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OfflineShampioenier
Storm in aTeaCup
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Registered: 07/29/05
Posts: 260
Loc: Milky Way Galaxy
Last seen: 17 years, 6 months
Re: or, in this case, the best 20 out of 20 billion? ... [Re: Shampioenier]
    #5883691 - 07/21/06 05:49 AM (17 years, 6 months ago)

sorry, that's aigi-herb.com

They sell gummi berry juice ingredients too :smile: :mushroom2:


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Offline_OttO_
Over Stimulated
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Registered: 06/01/05
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Re: NO!!! listen! [Re: fastfred]
    #5884466 - 07/21/06 01:31 PM (17 years, 6 months ago)

Quote:

fastfred said:
> If you have 200 athletes, you will not have any faster athlete then if you take the fastest 20 of those 200 and had a race.

Your analogy doesn't make much sense.


-FF




It makes perfect sense from where Im sitting.

Quote:

fastfred said:
How do you pick the 20 best spores out of 200?




By using techniques such as streaking on agar plates, and isolating the healthiest looking substrains, then fruiting each one individually to see what produces the best fruit bodies - then printing those specimines?


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Invisiblefastfred
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Registered: 05/17/04
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Re: NO!!! listen! [Re: _OttO_]
    #5886119 - 07/21/06 11:10 PM (17 years, 6 months ago)

Sure, I suppose that would work to some degree. I thought we were talking in the context of MSI though.

IMO there is no real disadvantage to using a high spore concentration in MSIs. There are some potential advantages though.

There is a problem getting much actual competition to happen though. The spores that germinate faster have the biggest advantage. If you have a high spore concentration you are likely to have a good range of genetics germinating at about the same time, whereas if you had a lower spore concentration you might not have very many germinating at the same time.

I guess the whole thing is debatable, but if your interested in more competition then more spores is the way to go. I don't think you can really question that.

Optimally you would dilute the spores and spread plate or overlay them on agar and isolate monokaryotic strains, analyze those, pick various mating combinations, then analyze the dikaryotic mycelia from those matings, then test them on actual substrate, then chose master strains to use.

That's a lot of work though and if you have the time and resources to do all that you are probably going to use your advanced breeding program for strain improvement and would likely be interested in the commercial aspects of it. I don't think that there are too many people like that here.

> the best spores and hyphaes genetic makeup takes over the colony,

"Best" is a subjective term. The fastest growing spores and mycelia will out colonize the slower ones. That doesn't necessarily mean that they are better in any other way. They might also be faster on your spawn, but slower on the substrate or faster on agar and slower on the spawn.

IMO for MSI you can't go wrong with more spores. You'll have more genetic variation and selection pressures will result in better suited mycelia colonizing the majority of the substrate.

From what it sounds like you're going to have contamination problems the way you did it though.


-FF


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Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
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Last seen: 11 years, 26 days
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Re: NO!!! listen! [Re: fastfred]
    #5902310 - 07/26/06 04:19 PM (17 years, 6 months ago)

Isolates would be like picking the best runners of the crowd.

I did not start the stupid analagy..


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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