 02:43 PM (17 years, 6 months ago)

sure, you could "flash sterilize" substrate, 7-8 minutes at 270F should work out (contams die much quicker as heat increases), but the entire contents of your jar/bag/whathaveyou needs to be at 270F, and its still going to take a half hour+ to get there. flash sterilizing is normally done on medical instruments, things where the surface needs to be sterilized, you don't have a large mass of moist "stuff" that needs to get up to that temp, which takes some time.

if you still want to shave time off your sterilizing (because i know waiting an hour in a month plus long process is just the worst thing ever) go check and see what a medical autoclave capable of 30psi costs as well as the logistics of actually owning/installing and running one in your home and you'll understand why hobbyists use pressure cookers.


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OfflineRogerRabbitM
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Re: 30 psi 270f Flashing suitable for substrate? [Re: creamcorn]
    #5842900 - 07/10/06 03:27 PM (17 years, 6 months ago)

Grain spawn should be sterilized. Substrates should be pasteurized.
RR


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OfflineEquilibriuM
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Re: 30 psi 270f Flashing suitable for substrate? [Re: RogerRabbit]
    #5842916 - 07/10/06 03:30 PM (17 years, 6 months ago)

Quote:

RogerRabbit said:
Grain spawn should be sterilized. Substrates should be pasteurized.
RR




Unless you skip the spawn step and inject your sterile substrate with LC.


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OfflineRogerRabbitM
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Re: 30 psi 270f Flashing suitable for substrate? [Re: EquilibriuM]
    #5842922 - 07/10/06 03:32 PM (17 years, 6 months ago)

Try injecting an LC to sterilized straw or manure and see what happens.
RR


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Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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OfflineEquilibriuM
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Re: 30 psi 270f Flashing suitable for substrate? [Re: RogerRabbit]
    #5842942 - 07/10/06 03:38 PM (17 years, 6 months ago)

I do it all the time. Works great. Of course, I have Grain and chunky vermiculite in there as well...


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Offlinetonyperez420
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Re: 30 psi 270f Flashing suitable for substrate? [Re: creamcorn]
    #5842972 - 07/10/06 03:44 PM (17 years, 6 months ago)

Quote:

creamcorn said:
flash sterilizing is normally done on medical instruments, things where the surface needs to be sterilized, you don't have a large mass of moist "stuff" that needs to get up to that temp, which takes some time.




are you talking about a scalpel, surgery tools, etc... this may sound stupid but why not use rubbing alcohol... or some kind of chemical?!?!


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OfflineEquilibriuM
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Re: 30 psi 270f Flashing suitable for substrate? [Re: tonyperez420]
    #5842980 - 07/10/06 03:46 PM (17 years, 6 months ago)

thats not sterilizing. thats sanitizing.


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Offlinetonyperez420
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Re: 30 psi 270f Flashing suitable for substrate? [Re: RogerRabbit]
    #5842994 - 07/10/06 03:48 PM (17 years, 6 months ago)

Quote:

RogerRabbit said:
Grain spawn should be sterilized. Substrates should be pasteurized.
RR




thanks for correcting me

I got lots to learn... I KNOW

btw like your site... http://www.mushroomvideos.com


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OfflineMurit
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Re: 30 psi 270f Flashing suitable for substrate? [Re: tonyperez420]
    #5843159 - 07/10/06 04:16 PM (17 years, 6 months ago)

yes...I realized that flashing was only really useful for tools just shortly after I made the post...but figured I'd leave the thread live just so other noobs like me would learn from it, possibly without having to ask...though...I rather doubt it.

Also, just to clarify, I meant spawn...not substrate, thanks for pointing out that error, RR.

Anyhow...I've just become fortunate enough to come by a small autoclave, and have been looking for a means of utilizing it to a more efficient method than taking such a lengthy process of sterilization....but then again, being such a noob at this, I suppose I have yet to truely learn the greatest lesson this hobby can teach any of us (without consuming the crops, anyhow)...patience.

Again, everyone...thank you for your input. And, as always, it is valued and respected.


--------------------
"Halucinogens produce severe
and violent reactions..
..in those who have never tried them."
- Terence McKenna


Edited by Murit (07/10/06 04:40 PM)


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