|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
DreadedOne
Stranger
Registered: 07/05/06
Posts: 10
Last seen: 7 years, 9 days
|
Spore inoculation *DELETED*
#5822699 - 07/05/06 06:55 AM (17 years, 6 months ago) |
|
|
Post deleted by DreadedOneReason for deletion: ....
|
Shampioenier
Storm in aTeaCup


Registered: 07/29/05
Posts: 260
Loc: Milky Way Galaxy
Last seen: 17 years, 6 months
|
Re: Spore inoculation [Re: DreadedOne]
#5822711 - 07/05/06 07:09 AM (17 years, 6 months ago) |
|
|
naw thats fine ive been there done that. cant you afford a hepa. Just be very careful. I can give you some info to prep yourself first. be VERY slow. no hurry.
|
Shampioenier
Storm in aTeaCup


Registered: 07/29/05
Posts: 260
Loc: Milky Way Galaxy
Last seen: 17 years, 6 months
|
|
first. ok so you stuff is sterilized out of Pc right. Toss a cloth over the pressure cooker while its cleaning. This cloth should be soaked in 10 % bleach water solution. When you open the pressure cooker to put the jars you want to inoculate on the ovenrack, that is contamination time. So maybe you can get someone to help you, someone also who took a shower and also clean short shirt, washed arms with antibacterial soap (preferrably have/ wear surgical gloves (cheap), wash gloved hands in bleach water tub (10%) ok. So while person no 2 holds put in front of open warm air blowing out oven you turn the lid and out under the bleach cloth covering it directly place the jars on the oven rack. BUT!!!! WAIT!!! BE SURE TO FIRST STERILIZE THE OVEN ALSO. KEEP IT ON FOR LIKE AN HOUR ON REALLY HOT< AND DO THE TRANSFERS when its cooled down somewhat to tolerable levels. trust me. ive SINGED my arms in my dam oven that aint fun. So now your jars on properly on the prepared rack. Your syringe, keep it on your side of the oven while you inoculate, downstream. If you can without damaging it, brush the outside of the syringe off with the bleach cloth. Open the needle and have a flame ready and sterilize the needle red hot, squirt out a drop and inject, you will hear it sizzle; then the anvil is hot inoculate. What type of jars u use? take a shower. 1) short sleeve shirt.
|
DreadedOne
Stranger
Registered: 07/05/06
Posts: 10
Last seen: 7 years, 9 days
|
|
1/2 pint ball jelly jars
|
Shampioenier
Storm in aTeaCup


Registered: 07/29/05
Posts: 260
Loc: Milky Way Galaxy
Last seen: 17 years, 6 months
|
Re: Spore inoculation [Re: DreadedOne]
#5822742 - 07/05/06 07:48 AM (17 years, 6 months ago) |
|
|
what is the growing medium whats in em? How did you test the moisture capacity, howd u prep em?
|
Shampioenier
Storm in aTeaCup


Registered: 07/29/05
Posts: 260
Loc: Milky Way Galaxy
Last seen: 17 years, 6 months
|
|
also, get something to cover u mouth, like a mouthmask from the pharmacy their dirt cheap really as cheap as the surgical gloves. try not to breath to hard slow calm little breaths... :-D dont want too many molecules flyin round, u know.
|
DreadedOne
Stranger
Registered: 07/05/06
Posts: 10
Last seen: 7 years, 9 days
|
|
I used brown rice and vermiculite as a substrate using the ratio's provided by the pk tek, sealed them with aluminum foil and boiled them for an hour and 10 minutes. Im not sure how much water evaporated while they were being boiled but there was originally 1/4 cup of water in each of the jars
|
royer
±±±±±±±±±±

Registered: 05/15/06
Posts: 4,801
Loc: anywhere but here
Last seen: 4 years, 10 months
|
|
dont have to go ot that extent if you dont want to just wash your hands good and spray the air with oust open the cold pressure cooker and inject spores and put filterdisk or micropore tape on holes then cover in tin foil and inncubate. the other stuff is overkill but you can do it if you want.
-------------------- ================================================= if you have any questions please feel free to pm me , thx :-)
|
DreadedOne
Stranger
Registered: 07/05/06
Posts: 10
Last seen: 7 years, 9 days
|
Re: Spore inoculation [Re: DreadedOne]
#5822755 - 07/05/06 08:00 AM (17 years, 6 months ago) |
|
|
I then got impatient and inoculated them before I got a chance to read your first post, a stupid mistake. I did it on my counter top with minimal contamination prevention, I doubt they will turn out okay:(
|
royer
±±±±±±±±±±

Registered: 05/15/06
Posts: 4,801
Loc: anywhere but here
Last seen: 4 years, 10 months
|
Re: Spore inoculation [Re: royer]
#5822756 - 07/05/06 08:01 AM (17 years, 6 months ago) |
|
|
i make jars every 10 days this way and i dont even use a pressure cooker i just boil with a lid and then i do what i just said and with in 15 25 days jars are done. i have had not one contam jar yet..
-------------------- ================================================= if you have any questions please feel free to pm me , thx :-)
|
royer
±±±±±±±±±±

Registered: 05/15/06
Posts: 4,801
Loc: anywhere but here
Last seen: 4 years, 10 months
|
Re: Spore inoculation [Re: royer]
#5822757 - 07/05/06 08:02 AM (17 years, 6 months ago) |
|
|
you will be supprised the dark kills a lot of contams
-------------------- ================================================= if you have any questions please feel free to pm me , thx :-)
|
malarki
Master Jack ofAll Trades,Realist


Registered: 06/17/06
Posts: 200
Loc: Ashittown, USA
|
Re: Spore inoculation [Re: royer]
#5822761 - 07/05/06 08:05 AM (17 years, 6 months ago) |
|
|
Quote:
royer9864 said: don't have to go ot that extent if you dont want to just wash your hands good and spray the air with oust open the cold pressure cooker and inject spores and put filterdisk or micropore tape on holes then cover in tin foil and inncubate. the other stuff is overkill but you can do it if you want.
I agree also you want to make sure the jars have cooled.. I made the mistake once of inoculating to soon and high heat can kill spores.
http://www.shroomery.org/index.php/par/7919 Look at this tek.. it's helpful I inject mine in the kitchen w/ out using the oven as a flow hood. I am sure they will be fine as long as you let it cool
|
Shampioenier
Storm in aTeaCup


Registered: 07/29/05
Posts: 260
Loc: Milky Way Galaxy
Last seen: 17 years, 6 months
|
Re: Spore inoculation [Re: malarki]
#5822768 - 07/05/06 08:09 AM (17 years, 6 months ago) |
|
|
yep overkill me gree. Cos me almost couldnt breethe for coupla days. but that is also not my preference. a hepa filter really is da bomb. but working with an oven you really have to be totally in control and that well that way will work guaranteed.
|
royer
±±±±±±±±±±

Registered: 05/15/06
Posts: 4,801
Loc: anywhere but here
Last seen: 4 years, 10 months
|
|
this is over kill but it is my opinion and then you agree with us and say something different. ?? if you are singing your hairs you oven has to be hotttt and that will def kill spores. lmao
-------------------- ================================================= if you have any questions please feel free to pm me , thx :-)
|
Shampioenier
Storm in aTeaCup


Registered: 07/29/05
Posts: 260
Loc: Milky Way Galaxy
Last seen: 17 years, 6 months
|
Re: Spore inoculation [Re: royer]
#5822777 - 07/05/06 08:15 AM (17 years, 6 months ago) |
|
|
haha. I know man. that was my first time. It was like a nasty assed scab for like 3 weeks. But I usually take my jars into my steril or filter patc bags into sterile environ while steaming so nothing lands on them... also i presterilize my substrate in jars before i transfer them still hot into filterpatch bags so that nothing can land on the steamy stuff u know, then i pressure cook it again. sorry i just dont want any more problems. i make sure its all done good and proper, like a doctor...
|
Shampioenier
Storm in aTeaCup


Registered: 07/29/05
Posts: 260
Loc: Milky Way Galaxy
Last seen: 17 years, 6 months
|
|
ive tried about every trick in the book and my present technique is infallible it works for me. its just maybe my luck maybe im just a dirty bastard!!!! ;D
|
royer
±±±±±±±±±±

Registered: 05/15/06
Posts: 4,801
Loc: anywhere but here
Last seen: 4 years, 10 months
|
|
the bags are to my knowledge autoclave able and are able to be pc'd right?.
-------------------- ================================================= if you have any questions please feel free to pm me , thx :-)
|
Shampioenier
Storm in aTeaCup


Registered: 07/29/05
Posts: 260
Loc: Milky Way Galaxy
Last seen: 17 years, 6 months
|
Re: Spore inoculation [Re: royer]
#5822798 - 07/05/06 08:38 AM (17 years, 6 months ago) |
|
|
yup. goto fungi.com
they rock. of course tossin in the pre sterilized WBS... you take some ordinary masking tape and close the top off too you dont need a plastic bag sealing machine. This way you can reuse the bags also... after innoculating. Rub the spot with alcohol, flame needle, inoculate, but have a piece of band aid to seal the wound like if you scratched u arm band aid the bag with breathable patches they are the cheapest type bandage bandaid stuff anyways and they presterilize cause it was meant for peoples wounds. Then u r set...
|
malarki
Master Jack ofAll Trades,Realist


Registered: 06/17/06
Posts: 200
Loc: Ashittown, USA
|
Re: Spore inoculation [Re: royer]
#5822799 - 07/05/06 08:38 AM (17 years, 6 months ago) |
|
|
-------------------- ~*~I'm also an educated guesser, and knowledge seeker If I haven't done it, I know someone who has. IF I don't know, I know someone who does! I am a realist so deal with it, if you don't like it you can choose to not read it!
|
Shampioenier
Storm in aTeaCup


Registered: 07/29/05
Posts: 260
Loc: Milky Way Galaxy
Last seen: 17 years, 6 months
|
|
of course i do this in front of my trusty hepa filter. and of course i spray the air with swimmin pool hot water solution, i got my gas mask (good one) and swimmin goggles, surgical glovs, and am waiting for my sani-mate air sanitizer (32$ from natural-living.com
and of course also i am waiting for my thermostat controller from bigappleherp.com a controlling thermostat that u plug on u heater so if it gets too hot it switches off and if too cold it switches on. Cheapest thermostat controller u can find. $34...
|
|