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Offlinebarto
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culture jars colonizing faster than others?
    #5827549 - 07/06/06 12:49 PM (17 years, 6 months ago)

I just took a look at some of my jars and most do not have any white on them whatsoever while some are about 1/4 the way there. It's been 14 days since I put them in. Temperatures have been fluctuating but have not exceeded 81~ or dropped below 76~. I guess my question for you guys is do I risk contamination the longer they stay incubating? Where do I stand at this point?

I feel a little insane because I think I asked a similar quuestion like this last time I tried growing. It's just that I want things to go smoothly this time. I've never tried shrooms before.


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Offlinetallgreen
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Re: culture jars colonizing faster than others? [Re: barto]
    #5827591 - 07/06/06 01:02 PM (17 years, 6 months ago)

After 14 days I would expect to see some growth. But I have had jars that sprung up about 2 weeks after showing nothing. I would give them another week or so. I like using LC cause if you use LC to inoculate and you still don't see something after 14 days, you can be pretty sure it didn't work. Spore inoculation seems to be less predictable.


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Offlinebarto
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Re: culture jars colonizing faster than others? [Re: tallgreen]
    #5843204 - 07/10/06 04:35 PM (17 years, 6 months ago)

it seems that some are moving along faster than others now. only one is close to being finished.

question: would a slight greying be considered contam? its off colored from the orginal substrate. im hoping this is just caused by teh water and heat buildup or something.


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Invisibledecepticon
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Re: culture jars colonizing faster than others? [Re: barto]
    #5843231 - 07/10/06 04:41 PM (17 years, 6 months ago)

white is the only good color.

if you used spores, it will take the jars different amounts of time to colonize.

spores have to germinate first before they begin their growth.
when the ones that are ahead are done,
stick them in the fridge to wait for the others.
just remove them one day before use.
if you want them all together that is.


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Offlinebarto
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Re: culture jars colonizing faster than others? [Re: decepticon]
    #5887553 - 07/22/06 01:20 PM (17 years, 6 months ago)

i just checked the incubation chamber and there is one that appears to be fully colonized. question: do i need to unscrew the lid to make sure the very top is white also? (looking through the holes, it look like mycelium hasnt spread on to the very top layer). help on this step would be appreciated. im ready to move on.

how long should i wait until the mycelium starts producing pin heads in the jar? would cold shocking them over night work?
unscrewing the lid probably wouldnt be a good idea/


Edited by barto (07/22/06 01:55 PM)


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Offlinestormhalter
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Re: culture jars colonizing faster than others? [Re: barto]
    #5887736 - 07/22/06 02:25 PM (17 years, 6 months ago)

Do a search on cold shocking. As for the very top not being fully colonized, that's probably the opt layer of your vermiculite you're referring to right? That gets flipped to be the


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OfflineYamidude
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Re: culture jars colonizing faster than others? [Re: stormhalter]
    #5887783 - 07/22/06 02:43 PM (17 years, 6 months ago)

Quote:

stormhalter said:
Do a search on cold shocking. As for the very top not being fully colonized, that's probably the opt layer of your vermiculite you're referring to right? That gets flipped to be the


cold shocking is completely unnecessary.


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OfflineRogerRabbitM
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Re: culture jars colonizing faster than others? [Re: Yamidude]
    #5888053 - 07/22/06 04:26 PM (17 years, 6 months ago)

It also would help if you said what is IN the jar. Don't unscrew the lid on a grain jar. If it's a brf jar, you should have a dry vermiculite barrier on top that serves as a filter, so you can. However, the dry verm layer rarely colonizes unless you got it wet, in which case it's useless as a filter.

Cold shocking is for cold weather edibles, but it's never done before you're ready to initiate fruiting, which you're not. Tropical species do not benefit from a cold shock, so it's important that you study up on the species you grow and determine its parameters.
RR


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Offlinebarto
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Re: culture jars colonizing faster than others? [Re: RogerRabbit]
    #5892982 - 07/24/06 12:24 AM (17 years, 6 months ago)

its brown rice anf vermiculite. like i said one jar has completely colonized but has yet to produce any pin heads. is it an absolute that i wait for heads to form in the jar before transferring them to the terrarium? many grow teks differ thats why im asking these type of questions.


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Offlinebarto
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Re: culture jars colonizing faster than others? [Re: barto]
    #5898069 - 07/25/06 12:32 PM (17 years, 6 months ago)

here's another question for y'all. ive got pin heads poking out of a culture jar that hasnt completely colonized white yet. should i immediately put it in the growing chamber.

p.s. - ive also got a culture jar that has colonized fully but no pin heads. what should i do on both counts? thanks


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Re: culture jars colonizing faster than others? [Re: barto]
    #5898434 - 07/25/06 02:21 PM (17 years, 6 months ago)

They both need to be fully colonized before you birth them. If the pins look like they're going to rot before that happens, birth the cake and scrape away the uncolonized substrate before you put it in the FC.

Next time don't let them get so much light.


-FF


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Offlinebarto
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Re: culture jars colonizing faster than others? [Re: fastfred]
    #5898498 - 07/25/06 02:39 PM (17 years, 6 months ago)

yes i will remember that, fred. ive been having to keep the top of my incubator slightly off because i cant maintain a steady temp to my liking. for future reference..does anyone know why brown rice/verm calls for 75 -81 degrees and WBS up to 85 or something like that?


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Invisiblefastfred
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Re: culture jars colonizing faster than others? [Re: barto]
    #5898590 - 07/25/06 03:06 PM (17 years, 6 months ago)

85F is the optimal incubation temp. 1/2 pint BRF cakes should be incubated at 83-84F and bulk grains in quart jars should be incubated at around 80-83F. Myc generates some heat while growing so that's why you want to incubate at less than 85F. Larger jars will throw off more heat and reach higher temps at their core, so you need to drop the temp a couple degrees.


-FF


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Offlinebarto
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Re: culture jars colonizing faster than others? [Re: fastfred]
    #5909779 - 07/28/06 06:52 PM (17 years, 6 months ago)

using a cool 25" fluorescent light. how long should i keep in on under the terrarium during the day? also, is it a must that all colonized jars develope pins in their jars before transplanting them to the fruiting chamber?


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Offlinebarto
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Re: culture jars colonizing faster than others? [Re: barto]
    #5912261 - 07/29/06 02:44 PM (17 years, 6 months ago)

bump


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Invisiblefastfred
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Re: culture jars colonizing faster than others? [Re: barto]
    #5913475 - 07/29/06 11:24 PM (17 years, 6 months ago)

> is it a must that all colonized jars develope pins in their jars before transplanting them to the fruiting chamber?

No that is totally wrong. You should never have pinning until your substrate is in the fruiting chamber. It will screw your first flush up if you get pinning in the jars.


-FF


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Offlinebarto
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Re: culture jars colonizing faster than others? [Re: fastfred]
    #5932419 - 08/04/06 07:04 PM (17 years, 5 months ago)

ive grown impatient. only two pins have sprouted to anything worth mentioning. the rest of the cultures ive put in there have developed nothing. i guess im not humidifying the growing chamber enough. next i will move on to straight up casing. my question is about the two pins. -the shafts are solid white and only one of the heads have opened up. the other looks like an abort. are these safe to eat?

also i would like for you guys to guess the weight of them by looking at the culture. my plan is to hope they are at least 4 grams and consume. does anyone have any safe methods of uploading pics to shroomery?


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Offlinehawksapprentice
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Re: culture jars colonizing faster than others? [Re: barto]
    #5933197 - 08/04/06 11:25 PM (17 years, 5 months ago)

Dude, no one is going to be able to tell you the weight of the mushroom by looking at a picture of it. Anyone who does claim so is lying. Get a scale if you want to weigh out your mushrooms. Also you have to keep in mind the difference between wet weight and dry weight in mushrooms.


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