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OfflineFungi_x
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To Clone or Not to Clone, Thats TeH Question
    #5842392 - 07/10/06 03:09 PM (14 years, 9 months ago)

You think its worth the extra work? Also I'm searching through the FAQ's and shit not finding very much on the subject, can anyone hook it up with a few links if you think you have some links that will give me a good idea on cloning.


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InvisibleTippinthru
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Re: To Clone or Not to Clone, Thats TeH Question [Re: Fungi_x]
    #5842440 - 07/10/06 03:22 PM (14 years, 9 months ago)

Best simple method I have found is to use a large bore (14g) needle.
(on a sterile syringe)
And, take a core sample.
(PREFERABLY - working in a glove box)

First cut stem about 2 inch in length.
Drop in hydrogen peroxide for a few minutes.
Remove stem, insert needle into stem.
While needle is inserted, bend stem, rotate needle 360 degrees.
Withdraw needle.
Needle should have a core sample in it.

Inject core sample into a prepared LC jar. :grin:


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Perfection is attained by slow degrees; it requires the hand of time...
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OfflineEquilibriuM
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Registered: 07/17/05
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Re: To Clone or Not to Clone, Thats TeH Question [Re: Tippinthru]
    #5842460 - 07/10/06 03:27 PM (14 years, 9 months ago)

Quote:

Tippinthru said:
Best simple method I have found is to use a large bore (14g) needle.
(on a sterile syringe)
And, take a core sample.
(PREFERABLY - working in a glove box)

First cut stem about 2 inch in length.
Drop in hydrogen peroxide for a few minutes.
Remove stem, insert needle into stem.
While needle is inserted, bend stem, rotate needle 360 degrees.
Withdraw needle.
Needle should have a core sample in it.

Inject core sample into a prepared LC jar. :grin:




I like that  :cool:


--------------------
HELP!!!!!!!!!


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OfflineFungi_x
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Registered: 08/17/03
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Re: To Clone or Not to Clone, Thats TeH Question [Re: Tippinthru]
    #5842500 - 07/10/06 03:36 PM (14 years, 9 months ago)

So you think its best to clone rather that use a MS-innoc?


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InvisibleTippinthru
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Registered: 04/07/05
Posts: 1,131
Loc: "The Garden"...
Re: To Clone or Not to Clone, Thats TeH Question [Re: Fungi_x]
    #5842546 - 07/10/06 03:51 PM (14 years, 9 months ago)

Quote:

Fungi_x said:
So you think its best to clone rather that use a MS-innoc?




It depends on your wants & skills.

Cloning will often get you good genetics.

Core sample clone will get you the best of several worlds.
It's quick, clean & effective.
A decent LC can inoculate a barrel of grain/wbs spawn.


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