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Invisiblejellyfish

Registered: 10/02/05
Posts: 7,457
Spore Sterility
    #5839096 - 07/09/06 02:58 PM (17 years, 6 months ago)

My friend has a spore print that we wants to start on some agar agar but is wondering how he can keep everything sterile because the spore print is obviously not 100% sterile. Is there anyway to sterilize it without hurting the spores or should he just try it and hope for the best?


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Offlinemikeownow
Humungus fungus

Registered: 09/01/05
Posts: 2,856
Loc: WA,USA
Last seen: 17 years, 3 months
Re: Spore Sterility [Re: jellyfish]
    #5839121 - 07/09/06 03:04 PM (17 years, 6 months ago)

Prints manage to stay steril quite well.


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Invisiblejellyfish

Registered: 10/02/05
Posts: 7,457
Re: Spore Sterility [Re: mikeownow]
    #5839159 - 07/09/06 03:10 PM (17 years, 6 months ago)

But are there any ways to sterilize like with alcohal? I hope my friend doesn't attempt it because he's gonna ruin the spores.


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Offlinemikeownow
Humungus fungus

Registered: 09/01/05
Posts: 2,856
Loc: WA,USA
Last seen: 17 years, 3 months
Re: Spore Sterility [Re: jellyfish]
    #5839163 - 07/09/06 03:12 PM (17 years, 6 months ago)

alchol will sterylize the surface of anything you put it on and when you sterylize something everything is gone,, meaning so will the mushrooms spores. Quite playing with it or u will get it contamed.


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No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.


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OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery

Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
Trusted Cultivator
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Re: Spore Sterility [Re: mikeownow]
    #5839260 - 07/09/06 03:41 PM (17 years, 6 months ago)

The word 'sterile' should not be used in conjunction with sporeprints. A sterile print would not produce anything, because it would be. . . sterile.

Prints need to be taken in a clean environment. A dirty print can usually be grown out, but it requires agar and isolation skills.

Alcohol is a sanitizer. It does not sterilize.
RR


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Invisiblefastfred
Old Hand
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Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
Re: Spore Sterility [Re: RogerRabbit]
    #5839364 - 07/09/06 04:12 PM (17 years, 6 months ago)

Use antibiotic agar.


-FF


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