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DriAno
student


Registered: 03/01/06
Posts: 149
Last seen: 16 years, 1 month
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colonization stopt
#5825080 - 07/05/06 07:54 PM (17 years, 6 months ago) |
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i have a few jars which were inoculated using pf tech and p mexicana. 8 weeks ago , at about the 6th week colonization stopped at 45% and the Mic has a yellowish hue. what caused this? and is there anything i can do to trigger Mic growth? if not could i birth them? any help is greatly appreciated. thanks everyone
-------------------- ...................................................................Smile. Its not that hard. Just squeeze ur But cheecks. SEE!!! ...................................................
Edited by DriAno (07/05/06 07:59 PM)
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hyphae
born to grow


Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 8 months
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Re: colonization stopt [Re: DriAno]
#5825125 - 07/05/06 08:03 PM (17 years, 6 months ago) |
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Do you have any tape on the jars? If so remove it and flip the jars upside down.
-------------------- Getting the most out of your casings!, A pinning strategy. Oyster Shell "Flour" $2 for 1lb. a hell of a deal Not what is overlay but rather what overlay is Gas Exchange vs. FAE "We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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Schwip
Never sleeps.


Registered: 06/27/05
Posts: 3,937
Last seen: 11 years, 2 months
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Re: colonization stopt [Re: DriAno]
#5825138 - 07/05/06 08:07 PM (17 years, 6 months ago) |
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Flipping the jars may help.
Did you use filler patches or just verm on top with holes?
Maybe your water ratio was too low? Do things look really dry in the jars?
also *stopped
-------------------- -------------------------------- " If the sky were to suddenly open up there would be no law. There would be no rule. There would only be you and your memories... the choices you've made, and the people you've touched. If this world were to end there would only be you and him and no-one else. " .............. "MAN! You know there aint no such thing as left over crack!"
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DriAno
student


Registered: 03/01/06
Posts: 149
Last seen: 16 years, 1 month
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Re: colonization stopt [Re: Schwip]
#5825162 - 07/05/06 08:14 PM (17 years, 6 months ago) |
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i used verm at the top with a foil lid, and i already fliped the jars last week ,to see if mabe the moisture that had settled on the bottom would run down the sides of the jar, how ever there are moisture drops that will run down the side of the jar (on the inside). the jars do not look dry i was thinking of mabe re enoculating , adding an extra cc of spores could that work?
-------------------- ...................................................................Smile. Its not that hard. Just squeeze ur But cheecks. SEE!!! ...................................................
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DriAno
student


Registered: 03/01/06
Posts: 149
Last seen: 16 years, 1 month
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Re: colonization stopt [Re: DriAno]
#5825166 - 07/05/06 08:15 PM (17 years, 6 months ago) |
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hey Schwip, what strand did u use?
-------------------- ...................................................................Smile. Its not that hard. Just squeeze ur But cheecks. SEE!!! ...................................................
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Chrissss
poke smoter
Registered: 02/12/06
Posts: 227
Last seen: 17 years, 1 month
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Re: colonization stopt [Re: DriAno]
#5825479 - 07/05/06 09:47 PM (17 years, 6 months ago) |
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I'm sorry but i have too. spell check is right at the bottom
-------------------- http://www.laserspectacular.com/index.htm This is a laser show with pink floyd music. If it comes near you GO! it is amazing
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Schwip
Never sleeps.


Registered: 06/27/05
Posts: 3,937
Last seen: 11 years, 2 months
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Re: colonization stopt [Re: DriAno]
#5826327 - 07/06/06 01:52 AM (17 years, 6 months ago) |
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strain will not make all that big of a difference.
can you detail exactly how you went about getting to the point you are at now?
did you use brown rice flower? how much water? was the syringe from a vendor? do the jars smell funny(bad)? How long ago did you inoculate? What temperature are you keeping them at? Did you use a PC to sterilize?
Cant just guess whats wrong here 
Also don't bother inoculating the same jars again. If you still have spore water try again with fresh jars(if these are truly fucked)
-------------------- -------------------------------- " If the sky were to suddenly open up there would be no law. There would be no rule. There would only be you and your memories... the choices you've made, and the people you've touched. If this world were to end there would only be you and him and no-one else. " .............. "MAN! You know there aint no such thing as left over crack!"
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hyphae
born to grow


Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 8 months
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Re: colonization stopt [Re: Schwip]
#5826721 - 07/06/06 06:12 AM (17 years, 6 months ago) |
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Crack you jars lids 1/4-1/2 turn (no more) and leave flipped. Your myc is suffering from a lack of gas exchange more than likely or you have a bacterial infection. Theres nothing you can do about contams at this point but if it is a lack of gas exchange you can possible still save them if you act fast and follow my advice. BTW flipping does help when used with adequate gas exchange. GL bro
-------------------- Getting the most out of your casings!, A pinning strategy. Oyster Shell "Flour" $2 for 1lb. a hell of a deal Not what is overlay but rather what overlay is Gas Exchange vs. FAE "We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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DriAno
student


Registered: 03/01/06
Posts: 149
Last seen: 16 years, 1 month
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Re: colonization stopt [Re: hyphae]
#5827045 - 07/06/06 09:35 AM (17 years, 6 months ago) |
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hey thanks, ill try and see what happens.
-------------------- ...................................................................Smile. Its not that hard. Just squeeze ur But cheecks. SEE!!! ...................................................
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MLBjammer
Invitro Freak


Registered: 02/24/06
Posts: 560
Loc: Southeastern USA
Last seen: 7 years, 14 days
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Re: colonization stopt [Re: DriAno]
#5827057 - 07/06/06 09:39 AM (17 years, 6 months ago) |
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Eight weeks is a long, long time. I would start something else and either scrap those cakes or start an outdoor pot. Like Hyphae said, you likely have a major bacterial infection. GL.
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DriAno
student


Registered: 03/01/06
Posts: 149
Last seen: 16 years, 1 month
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Re: colonization stopt [Re: MLBjammer]
#5828196 - 07/06/06 04:05 PM (17 years, 6 months ago) |
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ahhhhhhhhh shit. ok ill take a look at some out door growing. thanks people
-------------------- ...................................................................Smile. Its not that hard. Just squeeze ur But cheecks. SEE!!! ...................................................
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hyphae
born to grow


Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 8 months
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Re: colonization stopt [Re: DriAno]
#5829531 - 07/06/06 09:29 PM (17 years, 6 months ago) |
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After all this time you owe it to yourself to wait a couple of days to see if they can recover. You should see a noticeable amount of growth within two days FYI
-------------------- Getting the most out of your casings!, A pinning strategy. Oyster Shell "Flour" $2 for 1lb. a hell of a deal Not what is overlay but rather what overlay is Gas Exchange vs. FAE "We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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DriAno
student


Registered: 03/01/06
Posts: 149
Last seen: 16 years, 1 month
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Re: colonization stopt [Re: hyphae]
#5833284 - 07/07/06 08:30 PM (17 years, 6 months ago) |
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hey thnks!
-------------------- ...................................................................Smile. Its not that hard. Just squeeze ur But cheecks. SEE!!! ...................................................
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