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Invisiblebcfresh

Registered: 11/12/05
Posts: 61
Cakes, colonization issues..day 23
    #5828606 - 07/06/06 05:53 PM (17 years, 6 months ago)

I inoculated 8 PF style jars 23 days ago, and just in the last few days colonization has began to take place. The cakes have had very slow growth rates, which could be due to either an over-hydrated substrate, too much shaking of the jars, too coarse of vermiculite or possibly something else. I was going to toss the jars, but then a few days ago I noticed some mycellial growth. I've got a camera, I'll try and take some pictures. The strain is Orissa India...

What else could have caused slow colonization. I checked the water content of the substrate carefully and when I squeezed the stuff as hard as I could a couple drops of water would drip out. The vermiculite has some coarse peices in it, but for the most part it was similar to that shown in many illustrated guides(PF tek for simple minds etc). Also, I was under the impression that you could shake pf jars 5 days or so after inoculation, which is what I did with some of the jars. Was I misinformed?

Any help would be appreciated, I'll try to get pictures up asap  :smile:

PS sorry if there are many errors in grammer, or if any of this doesnt make sense..you know how it is  :syringe:


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bcf


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Offlinethenewguy05
The Mushroom Man
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Registered: 02/11/05
Posts: 2,123
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Re: Cakes, colonization issues..day 23 [Re: bcfresh]
    #5828683 - 07/06/06 06:09 PM (17 years, 6 months ago)

gas exchange. i don't know who told you to shake pf jars but you shouldn't. grain jars are the only jars that should be shaken; and it's to mix the colonized pieces of grain , from one solid clump into multiple inoculation point. you can't do that with a pf jar as the substrate is practically solid. good luck, hope this helped.


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InvisibleLateForTheFuture
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Registered: 02/24/03
Posts: 845
Re: Cakes, colonization issues..day 23 [Re: bcfresh]
    #5828851 - 07/06/06 06:44 PM (17 years, 6 months ago)

Quote:

bcfresh said:
I inoculated 8 PF style jars 23 days ago, and just in the last few days colonization has began to take place. The cakes have had very slow growth rates, which could be due to either an over-hydrated substrate, too much shaking of the jars, too coarse of vermiculite or possibly something else. I was going to toss the jars, but then a few days ago I noticed some mycellial growth. I've got a camera, I'll try and take some pictures. The strain is Orissa India...

What else could have caused slow colonization. I checked the water content of the substrate carefully and when I squeezed the stuff as hard as I could a couple drops of water would drip out. The vermiculite has some coarse peices in it, but for the most part it was similar to that shown in many illustrated guides(PF tek for simple minds etc). Also, I was under the impression that you could shake pf jars 5 days or so after inoculation, which is what I did with some of the jars. Was I misinformed?

Any help would be appreciated, I'll try to get pictures up asap  :smile:

PS sorry if there are many errors in grammer, or if any of this doesnt make sense..you know how it is  :syringe:




are you using an incubator?
:smile:


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Offlinefaceyneck
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Registered: 06/14/06
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Re: Cakes, colonization issues..day 23 [Re: LateForTheFuture]
    #5828940 - 07/06/06 07:04 PM (17 years, 6 months ago)

Yeah, use an incubator, and don't shake the jars. I haven't grown Orissa yet, so I don't know how long its supposed to take, but in the meantime, give 'em some r&r in a nice... warm... wet... man, I don't get laid nearly enough!


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Offlinecoda
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Re: Cakes, colonization issues..day 23 [Re: faceyneck]
    #5829528 - 07/06/06 09:28 PM (17 years, 6 months ago)

well, you most likely destroyed the dry verm barrier which is put in place to help prevent contams. If you squeeze as hard as you could and a little water came out id say you have an under hydrated substrate. You should give a GENTLE but firm squeeze (like one you'd use to squeeze out some toothpaste) and see a few drops of water fall. Underhydrated jars will colonize a lot slower then overhydrated ones because mushrooms require a good amount of water to propagate. Remember that a fruit body is made up of 90% water, you dont want soup for a substrate, but you need to provide adequate moisture so the colony can form and put off some nice fruits.

after 23 days and you're just seeing signs id whip up a new batch and start again as it's probably going to be another month before your current ones are done.


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Invisiblebcfresh

Registered: 11/12/05
Posts: 61
Re: Cakes, colonization issues..day 23 [Re: coda]
    #5830065 - 07/06/06 11:39 PM (17 years, 6 months ago)

I'm not using an incubator, but the place where the jars are stored has a temperature range of 75 - 80f.

Quote:

coda said:
well, you most likely destroyed the dry verm barrier which is put in place to help prevent contams.


 

Why? Because of the jar shaking? I don't see signs of contamination..yet :thumbdown:

Quote:

If you squeeze as hard as you could and a little water came out id say you have an under hydrated substrate.  You should give a GENTLE but firm squeeze (like one you'd use to squeeze out some toothpaste) and see a few drops of water fall.


 
Ohhh... thanks for the tip  :tongue2:

Quote:

after 23 days and you're just seeing signs id whip up a new batch and start again as it's probably going to be another month before your current ones are done.




I don't really want to to that, but if its what you suggest...What about if I were to put a few cc's of bottled water into each of the jars? Also, although these are the first signs of growth, I think its because the spores germinated in the center of the jar. I think the growth I'm seeing is the mycellium colonizing the last bit of the jar; the outside.

If I do throw these jars out and start anew..can I put these partially colonized jars outside?


--------------------
bcf


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Invisiblebcfresh

Registered: 11/12/05
Posts: 61
Re: Cakes, colonization issues..day 23 [Re: bcfresh]
    #5830465 - 07/07/06 03:13 AM (17 years, 6 months ago)

Coda, anyone?


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bcf


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OfflinePr0_X
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Re: Cakes, colonization issues..day 23 [Re: bcfresh]
    #5830532 - 07/07/06 04:22 AM (17 years, 6 months ago)

the mysteries of cultivation, to many factors man.

1, by squeezing out your substrate like that, its defiantly overly dry.
2, old spores
3, tempature fluxation

(if your temp keeps going up and down it will cause a delay in germination time and possibly stall future mycellium growth)

And yes, no need to shake the jars :smile:

I'm assuming your useing half pint jars...

40ml brown rice flour to 120ml's of vermiculite?

If you add in to much nutrients it will slow gown growth times, also, when you packed your jars did you leave your substrate airy? if you compressed it down into a brick like form chances are its not getting enough CO2/Gas exchange.


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OfflineMLBjammer
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Registered: 02/24/06
Posts: 560
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Re: Cakes, colonization issues..day 23 [Re: Pr0_X]
    #5830727 - 07/07/06 07:14 AM (17 years, 6 months ago)

A good rule of thumb with PF jars is that if you don't witness growth within a two-week period, start over. Chalk it up to experience, always have a backup plan, and move on. Day 23 should be about 100% colonization for most jars. GL.


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