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InvisibleBlutjager
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Registered: 06/11/06
Posts: 9,220
Contaiminated again
    #5836691 - 07/08/06 07:48 PM (17 years, 6 months ago)

OK,I just had to dump 10 Pints of wbs,so adding that to the 8 quarts of wbs and all 12 of my pf cakes that makes 30 jars that went bad.The pf went green and so did the quarts.The pints just never grew much of anything but they smelled horrible so I tossed them.They were done using 4 different lc's so I know they couldn't have all contaminated that way.I cant for the life of me figure out what I'm doing wrong.I Lysol the entire area I work around,I wear a face mask shower cap and rubber gloves and of coarse pressure cook everything the way I should.Is there something vitally important that I am missing and if so could someone please tell me what it is.This is getting so frustrating,I just nocced up 8 more quarts using foo's wbs tek and if this doesn't work I need to try something else.I have so much teen studd that it isn't even funny but I cant seem to get anything to colonize to spawn to it.Someone help me please.


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Invisiblefastfred
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Posts: 6,899
Loc: Dark side of the moon
Re: Contaiminated again [Re: Blutjager]
    #5836705 - 07/08/06 07:53 PM (17 years, 6 months ago)

> I cant for the life of me figure out what I'm doing wrong.

WBS sucks. It's too sweet and contams very easily. I suggest using rye grain. It's way, way better.


-FF


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Invisiblemonstermitch
Growing in Bags Doesn't Work

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Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay Flag
Re: Contaiminated again [Re: fastfred]
    #5836732 - 07/08/06 08:07 PM (17 years, 6 months ago)

is your mask medical grade?
if not it doesn't work.
using listerine under the mask?

lysol does not kill bacteria in the air.

you could be breathing bacteria through your mask.
this hits the needle along with mold spores,
and into the jars they go.

never let the needle touch open air if it is not still hot from
flame sterilization.

use an alcolhol swab around your needle at all times,
or inoculate with a hot needle and allow your LC to cool the needle
as you are inoculating.

LC's are often contaminated without the user knowing.
It is very possible your LC's are infected.

mold says your inoculation procedure is not good enough.
use a still air glove box next time.
clean surfaces with vinegar and alcohol.
for airborne bacteria, use oust.

many of us do just fine with wbs and millet.
rye is great, but you can use what you have.

read up.


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Offlinekristen
Burn out..don't fade away
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Registered: 04/19/06
Posts: 303
Last seen: 9 years, 25 days
Re: Contaiminated again [Re: fastfred]
    #5836773 - 07/08/06 08:24 PM (17 years, 6 months ago)

Yeah, because WBS sucks so much, most people don't use it in bulk cultivation at all. (Insert rolling eyes here)


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InvisibleBlutjager
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Registered: 06/11/06
Posts: 9,220
Re: Contaiminated again [Re: monstermitch]
    #5836789 - 07/08/06 08:27 PM (17 years, 6 months ago)

Yea I'm new to this and have been reading ALLOT.where is a good place to order rye online?are the rye berries from medium mania a good choice?.Being as new as I am I want to follow the advice of everyone and not change the tek and the tek I want to use is wbs too Tenn stud.Would I need to use the same amount of colonized rye? to poo or would the ratio stay the same? My mask says surgical grade and I do both flame and use a alcohol wipe on the needles before each jar.Is there any way to see if my LC is good before wasting a ton of jars.They always look all nice and whit and fluffy.Thanks guys


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Offlinesplifner180
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Registered: 03/24/06
Posts: 1,288
Loc: USA, East Coast
Last seen: 1 year, 3 months
Re: Contaiminated again [Re: Blutjager]
    #5836906 - 07/08/06 09:12 PM (17 years, 6 months ago)

I've found WBS to be dirt-simple to hydrate and spawn to hpoo. And I've yet to get a contam.

I'm not trying to shrug off your problem here. I'm just suggesting that something else is likely at fault.

splif


--------------------
First Grow: Ecuador -> LC -> HPoo/Straw -> Monotub
Build a Do-It-Yourself Magnetic Stirrer in thirty minutes with no money.


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OfflineComfortablyStond
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Registered: 03/15/06
Posts: 239
Loc: Psychedelphia, USA
Last seen: 13 years, 8 months
Re: Contaiminated again [Re: Blutjager]
    #5836908 - 07/08/06 09:12 PM (17 years, 6 months ago)

Save yourself some $$ and get your rye from the grocery or health food store. It can be 2x as expensive online with shipping costs.


--------------------
"It's not a war on drugs. It's a war on personal freedom. Keep that in mind at all times..." Bill Hicks


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Offlinejcee
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Registered: 06/06/06
Posts: 234
Loc: dallas tx
Last seen: 13 years, 11 months
Re: Contaiminated again [Re: ComfortablyStond]
    #5836983 - 07/08/06 09:40 PM (17 years, 6 months ago)

I found cracked corn to colonize 4 times as fast a wbs I decided I wont use anything else


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Invisibleliamtheloser
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Registered: 06/07/06
Posts: 1,453
Re: Contaiminated again [Re: jcee]
    #5837006 - 07/08/06 09:48 PM (17 years, 6 months ago)

speaking of wbs contams... i just got my first one on a half-pint of agaricus bisporis. happened when i shook at 30%, the lid wasn't tightened all the way, must have sucked some air in from the lid seam.


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OfflineSickShroomer
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Registered: 05/25/06
Posts: 361
Loc: Midwest
Last seen: 3 years, 14 days
Re: Contaiminated again [Re: jcee]
    #5837035 - 07/08/06 09:55 PM (17 years, 6 months ago)

Just use PF-TEK man.. It works like a charm.

As for the contam.. Are you steeping it for around 24hours first?

I made 12 PF jars and 0 contam.. Well 2 jars are not colonized the look healthy tho. Just slow colonizing phenotype or something, but maybe contam. But I've gotten fruit off of one already. Its a work in press.

Not saying anything but is your house really clean? That can be a big factor.. I mean cleaning the whole house top to bottom 1-2 beforehand. Then cleaning your work area when you do the PC, inocs, aka the work. Always flame you needle. And use alcohol to clean everything. Peroxide kills spore. Alcohol kill ever thing, so be care with it.

OK done babbling one this tread for now. Hope i helped a lil :smile:


--------------------
Going to miss the PNW gatherings.

I'm not here trying to make anyone feel stupid or to make myself feel good by putting ppl down. I am just here to help fellow shroomerites in there grows. I do this to keep my own knowledge fresh, and to help everyone succeed!


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Invisiblefastfred
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Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
Re: Contaiminated again [Re: SickShroomer]
    #5837125 - 07/08/06 10:24 PM (17 years, 6 months ago)

WBS works alright as spawn if you have good sterile practices. Otherwise it IS too sweet and very contam prone.

> Yeah, because WBS sucks so much, most people don't use it in bulk cultivation at all. (Insert rolling eyes here)

I guess it's cheap. Just posting my opinion. Props to the people that get it to work well. It's fun to watch how fast it colonizes, but IMHO it's too sweet and will contaminate. OTOH rye will work much better in the long run IMHO.


-FF


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