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Invisiblepsychocactustrip
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EXPERIMENT: liquid culture: karo: optimal amount: 5, 10, or 15ml per 100ml water
    #5825065 - 07/05/06 07:51 PM (17 years, 10 months ago)

disclaimer: since this experiment was informal, the writeup will be as well. it is meant to give ideas, not state facts. you are encouraged to do similar experiments and see if they produce similar results (if u try something similar post in this thread!)
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liquid culture: karo: optimal amount: 5, 10, 15ml per 100ml water

below is an experiment with all conditions the same except the amount of karo. it was blind; the jars were marked but not looked at until after observations were made.

100 ml water per jar

2 jars with 5 ml karo
2 jars with 10 ml karo
2 jars with 15 ml karo
(yes my foaf realizes that 2 of each isnt very many, he will probably use more of each next time to make for a better experiment)

pictures weren't coming out well, so ill just describe them.

15 was too much, there was significantly less mycelium.

5 and 10 had about the same, but the 10 seemed to be fuller and more rhizomorphic.

next experiment will test optimal amount between the 5-10 range.


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The above is pure fiction

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OfflineAA2277
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Re: EXPERIMENT: liquid culture: karo: optimal amount: 5, 10, or 15ml per 100ml water [Re: psychocactustrip]
    #5825332 - 07/05/06 09:06 PM (17 years, 10 months ago)

Wouldn't that value very from strain to strain?


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InvisibleMezcal
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Re: EXPERIMENT: liquid culture: karo: optimal amount: 5, 10, or 15ml per 100ml water [Re: psychocactustrip]
    #5825420 - 07/05/06 09:30 PM (17 years, 10 months ago)

Good idea!

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Invisiblefastfred
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Re: EXPERIMENT: liquid culture: karo: optimal amount: 5, 10, or 15ml per 100ml water [Re: Mezcal]
    #5826321 - 07/06/06 01:46 AM (17 years, 10 months ago)

2-4% sugars in media is considered optimal for quite a wide range of species.

corn syrup = 19.7% water, so 5ml is going to be about 4g sugars

So that would be 5ml/100ml = ~4% sugars in 100ml water.

So it might be more optimal to adjust your experimental range to 3-10ml per 100ml. I would imagine the upper limit to be softer and more variable than the lower limit. It would also be better to establish an optimal range, because you probably aren't going to find any exact optimal point. Also as the sugars are consumed it's going to change the concentration on you leading to a higher range the longer you let the experiment go. Doing it on agar would be a better way to keep things constant and also to measure growth rate.

Good experiment though. GL.


-FF

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Invisiblepsychocactustrip
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Re: EXPERIMENT: liquid culture: karo: optimal amount: 5, 10, or 15ml per 100ml water [Re: fastfred]
    #5828969 - 07/06/06 07:11 PM (17 years, 10 months ago)

fastfred,

not saying ur wrong or anything about the percent, but then why would the 10ml turn out fuller and more rhizomorphic than the 5ml? i guess it could have just been a fluke since there was only 2 jars of each test condition, but it definitely seemed like the mycelium was better in the 10ml.


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Invisiblefastfred
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Re: EXPERIMENT: liquid culture: karo: optimal amount: 5, 10, or 15ml per 100ml water [Re: psychocactustrip]
    #5832945 - 07/07/06 06:43 PM (17 years, 10 months ago)

I'm just saying what the consensus is on carbon source concentration. I've OCRd a section from "Genetics and Breeding of Edible Mushrooms" for you. Although they cite 2%, 2-4% is usually cited for most media types. There are also other factors to consider besides initial growth. It's better to have the sugar run out and growth slow rather than have the myc end up drowning in it's own piss. There are other factors and reasons, but I'm no expert.


-FF

From: "Genetics and Breeding of Edible Mushrooms"

Nutritional Requirements

The nutritional requirements for mycelial growth are relatively simple. Since the fungi are heterotrophic organisms, they must be supplied with a source of carbon. While many carbon sources may be used, individual species commonly have a preference, but among the simple sugars glucose is most frequently preferred, and in amounts of approximately 2%. In addition to the simple sugars, polysaccharides may provide carbon for the fungi. Such polysaccharides are the usual source of carbon for fungi in nature, and the insoluble polysaccharides, such as cellulose, are broken down by extracellular enzymes to simpler, soluble units which are then taken into the fungal hyphae by absorptive (osmotrophic) nutrition. Under certain conditions a number of organic compounds (alcohols, organic acids, polycyclic compounds, and amino acids) may also provide carbon for mycelial growth. It is worth mentioning at this point that a mixture of sugars may give greater growth than simply the summation of growth to be obtained by each separately (Horr, 1936). On the other hand, another frequent observation in studies of carbon nutrition is that when a fungus is supplied with a mixture of carbon sources, it may use one preferentially over the others. The matter of concentration of carbon source is also important in determination of the effectiveness of promotion of growth. This has been shown in Copri;uis lagopus (= C. cinereus) by Moore (1969) with the demonstration that growth on sucrose is negligible at low concentrations but occurs at higher sugar concentrations.
Obviously, all organic compounds (carbohydrates, amino acids, lipids, nucleic acids) require carbon in their skeletal framework but it should not be overlooked that the carbon compounds supplied to fungi also provide the energy required for the organism’s metabolic activities.

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Offlinedebianlinux
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Re: EXPERIMENT: liquid culture: karo: optimal amount: 5, 10, or 15ml per 100ml water [Re: fastfred]
    #5843562 - 07/10/06 05:57 PM (17 years, 10 months ago)

I have read multiple sources that I can't be arsed to site that have specified 2-4% with 4% being the more popular figure.

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Offlineaccesstwo
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Re: EXPERIMENT: liquid culture: karo: optimal amount: 5, 10, or 15ml per 100ml water [Re: debianlinux]
    #5857148 - 07/14/06 06:51 AM (17 years, 10 months ago)

isn't 4% in weight the stanard anyway? 4/96ml

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