|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
ghostofbmarley
booyakasha



Registered: 06/27/06
Posts: 179
Loc: author's mind
Last seen: 16 years, 3 months
|
Sterilization of pf jars
#5829739 - 07/06/06 10:21 PM (17 years, 6 months ago) |
|
|
well, i got a bit distracted on my second round of pf jar sterilizations tonight and apparently let the water boil too hot as there was a nasty smell in my kitchen (apparently the washcloth charred) and no water left in the pots.
1st round: med hi heat to med boil, cover, turn down to med lo. allow low boil for 60 min. 60 min. cooling with lid on. 2nd round: like the first, except the med lo must not have been as low as the 1st as all of the water boiled away.
So, here is the question. It is unlikely that the water boiled away prior to T+50 based on the condition of the charred washcloth. Did I destroy the nutrients in these jars? They are going to sit for several days anyways, so I'll watch for contamination. But I am more worried about the nutrients being lost 
Any opinions? Thanks, ghost
|
liamtheloser
Advanced Idiot

Registered: 06/07/06
Posts: 1,453
|
|
the jars are probably fine, as long as they still have moisture inside of them... if they are dry, just use a liquid culture innoc and use a little more solution than normal... if the pf is actually BLACK, then no, dont use it.
--------------------
|
MasFina
Snow Shredder


Registered: 05/08/06
Posts: 788
Loc: Mountains
|
Re: Sterilization of pf jars [Re: liamtheloser]
#5829797 - 07/06/06 10:33 PM (17 years, 6 months ago) |
|
|
compare them with the first batch. Does the water content look the same? You most likely did lose some water, but nutrients are still there I'm sure. So you can just shoot em up with plenty of inoculant to rehydrate them some. Or, just sterilize some water and shoot it in there with an empty, sterile syringe prior to inocing.
-------------------- A Good Substrate: Poo With Extras Good Liquid Culture, Step by Step Timer Modification PM me if you are interested in buying 140ml syringes. $6 each + $7 shipping
|
ghostofbmarley
booyakasha



Registered: 06/27/06
Posts: 179
Loc: author's mind
Last seen: 16 years, 3 months
|
Re: Sterilization of pf jars [Re: MasFina]
#5830548 - 07/07/06 04:52 AM (17 years, 6 months ago) |
|
|
(This post has now been heavily edited.)
The jars basically look identical to the 1st batch. I guess it is a bit subjective though. Honestly, I can't see a difference if I mix and match and try to figure which is which (the 2nd batch has been marked to help differentiate.
EDIT: I have noticed some differences between the jars. Two pf jars that were sterilized during batch 2 in a smaller pot are noticably dryer than the other ten jars.
Now that I can see what a dried jar looks like, I can say the five batch 2 jars I was worried about are prob. OK as long as they don't show contamination. I can actually see a small difference at the bottom of the jars - batch 2 is a bit lighter at the bottom, however not on the sides.
The jars will be sitting on a shelf in the kitchen (indirect sunlight) for at least 4 days to make sure there is no visible contamination.
EDIT: While I have the audience, let me ask: the lids were not punctured or damaged prior to sterilization. Could they be reused if I redo the two driest jars?
Thanks for everyone's advice. ghost
Edited by ghostofbmarley (07/07/06 06:53 AM)
|
royer
±±±±±±±±±±

Registered: 05/15/06
Posts: 4,801
Loc: anywhere but here
Last seen: 4 years, 10 months
|
|
lol last night i did the same thing i was watching TV set the timer but never started it. about 1 1/2 hours later i said shit got up ran to the stove and there was no water in it just jars on the bottom sizzling. but they look just like my first batch and i can see some moister in them . i will give them i little more inoculate though.
-------------------- ================================================= if you have any questions please feel free to pm me , thx :-)
|
ghostofbmarley
booyakasha



Registered: 06/27/06
Posts: 179
Loc: author's mind
Last seen: 16 years, 3 months
|
Re: Sterilization of pf jars [Re: royer]
#5830603 - 07/07/06 05:32 AM (17 years, 6 months ago) |
|
|
royer9864 -
thanks for your post...makes me feel better. let's compare notes in a few weeks
-------------------- ~OM MANI PADME HUM~
|
royer
±±±±±±±±±±

Registered: 05/15/06
Posts: 4,801
Loc: anywhere but here
Last seen: 4 years, 10 months
|
|
sounds like a plan
-------------------- ================================================= if you have any questions please feel free to pm me , thx :-)
|
MasFina
Snow Shredder


Registered: 05/08/06
Posts: 788
Loc: Mountains
|
|
Not punctured? You should have had your 4 inoculation holes in there BEFORE sterilization. I apologize if I misread you. You can reuse lids with holes in them no prob. Even a little rust won't hurt. Since your not PCing though I would soak them in bleach water after washing with soap and water before reusing them AFTER they colonize and everything. I suggest getting at least an 8 quart PC (holds 7 pint jars). Was $40 at Sears for me. This will offer you much more flexibility and save you time and headaches in the future.
-------------------- A Good Substrate: Poo With Extras Good Liquid Culture, Step by Step Timer Modification PM me if you are interested in buying 140ml syringes. $6 each + $7 shipping
Edited by MasFina (07/07/06 10:06 AM)
|
ghostofbmarley
booyakasha



Registered: 06/27/06
Posts: 179
Loc: author's mind
Last seen: 16 years, 3 months
|
Re: Sterilization of pf jars [Re: MasFina]
#5831411 - 07/07/06 12:30 PM (17 years, 6 months ago) |
|
|
MasFina-
Well, that would be true if I was using the PF tek *exactly*. I am using shroom wizard which is pretty darn similar. It calls for the punctures to be made with a sterile nail just prior to 'noc (after sterilization) section 4A.
I read PF tek, and others, where it was suggested to punch first, but this calls for a little different strategy, and a slightly different substrate mixture.
More here if you are interested: http://www.shroomwizard.com/growguide.html
I actually thought I had seen another tek where you didn't poke holes until after sterilization, but I may be mistaken as I couldn't find anything to back that up. If I come across it again I will edit this post w/ an update.
The only variation I intend is to use sterilized shiat for the top layer when casing (as suggested in the tips section).
Lastly, I agree a PC will be a good investment for me to make. ghost
|
fastfred
Old Hand



Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
|
|
> It calls for the punctures to be made with a sterile nail just prior to 'noc (after sterilization) section 4A.
Very foolish and pointless. That's a good way to contam your jars, have pressure/vacuum problems, etc.. Stay away from PF tek knockoffs. Especially if they call themselves "shroomwizards". If you want to rely on magic then go with shroom wizard. Otherwise go with the tried and true method that started it all.
I also recommend drilling the holes. It's easier and the lids rust less and aren't as sharp so they're easier to wash. Drill one lid and make sure you get the hole placement right, then stack them up with the first one on top for a guide. It's also easier then punching them all separately.
> The only variation I intend is to use sterilized shiat for the top layer when casing (as suggested in the tips section).
That's not a casing layer. Casing layers are non-nutritive. Also stick with the basic PF tek your first time around. There'll be plenty of time for experimentation later.
> Lastly, I agree a PC will be a good investment for me to make.
That's the smartest thing I've heard all day. I'll give you 5 shrooms for that and for making an intelligent post.
-FF
|
ghostofbmarley
booyakasha



Registered: 06/27/06
Posts: 179
Loc: author's mind
Last seen: 16 years, 3 months
|
Re: Sterilization of pf jars [Re: fastfred]
#5832783 - 07/07/06 06:05 PM (17 years, 6 months ago) |
|
|
OK fastfred, it isn't too late for me to restart.... Can I still use a casing method sans shiat?
You have experience, and I don't, so i'll really consider starting over. I really only have about 5 hours of sterilization and some materials invested as of today.
Thanks, ghost
-------------------- ~OM MANI PADME HUM~
|
fastfred
Old Hand



Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
|
|
> Can I still use a casing method sans shiat?
You CAN, but I wouldn't. Unless you're really concerned about getting the best yield. From a dozen jars you'll get plenty of fruit just doing the standard PF tek. That's what I would do.
50/50 verm/peat is pretty popular to case with. 50/50+ is even better. You can also case with straight verm. That works just as well IME. Look up "dunk and roll" that's somewhat similar to casing and many people swear by it.
As far as the holes in the lids... It's a toss up. If you plan to reuse the lids for awhile I would consider taking them off and drilling them. If you got a cheap PC it would also pay to redo the jars.
Casing can cause you to run into problems so I wouldn't do it your first time. PF cakes have their own advantages, but it's more difficult to case them I would suppose. If you're going to case you should start with rye jars. If I were you I would just take your cakes and put them on perlite, that's the easiest and most foolproof method. Otherwise you would be breaking up your cakes and casing them, then waiting for them to recover and colonize the casing layer, then waiting for them to pin, all while hoping you don't run into contamination or overlay problems. It's far easier and probably a bit quicker to simply put them on perlite and wait for them to pin. Then you'll have plenty of spores to play with for your next round.
-FF
|
ghostofbmarley
booyakasha



Registered: 06/27/06
Posts: 179
Loc: author's mind
Last seen: 16 years, 3 months
|
Re: Sterilization of pf jars [Re: fastfred]
#5833351 - 07/07/06 09:05 PM (17 years, 6 months ago) |
|
|
fastfred,
i went ahead and aborted this run. it is a done deal and the 12 jars are soaking, waiting for a good wash after a supply run tomorrow. (may need new lids...) i am rinsed and dried the bands/lids and will wash and use them if i can.
since the jar lids were sealed rubber-seal down, i had to pry them off with a putty knife which did leave slight imperfections on the very edge of the lids. should i invest in new lids, or drill these puppies? i've gone to this length to do it right now lol! also, what size bit are you using -- i don't have a syringe to reference for sizing.
afa casing vs. the original method, i will study the original and see what is up. i see the wisdom in the "why fuck w/ success" points you have made.
many thanks. i think i needed an authority to tell me to punt  ghost
-------------------- ~OM MANI PADME HUM~
|
fastfred
Old Hand



Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
|
|
> should i invest in new lids, or drill these puppies?
They don't need to be airtight so I would say they are just fine to use. Some people even turn them upside down, although I don't recommend that. (rust reasons)
> what size bit are you using -- i don't have a syringe to reference for sizing.
Hmmm... I couldn't tell you. I'm sure you could find it if you search a bit. It's been a long time since I did PF style cakes. The exact size is not critical. As long as it is large enough for the needle. I like to drill them a bit large, then it's easier to move the needle around a bit to inoc a larger area. It would be hard to go wrong since if you used a bit smaller than a needle you would probably have a hard time drilling without breaking the bit.
> i see the wisdom in the "why fuck w/ success" points
Yep. The PF tek is what started it all. It's the most foolproof method around. It's best to save experimenting and the tougher teks for later because if you fail your second time who cares? You already know how to do it right and you can always fall back on the simple methods.
So study the PF tek, read the different versions and you should get a good idea exactly how to do things. If anything isn't totally clear ask about it. GL
-FF
|
|